公开(公告)号：WO2010033140A2

公开(公告)日：2010-03-25

申请号：PCT/US2009002756

申请日：2009-05-05

Applicant: INNOVATIVE MICRO TECHNOLOGY ,  BISHOP JAMIE H ,  ERLACH DAVID M ,  FOSTER IAN S ,  FOSTER JOHN S ,  HARLEY JOHN C ,  THOMPSON DOUGLAS L

Inventor： BISHOP JAMIE H ,  ERLACH DAVID M ,  FOSTER IAN S ,  FOSTER JOHN S ,  HARLEY JOHN C ,  THOMPSON DOUGLAS L

IPC: H01J49/24

CPC classification number: G01N15/00 ,  B01L3/502761 ,  B01L3/505 ,  G01N2015/0065 ,  G01N2015/0288 ,  G01N2015/1409 ,  Y10T29/49002 ,  Y10T29/49815 ,  Y10T29/49817

Abstract: A micromechanical particle sorting system uses a removable/disposable apparatus which may include a compressible device, a filter apparatus and a cell sorter chip assembly. The chip assembly may include a tubing strain relief manifold and a microfabricated cell sorting chip. The chip assembly may be detachable from the filter apparatus in order to mount the MEMS particle sorting chip adjacent to a force- generating apparatus which resides with the particle sorting system. A disturbance device installed in the particle sorting system may interact with a transducer on the removable/disposable apparatus to reduce clogging of the flow through the system. Using this removable/disposable apparatus, when the sample is changed, the entire apparatus can be thrown away with minimal expense and system down time.

Abstract translation: 微机械颗粒分选系统使用可移除/一次性装置，其可包括可压缩装置，过滤器装置和细胞分选器芯片组件。 芯片组件可以包括管应变消除歧管和微制造的细胞分选芯片。 芯片组件可以从过滤装置上拆卸下来，以便将MEMS颗粒分选芯片安装在与颗粒分选系统所在的力产生装置相邻处。 安装在颗粒分选系统中的干扰装置可以与可移除/一次性装置上的换能器相互作用，以减少通过系统的流动的堵塞。 使用这种可拆卸/一次性设备，当样品被更换时，整个设备可以以最小的花费和系统停机时间被扔掉。

公开(公告)号：WO2006127256A2

公开(公告)日：2006-11-30

申请号：PCT/US2006/017743

申请日：2006-05-05

Applicant: INNOVATIVE MICRO TECHNOLOGY ,  FOSTER, John, S. ,  HOVEY, Steven, H. ,  MARTIN, Richard, T. ,  RUBEL, Paul, J. ,  NGUYEN, Hung, D. ,  HARLEY, John, C.

Inventor： FOSTER, John, S. ,  HOVEY, Steven, H. ,  MARTIN, Richard, T. ,  RUBEL, Paul, J. ,  NGUYEN, Hung, D. ,  HARLEY, John, C.

IPC: B01D63/00

CPC classification number: B01D61/14 ,  B01D63/00 ,  B01D65/08 ,  B01D67/0062 ,  B01D69/02 ,  B01D2321/2058 ,  G01N1/4077

Abstract: A microfabricated cross flow filter may have multiple filtration stages. The filtration stages may include microfabricated filter barriers and gaps created in a substrate, thereby allowing very tight tolerances in the filter barrier and gap dimensions to be maintained. Using the microfabrication techniques, the filter barriers can be made having arbitrary shapes, and arranged at an angle or curved with respect to the flow direction, making the pressure drop across the filtration stage more uniform in the cross flow direction.

Abstract translation: 微制造的交叉流过滤器可以具有多个过滤阶段。 过滤阶段可以包括微制造的过滤器屏障和在衬底中产生的间隙，从而允许保持过滤器屏障和间隙尺寸的非常严格的公差。 使用微细加工技术，过滤器屏障可以制成具有任意形状，并且以相对于流动方向成一定角度或弯曲的方式布置，使得横跨过滤台的压降在横向方向上更均匀。

公开(公告)号：WO2013112120A1

公开(公告)日：2013-08-01

申请号：PCT/US2012/000321

申请日：2012-07-11

Applicant: OWL BIOMEDICAL, INC. ,  INNOVATIVE MICRO TECHNOLOGY ,  FOSTER, John, S. ,  GRUMMITT, Daryl, W. ,  HARLEY, John, C. ,  LINTON, James, P. ,  SPONG, Jaquelin, K.

Inventor： FOSTER, John, S. ,  GRUMMITT, Daryl, W. ,  HARLEY, John, C. ,  LINTON, James, P. ,  SPONG, Jaquelin, K.

