PROTEIN-BASED OIL - ENCAPSULATES
    4.
    发明申请
    PROTEIN-BASED OIL - ENCAPSULATES 审中-公开
    基于蛋白质的油 - 包封

    公开(公告)号:WO2009070010A1

    公开(公告)日:2009-06-04

    申请号:PCT/NL2008/000264

    申请日:2008-12-01

    摘要: The invention provides an encapsulate comprising a protein-based encapsulation matrix that envelops one or more oil droplets containing at least 3 wt.% of polyunsaturated fatty acids (PUFAs) by weight of oil; said encapsulate having a mass weighted average diameter in the range of 0.5-5000 μm, wherein the protein-based encapsulation matrix contains at least 10 wt.% of a protein that has been cross-linked by means disulfide cross-links, said protein-based matrix further being characterized in that: i. less than 75 wt.%, preferably less than 40 wt.% of the protein contained in the protein-based matrix can be dissolved when 75 mg of the encapsulate is dispersed in 50 ml distilled water having a temperature of 5°C at any pH within the range of 3.0-7.0; ii. the weight percentage of the protein that can be dissolved is at least a factor 1.3 higher when in the procedure under i) the distilled water is replaced by an aqueous solution of 2 wt.% dithiothreitol (DTT). The encapsulate according to the present exhibits very high oxidative stability and can be produced via a robust and relatively simple manufacturing process. The invention also provides a process for the manufacture of the aforementioned encapsulate.

    摘要翻译: 本发明提供了包含基于蛋白质的包封基质的包封物,该基质包裹含有至少3重量%油重量的多不饱和脂肪酸(PUFA)的一个或多个油滴; 所述封装物具有在0.5-5000μm范围内的质量加权平均直径,其中基于蛋白质的封装基质含有至少10重量%的已经通过二硫键交联而交联的蛋白质,所述蛋白质 - 其特征在于:i。 当将75mg包封物分散在温度为5℃的50ml蒸馏水中时,可以溶解基于蛋白质的基质中所含的蛋白质的小于75重量%,优选小于40重量% 在3.0-7.0的范围内; II。 当在i)蒸馏水被2重量%二硫苏糖醇(DTT)的水溶液代替时,可以溶解的蛋白质的重量百分比至少为1.3倍。 根据本发明的包封物显示出非常高的氧化稳定性,并且可以通过鲁棒且相对简单的制造方法制备。 本发明还提供了制造上述封装体的方法。

    PROTEIN-BASED PROBIOTIC ENCAPSULATES
    5.
    发明申请
    PROTEIN-BASED PROBIOTIC ENCAPSULATES 审中-公开
    基于蛋白质的真菌包囊

    公开(公告)号:WO2009070012A1

    公开(公告)日:2009-06-04

    申请号:PCT/NL2008/000266

    申请日:2008-12-01

    摘要: The invention provides an encapsulate comprising a protein-based encapsulation matrix that envelops one or more probiotic bacteria, preferably Gram-positive probiotic bacteria; said encapsulate having a mass weighted average diameter in the range of 1-5000 μm, wherein the protein-based encapsulation matrix contains at least 10 wt.% of a protein that has been cross- linked by means disulfide cross-links, said protein-based matrix further being characterized in that: (i) less than 75 wt.%, preferably less than 40 wt.% of the protein contained in the protein-based matrix can be dissolved when 75 mg of the encapsulate is dispersed in 50 ml distilled water having a temperature of 5°C at any pH within the range of 3.0-7.0; and (ii) the weight percentage of the protein that can be dissolved is at least a factor 1.3 higher when in the procedure under i) the distilled water is replaced by an aqueous solution of 2 wt.% dithiothreitol (DTT). The encapsulate according to the present exhibits high survival rate when digested, and can be produced via a robust and relatively simple manufacturing process. The invention also provides a process for the manufacture of the aforementioned encapsulate.

