公开(公告)号：WO2008002882A2

公开(公告)日：2008-01-03

申请号：PCT/US2007/072047

申请日：2007-06-25

Applicant: BLOOD CELL STORAGE, INC. ,  REED, Michael, W. ,  NANASSY, Olivier, Z. ,  HAYDOCK, Paul, V. ,  SHARMA, Nigel, R. ,  BARDELL, Ronald, L. ,  HARGRAVE, Perry

Inventor： REED, Michael, W. ,  NANASSY, Olivier, Z. ,  HAYDOCK, Paul, V. ,  SHARMA, Nigel, R. ,  BARDELL, Ronald, L. ,  HARGRAVE, Perry

IPC: C12Q1/68 ,  B01L3/00

CPC classification number: C12Q1/6851 ,  B01L3/5027 ,  B01L3/50273 ,  B01L7/525 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/0457 ,  B01L2400/0487 ,  C12Q1/6806 ,  C12Q1/686 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2565/137 ,  C12Q2563/173

Abstract: Device and methods for extracting and analyzing nucleic acids from biological samples.

Abstract translation: 从生物样品中提取和分析核酸的装置和方法。

公开(公告)号：WO2002098895A1

公开(公告)日：2002-12-12

申请号：PCT/US2002/018229

申请日：2002-06-07

Applicant: SAIGENE CORPORATION ,  HAYDOCK, Paul, V. ,  U'REN, Jack

Inventor： HAYDOCK, Paul, V. ,  U'REN, Jack

IPC: C07H21/02

CPC classification number: C12Q1/6865 ,  C12Q2525/173 ,  C12Q2521/107

Abstract: This invention provides for a novel amplification procedure for nucleic acid. The method uses a wild type or mutant RNA polymerase designed to transcribe both deoxyribonucleotides and ribonucleotides (see figures 1-7).

Abstract translation: 本发明提供了核酸的新型扩增方法。 该方法使用设计用于转录脱氧核糖核苷酸和核糖核苷酸的野生型或突变型RNA聚合酶（参见图1-7）。

公开(公告)号：WO2009117167A1

公开(公告)日：2009-09-24

申请号：PCT/US2009/030051

申请日：2009-01-02

Applicant: BLOOD CELL STORAGE, INC. ,  REED, Michael, W. ,  NANASSY, Oliver, Z. ,  HAYDOCK, Paul, V. ,  SHARMA, Nigel, Rudra ,  BARDELL, Ronald, L. ,  HARGRAVE, Perry

Inventor： REED, Michael, W. ,  NANASSY, Oliver, Z. ,  HAYDOCK, Paul, V. ,  SHARMA, Nigel, Rudra ,  BARDELL, Ronald, L. ,  HARGRAVE, Perry

IPC: C12M1/00 ,  B01D11/04 ,  C07H21/00

CPC classification number: C12Q1/6834 ,  B01L3/5027 ,  B01L3/502715 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0688 ,  B01L2400/084 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q2565/629 ,  C12Q2563/173 ,  C12Q2565/137

Abstract: Devices, processes, and kits for the extraction of nucleic acids from biological samples are disclosed. The devices comprise a first port, a second port, and a binding chamber intermediate and in fluid communication with the first port and the second port. The binding chamber comprises an unmodified flat glass surface effective for binding a heterogeneous population of nucleic acids. The first port, second port, and binding chamber define a continuous fluid pathway that is essentially free of nucleic acid-specific binding sites.

Abstract translation: 公开了用于从生物样品中提取核酸的装置，方法和试剂盒。 这些装置包括中间并与第一端口和第二端口流体连通的第一端口，第二端口和装订室。 结合室包含有效用于结合异质核酸群体的未改性平板玻璃表面。 第一端口，第二端口和结合室限定基本上不含核酸特异性结合位点的连续流体通路。

公开(公告)号：WO2012075508A3

公开(公告)日：2012-06-07

申请号：PCT/US2011/063337

申请日：2011-12-05

Applicant: BLOOD CELL STORAGE, INC. ,  HAYDOCK, Paul, V.

Inventor： HAYDOCK, Paul, V.

IPC: C12Q1/02 ,  C12Q1/24 ,  C12Q1/04 ,  C12N11/14 ,  C12Q1/68 ,  C12N1/20 ,  C12N1/16 ,  G01N33/569

Abstract: A process for isolating microorganisms is disclosed. The process utilizes a device comprising an inner surface, an outer surface, a first port, and a second port, wherein the inner surface comprises an unmodified, smooth glass substrate and defines a binding chamber providing fluid communication between the first port and second port. Microorganisms in an aqueous solution are contacted with the unmodified, smooth glass substrate, wherein the solution is essentially free of cell precipitants, and the microorganisms are allowed to bind to the glass substrate.