IPC: A61J1/06

CPC classification number: C12M47/04 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2400/043 ,  B01L2400/0439 ,  B01L2400/0478 ,  B01L2400/0481 ,  G01N15/1459 ,  G01N2015/149

Abstract: A disposable cartridge is described which is compatible with a MEMS particle sorting device. The disposable cartridge may include passageways which connect fluid reservoirs in the cartridge with corresponding microfluidic passageways on the MEMS chip. A flexible gasket may prevent leakages and allow the fluid to cross the gasket barrier through a plurality of holes in the gasket. Vents and septums may also be included to allow air to escape and fluids to be inserted by hypodermic needle. A MEMS-based particle sorting system using the disposable cartridge is also described.

Abstract translation: 描述了与MEMS颗粒分选装置兼容的一次性盒。 一次性盒可以包括将盒中的流体储存器与MEMS芯片上的相应微流体通道连接的通道。 柔性垫片可以防止泄漏，并允许流体通过垫圈中的多个孔穿过垫圈屏障。 还可以包括通气孔和隔膜，以允许空气逸出，并由皮下注射针插入液体。 还描述了使用一次性盒的基于MEMS的颗粒分选系统。

公开(公告)号：WO2007019103A2

公开(公告)日：2007-02-15

申请号：PCT/US2006/029661

申请日：2006-07-31

Applicant: INNOVATIVE MICRO TECHNOLOGY ,  FOSTER, John, S. ,  HARLEY, John, C. ,  HOVEY, Steven, H. ,  MARTIN, Richard, T. ,  NGUYEN, Hung ,  RUBEL, Paul, J.

Inventor： FOSTER, John, S. ,  HARLEY, John, C. ,  HOVEY, Steven, H. ,  MARTIN, Richard, T. ,  NGUYEN, Hung ,  RUBEL, Paul, J.

IPC: C12Q1/00 ,  C12M1/34 ,  H01L21/00

CPC classification number: C12M47/04 ,  A61M1/36 ,  A61M1/3633 ,  A61M1/3678 ,  A61M2202/0437 ,  B01L3/5027 ,  G01N21/6428 ,  G01N2015/0288 ,  G01N2015/149 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/2575

Abstract: A micromechanical particle sorting chip uses laser light directed through at least one of a reflective and refractive surface to come to a focus in an optically transparent layer. The laser light impinges on a particle of interest, causing it to fluoresce. Upon detecting the fluorescence, a micromemchanical actuator is activated, which directs the particle of interest into one of a plurality of possible exit paths.

Abstract translation: 微机械粒子分选芯片使用通过反射和折射表面中的至少一个引导的激光在光学透明层中进行聚焦。 激光照射在感兴趣的颗粒上，使其发出荧光。 在检测到荧光时，微机械致动器被激活，其将感兴趣的颗粒引导到多个可能的出口路径中的一个。

公开(公告)号：WO2013112121A1

公开(公告)日：2013-08-01

申请号：PCT/US2012/000322

申请日：2012-07-11

Applicant: OWL BIOMEDICAL, INC ,  INNOVATIVE MICRO TECHNOLOGY ,  FOSTER, John, S. ,  GRUMMIT, Daryl, W. ,  HARLEY, John, C. ,  SPONG, Jaquelin, K. ,  TURNER, Kimberly

Inventor： FOSTER, John, S. ,  GRUMMIT, Daryl, W. ,  HARLEY, John, C. ,  SPONG, Jaquelin, K. ,  TURNER, Kimberly

IPC: C12M1/34

CPC classification number: G01N33/5005 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2400/043 ,  B01L2400/0633 ,  C12M47/04

Abstract: A MEMS-based system and a method are described for separating a target particle from the remainder of a fluid stream. The system makes use of a unique, microfabricated movable structure formed on a substrate, which moves in a rotary fashion about one or more fixed points, which are all located on one side of the axis of motion. The movable structure is actuated by a separate force-generating apparatus, which is entirely separate from the movable structure formed on its substrate. This allows the movable structure to be entirely submerged in the sample fluid.

Abstract translation: 描述了基于MEMS的系统和方法，用于将目标颗粒与流体流的其余部分分离。 该系统利用形成在基板上的独特的微加工可移动结构，其以旋转方式围绕一个或多个固定点移动，所述固定点都位于运动轴的一侧。 可移动结构由独立的力产生装置致动，该装置与其基板上形成的可移动结构完全分开。 这允许可移动结构完全浸没在样品流体中。

公开(公告)号：WO2010033140A3

公开(公告)日：2010-03-25

申请号：PCT/US2009/002756

申请日：2009-05-05

Applicant: INNOVATIVE MICRO TECHNOLOGY ,  BISHOP, Jamie, H. ,  ERLACH, David, M. ,  FOSTER, Ian, S. ,  FOSTER, John, S. ,  HARLEY, John, C. ,  THOMPSON, Douglas, L.