    摘要翻译: 本发明提供了一种包封物,其包含包围一种或多种益生菌,优选革兰阳性益生菌的基于蛋白质的包封基质; 所述封装物具有在1-5000μm范围内的质量加权平均直径,其中基于蛋白质的封装基质含有至少10重量%的通过二硫键交联而交联的蛋白质,所述蛋白质 - 其特征在于:当将75mg包封物分散在50ml蒸馏物中时,可以将(i)小于75重量%,优选小于40重量%的蛋白质基质中所含蛋白质溶解。 在3.0-7.0范围内任何pH值的温度为5℃的水; 和(ii)当在i)蒸馏水被2重量%二硫苏糖醇(DTT)的水溶液代替时,可以溶解的蛋白质的重量百分比至少为1.3倍。 根据本发明的封装物在消化时表现出高的存活率,并且可以通过鲁棒且相对简单的制造工艺生产。 本发明还提供了制造上述封装体的方法。

    OXIDATIVELY CROSS-LINKED PROTEIN-BASED ENCAPSULATES
    6.
    发明申请
    OXIDATIVELY CROSS-LINKED PROTEIN-BASED ENCAPSULATES 审中-公开
    基于交联蛋白的氧化物

    公开(公告)号:WO2008143507A2

    公开(公告)日:2008-11-27

    申请号:PCT/NL2008/050299

    申请日:2008-05-21

    IPC分类号: B01J13/04 A23P1/04 A23L1/22

    摘要: The present invention provides a method of producing a protein-based encapsulate, said method comprising: providing an aqueous solution of a protein that is capable of forming disulfide cross-links; submitting said aqueous solution to a protein activation treatment to produce an aqueous suspension of activated protein aggregates, said suspension having a reactivity of at least 5.0 μmol thiol groups per gram of activated protein aggregates as determined in the Ellman's assay; dispensing said aqueous suspension in a gas or a water-immiscible liquid to produce suspension droplets having a diameter of 0.1-500 μm; and forming disulfide cross-links between the activated protein aggregates by contacting the activated protein aggregates with an oxidizing agent, optionally after said activated protein aggregates have been partially cross-linked by forming disulfide cross-links by means of heat treatment or by pressurization to a pressure in excess of 50 MPa. The aforementioned method offers the advantage that the characteristics of the protein-based encapsulation matrix can be controlled effectively. Furthermore, said method enables the preparation of protein-based encapsulates that very effectively protect the encapsulated components, e.g. against oxidation or moisture.

    摘要翻译: 本发明提供一种生产蛋白质包封物的方法,所述方法包括:提供能够形成二硫键交联的蛋白质的水溶液; 将所述水溶液提交至蛋白质活化处理以产生活化蛋白质聚集体的水性悬浮液,所述悬浮液具有至少5.0μmol硫醇基团的活性,每克活化蛋白质聚集体如Ellman测定中所测定; 将所述水性悬浮液分散在气体或与水不混溶的液体中以产生直径为0.1-500μm的悬浮液滴; 并且通过使活化的蛋白质聚集体与氧化剂接触,任选地在所述活化的蛋白质聚集体已经通过热处理或通过加压形成二硫化物交联部分交联之后,在活化的蛋白质聚集体之间形成二硫键交联 压力超过50MPa。 上述方法提供了可以有效地控制基于蛋白质的封装基质的特征的优点。 此外,所述方法能够制备基于蛋白质的包封物,其非常有效地保护包封的组分,例如, 防止氧化或潮湿。

    AN EDIBLE PRODUCT COMPOSED OF A PLURALITY OF DISCRETE FAT PIECES
    7.
    发明申请
    AN EDIBLE PRODUCT COMPOSED OF A PLURALITY OF DISCRETE FAT PIECES 审中-公开
    由多种不同脂肪组成的食品