Inventor： BISHOP, Jamie, H. ,  ERLACH, David, M. ,  FOSTER, Ian, S. ,  FOSTER, John, S. ,  HARLEY, John, C. ,  THOMPSON, Douglas, L.

IPC: B07C5/344

Abstract: A micromechanical particle sorting system uses a removable/disposable apparatus which may include a compressible device, a filter apparatus and a cell sorter chip assembly. The chip assembly may include a tubing strain relief manifold and a microfabricated cell sorting chip. The chip assembly may be detachable from the filter apparatus in order to mount the MEMS particle sorting chip adjacent to a force- generating apparatus which resides with the particle sorting system. A disturbance device installed in the particle sorting system may interact with a transducer on the removable/disposable apparatus to reduce clogging of the flow through the system. Using this removable/disposable apparatus, when the sample is changed, the entire apparatus can be thrown away with minimal expense and system down time.

公开(公告)号：WO2006127256A3

公开(公告)日：2009-04-16

申请号：PCT/US2006017743

申请日：2006-05-05

Applicant: INNOVATIVE MICRO TECHNOLOGY ,  FOSTER JOHN S ,  HOVEY STEVEN H ,  MARTIN RICHARD T ,  RUBEL PAUL J ,  NGUYEN HUNG D ,  HARLEY JOHN C

Inventor： FOSTER JOHN S ,  HOVEY STEVEN H ,  MARTIN RICHARD T ,  RUBEL PAUL J ,  NGUYEN HUNG D ,  HARLEY JOHN C

IPC: B01D63/00

CPC classification number: B01D61/14 ,  B01D63/00 ,  B01D65/08 ,  B01D67/0062 ,  B01D69/02 ,  B01D2321/2058 ,  G01N1/4077

Abstract: A microfabricated cross flow filter may have multiple filtration stages. The filtration stages may include microfabricated filter barriers and gaps created in a substrate, thereby allowing very tight tolerances in the filter barrier and gap dimensions to be maintained. Using the microfabrication techniques, the filter barriers can be made having arbitrary shapes, and arranged at an angle or curved with respect to the flow direction, making the pressure drop across the filtration stage more uniform in the cross flow direction.

Abstract translation: 微制造的交叉流过滤器可以具有多个过滤阶段。 过滤阶段可以包括微制造的过滤器屏障和在衬底中产生的间隙，从而允许保持过滤器屏障和间隙尺寸的非常严格的公差。 使用微细加工技术，过滤器屏障可以制成具有任意形状，并且以相对于流动方向成一定角度或弯曲的方式布置，使跨越过滤台的压降在横向方向上更均匀。

公开(公告)号：WO2007019103A3

公开(公告)日：2007-07-05

申请号：PCT/US2006029661

申请日：2006-07-31

Applicant: INNOVATIVE MICRO TECHNOLOGY ,  FOSTER JOHN S ,  HARLEY JOHN C ,  HOVEY STEVEN H ,  MARTIN RICHARD T ,  NGUYEN HUNG ,  RUBEL PAUL J

Inventor： FOSTER JOHN S ,  HARLEY JOHN C ,  HOVEY STEVEN H ,  MARTIN RICHARD T ,  NGUYEN HUNG ,  RUBEL PAUL J

IPC: G01N1/10 ,  A61M1/36 ,  B01L3/00 ,  C12M1/34 ,  C12Q1/00 ,  G01N15/02 ,  G01N21/05 ,  G01N21/64 ,  H01L21/00

CPC classification number: C12M47/04 ,  A61M1/36 ,  A61M1/3633 ,  A61M1/3678 ,  A61M2202/0437 ,  B01L3/5027 ,  G01N21/6428 ,  G01N2015/0288 ,  G01N2015/149 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/2575

Abstract: A micromechanical particle sorting chip (300) uses laser light (305) directed through at least one of a reflective (360) and refractive (310) surface to come to a focus (320) in an optically transparent layer. The laser light (305) impinges on a particle of interest, causing it to fluoresce. Upon detecting the fluorescence, a micromechanical actuator (400) is activated, which directs the particle of interest into one of a plurality of possible exit paths.

Abstract translation: 微机械粒子分选芯片（300）使用穿过反射（360）和折射（310）表面中的至少一个的激光（305）到达光学透明层中的聚焦（320）。 激光（305）撞击感兴趣的颗粒，使其发荧光。 在检测到荧光时，微机械致动器（400）被激活，其将感兴趣的颗粒引导到多个可能的出口路径中的一个。

公开(公告)号：WO2007053281A3

公开(公告)日：2007-06-28

申请号：PCT/US2006040015

申请日：2006-10-12

Applicant: INNOVATIVE MICRO TECHNOLOGY ,  FOSTER JOHN S ,  HARLEY JOHN C ,  MARTIN RICHARD T ,  NGUYEN HUNG ,  RUBEL PAUL J

Inventor： FOSTER JOHN S ,  HARLEY JOHN C ,  MARTIN RICHARD T ,  NGUYEN HUNG ,  RUBEL PAUL J

IPC: G01N1/10 ,  A61M1/36 ,  B01L3/00 ,  C12M1/34 ,  C12Q1/00 ,  G01N15/02 ,  G01N21/05 ,  G01N21/64 ,  H01L21/00

CPC classification number: B01L3/502707 ,  A61M1/3633 ,  A61M1/3678 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0864 ,  B01L2400/0484 ,  C12M47/04 ,  G01N21/6428 ,  G01N2015/0288 ,  G01N2015/149 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/2575

Abstract: A micromechanical particle sorting chip uses an actuator divided into two parts to direct a component of interest into one of a plurality of possible exit paths, based on detection of a fluorescent signal emanating from the component of interest. The two-part actuator may include a force-generating portion (350) and a microactuator portion (330). The microactuator portion (330) may be disposable, whereas the force-generating portion (350) may be reuseable. By bringing the force-generating portion (350) into proximity to the microactuator portion (330), the microactuator (300) is induced to move, thereby separating the component of interest from the rest of the fluid stream. The force-generating portion (350) and the microactuator portion (330) may be optimized and fabricated separately, thereby leading to faster, more reliable and less expensive particle sorting.

Abstract translation: 微机械粒子分选芯片基于从感兴趣的部件发出的荧光信号的检测，使用被分成两部分的致动器将感兴趣的部件引导到多个可能的出口路径中的一个。 两部分致动器可以包括力产生部分（350）和微致动器部分（330）。 微致动器部分（330）可以是一次性的，而力产生部分（350）可以是可再利用的。 通过使力产生部分（350）接近微致动器部分（330），微致动器（300）被诱导移动，从而将感兴趣的部件与其余的流体流分离。 力产生部分（350）和微致动器部分（330）可以被分开地优化和制造，从而导致更快，更可靠和更便宜的颗粒分选。

公开(公告)号：WO2007053281A2

公开(公告)日：2007-05-10

申请号：PCT/US2006/040015

申请日：2006-10-12

Applicant: INNOVATIVE MICRO TECHNOLOGY ,  FOSTER, John, S. ,  HARLEY, John, C. ,  MARTIN, Richard, T. ,  NGUYEN, Hung ,  RUBEL, Paul, J.

Inventor： FOSTER, John, S. ,  HARLEY, John, C. ,  MARTIN, Richard, T. ,  NGUYEN, Hung ,  RUBEL, Paul, J.

IPC: B01L3/00

CPC classification number: B01L3/502707 ,  A61M1/3633 ,  A61M1/3678 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0864 ,  B01L2400/0484 ,  C12M47/04 ,  G01N21/6428 ,  G01N2015/0288 ,  G01N2015/149 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/2575

Abstract: A micromechanical particle sorting chip uses an actuator divided into two parts to direct a component of interest into one of a plurality of possible exit paths, based on detection of a fluorescent signal emanating from the component of interest. The two-part actuator may include a force-generating portion and a microactuator portion. The microactuator portion may be disposable, whereas the force-generating portion may be reuseable. By bringing the force-generating portion into proximity to the microactuator portion, the microactuator is induced to move, thereby separating the component of interest from the rest of the fluid stream. The force-generating portion and the microactuator portion may be optimized and fabricated separately, thereby leading to faster, more reliable and less expensive particle sorting.

Abstract translation: 微机械粒子分选芯片基于从感兴趣的部件发出的荧光信号的检测，使用被分成两部分的致动器将感兴趣的部件引导到多个可能的出口路径中的一个。 两部分致动器可以包括力产生部分和微致动器部分。 微致动器部分可以是一次性的，而力产生部分可以是可再利用的。 通过使力产生部分靠近微致动器部分，微致动器被诱导移动，从而将感兴趣的部件与流体流的其余部分分离。 力产生部分和微致动器部分可以分开优化和制造，从而导致更快，更可靠和更便宜的颗粒分选。