    公开(公告)号:WO2011084059A1

    公开(公告)日:2011-07-14

    申请号:PCT/NL2011/050011

    申请日:2011-01-06

    IPC分类号: A23D7/005 A23D9/007 A23D9/05

    摘要: The present invention relates to an edible product that is composed of a plurality of discrete fat pieces that are in direct contact with each other, such as fat flakes, fat powders or fat granulates. More particularly, the present invention provides an edible product that is composed of a plurality of discrete fat pieces that are in direct contact with each other, each fat piece comprising: 15-80 wt.% of a solid continuous fat phase having a solid fat content at 25 ºC (N 2 ) of at least 5 wt.%; 20-85 wt.% of an oil encapsulate that is homogeneously dispersed throughout the continuous fat phase, said oil encapsulate containing 40-95 wt.% of liquid oil and 5-60 wt.% of an encapsulation matrix that envelops the liquid oil; and 0-50 wt.% of a dispersed aqueous phase; wherein the edible product contains at least 15% of liquid oil by weight of the continuous fat phase and wherein the continuous fat phase, the oil encapsulate and the optional dispersed aqueous phase together represent at least 90 wt.% of the edible product. The fat pieces in the edible fat product according to the invention, despite containing a substantial amount of liquid oil, do not clump together and can suitably be used in a variety of product applications, such as bakery products, soups, sauces, creamers and reconstitutable drinks.

    摘要翻译: 本发明涉及由脂肪片,脂肪粉或脂肪颗粒直接接触的多个离散的脂肪片构成的食用食品。 更具体地说,本发明提供一种可食用产品,其由彼此直接接触的多个离散的脂肪片组成,每个脂肪片包括:15-80重量%的具有固体的固体连续脂肪相 25℃(N 2)的脂肪含量至少为5重量%; 20-80重量%的油包封物均匀地分散在整个连续的脂肪相中,所述油包封含有40-95重量%的液体油和5-60重量%的包封液体油的包封基质 ; 和0〜50重量%的分散水相; 其中所述可食用产品含有连续脂肪相重量的至少15%的液体油,并且其中所述连续脂肪相,油包封物和任选的分散的水相一起代表可食用产品的至少90重量%。 根据本发明的可食用脂肪产品中的脂肪块,尽管含有大量的液体油,不会聚集在一起,并且可以适当地用于各种产品应用中,例如面包店产品,汤,调味汁,奶油和可重构的 饮料。

    OXIDATIVELY CROSS-LINKED PROTEIN-BASED ENCAPSULATES
    8.
    发明申请
    OXIDATIVELY CROSS-LINKED PROTEIN-BASED ENCAPSULATES 审中-公开
    基于交联蛋白的氧化物

    公开(公告)号:WO2008143507A3

    公开(公告)日:2009-04-23

    申请号:PCT/NL2008050299

    申请日:2008-05-21

    摘要: The present invention provides a method of producing a protein-based encapsulate, said method comprising: providing an aqueous solution of a protein that is capable of forming disulfide cross-links; submitting said aqueous solution to a protein activation treatment to produce an aqueous suspension of activated protein aggregates, said suspension having a reactivity of at least 5.0 µmol thiol groups per gram of activated protein aggregates as determined in the Ellman's assay; dispensing said aqueous suspension in a gas or a water-immiscible liquid to produce suspension droplets having a diameter of 0.1-500 µm; and forming disulfide cross-links between the activated protein aggregates by contacting the activated protein aggregates with an oxidizing agent, optionally after said activated protein aggregates have been partially cross-linked by forming disulfide cross-links by means of heat treatment or by pressurization to a pressure in excess of 50 MPa. The aforementioned method offers the advantage that the characteristics of the protein-based encapsulation matrix can be controlled effectively. Furthermore, said method enables the preparation of protein-based encapsulates that very effectively protect the encapsulated components, e.g. against oxidation or moisture.

    摘要翻译: 本发明提供一种生产蛋白质包封物的方法,所述方法包括:提供能够形成二硫键交联的蛋白质的水溶液; 将所述水溶液提交至蛋白质活化处理以产生活化蛋白质聚集体的水性悬浮液,所述悬浮液具有至少5.0μmol硫醇基团的活性,每克活化蛋白质聚集体如Ellman测定中所测定; 将所述水性悬浮液分散在气体或与水不混溶的液体中以产生直径为0.1-500μm的悬浮液滴; 并且通过使活化的蛋白质聚集体与氧化剂接触,任选地在所述活化的蛋白质聚集体已经通过热处理或通过加压形成二硫化物交联部分交联之后,在活化的蛋白质聚集体之间形成二硫键交联 压力超过50MPa。 上述方法提供了可以有效地控制基于蛋白质的封装基质的特征的优点。 此外,所述方法能够制备基于蛋白质的包封物,其非常有效地保护包封的组分,例如, 防止氧化或潮湿。