公开(公告)号：WO2010054004A2

公开(公告)日：2010-05-14

申请号：PCT/US2009063296

申请日：2009-11-04

Applicant: BLOOD CELL STORAGE INC ,  REED MICHAEL W ,  NANASSY OLIVER Z ,  HAYDOCK PAUL V ,  GESTWICK DANIEL P

Inventor： REED MICHAEL W ,  NANASSY OLIVER Z ,  HAYDOCK PAUL V ,  GESTWICK DANIEL P

IPC: C12N15/10 ,  B01L99/00 ,  C07H21/00 ,  C12M1/24 ,  C12Q1/68

CPC classification number: B01L3/5027 ,  B01L3/502715 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0688 ,  B01L2400/084 ,  C12Q1/6806 ,  C12Q1/6851 ,  Y02P20/582 ,  Y10T137/85986 ,  C12Q2565/629 ,  C12Q2565/137 ,  C12Q2563/173

Abstract: Processes for extracting nucleic acid from a biological sample and related assemblies and kits are disclosed. The processes comprise the steps of (a) providing a device comprising an inner surface, an outer surface, a first port, and a second port, wherein the inner surface is composed of unmodified, smooth glass and defines a tubular lumen providing fluid communication between the first port and second port, wherein the lumen is circular, oval, or elliptical in cross-section, and wherein the lumen is essentially free of nucleic acid-specific binding sites; (b) introducing a nucleic acid-containing sample into the lumen of the device via the first port; (c) allowing nucleic acid in the sample to bind to the unmodified smooth glass surface; and (d) washing the bound nucleic acid.

Abstract translation: 公开了用于从生物样品和相关组件和试剂盒中提取核酸的方法。 所述方法包括以下步骤：（a）提供包括内表面，外表面，第一端口和第二端口的装置，其中所述内表面由未改性的光滑玻璃组成，并限定提供流体连通的管状内腔 所述第一端口和所述第二端口，其中所述内腔的横截面为圆形，椭圆形或椭圆形，并且其中所述内腔基本上没有核酸特异性结合位点; （b）通过第一端口将含有核酸的样品引入装置的内腔; （c）使样品中的核酸与未修饰的平滑玻璃表面结合; 和（d）洗涤结合的核酸。

公开(公告)号：WO2008002882A3

公开(公告)日：2008-04-24

申请号：PCT/US2007072047

申请日：2007-06-25

Applicant: BLOOD CELL STORAGE INC ,  REED MICHAEL W ,  NANASSY OLIVIER Z ,  HAYDOCK PAUL V ,  SHARMA NIGEL R ,  BARDELL RONALD L ,  HARGRAVE PERRY

Inventor： REED MICHAEL W ,  NANASSY OLIVIER Z ,  HAYDOCK PAUL V ,  SHARMA NIGEL R ,  BARDELL RONALD L ,  HARGRAVE PERRY

IPC: C12Q1/68 ,  B01L3/00

CPC classification number: C12Q1/6851 ,  B01L3/5027 ,  B01L3/50273 ,  B01L7/525 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/0457 ,  B01L2400/0487 ,  C12Q1/6806 ,  C12Q1/686 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2565/137 ,  C12Q2563/173

Abstract: Device and methods for extracting and analyzing nucleic acids from biological samples.

Abstract translation: 用于从生物样品中提取和分析核酸的装置和方法。

公开(公告)号：WO2012075508A2

公开(公告)日：2012-06-07

申请号：PCT/US2011063337

申请日：2011-12-05

Applicant: BLOOD CELL STORAGE INC ,  HAYDOCK PAUL V

Inventor： HAYDOCK PAUL V

IPC: C12Q1/02 ,  C12N1/16 ,  C12N1/20 ,  C12N11/14 ,  C12Q1/04 ,  C12Q1/24 ,  C12Q1/68 ,  G01N33/569

CPC classification number: C12Q1/24 ,  C12Q1/04 ,  C12Q1/6806 ,  G01N33/569 ,  G01N33/56911

Abstract: A process for isolating microorganisms is disclosed. The process utilizes a device comprising an inner surface, an outer surface, a first port, and a second port, wherein the inner surface comprises an unmodified, smooth glass substrate and defines a binding chamber providing fluid communication between the first port and second port. Microorganisms in an aqueous solution are contacted with the unmodified, smooth glass substrate, wherein the solution is essentially free of cell precipitants, and the microorganisms are allowed to bind to the glass substrate.

Abstract translation: 公开了一种分离微生物的方法。 该方法利用包括内表面，外表面，第一端口和第二端口的装置，其中内表面包括未改性的光滑玻璃基材并且限定提供第一端口和第二端口之间的流体连通的结合室。 使水溶液中的微生物与未改性的光滑玻璃基质接触，其中该溶液基本不含细胞沉淀剂，并使微生物与玻璃基质结合。

公开(公告)号：WO2010054004A3

公开(公告)日：2010-05-14

申请号：PCT/US2009/063296

申请日：2009-11-04

Applicant: BLOOD CELL STORAGE, INC. ,  REED, Michael, W. ,  NANASSY, Oliver, Z. ,  HAYDOCK, Paul, V. ,  GESTWICK, Daniel, P.

Inventor： REED, Michael, W. ,  NANASSY, Oliver, Z. ,  HAYDOCK, Paul, V. ,  GESTWICK, Daniel, P.

IPC: C12N15/10 ,  C12Q1/68 ,  C07H21/00 ,  C12M1/24

Abstract: Processes for extracting nucleic acid from a biological sample and related assemblies and kits are disclosed. The processes comprise the steps of (a) providing a device comprising an inner surface, an outer surface, a first port, and a second port, wherein the inner surface is composed of unmodified, smooth glass and defines a tubular lumen providing fluid communication between the first port and second port, wherein the lumen is circular, oval, or elliptical in cross-section, and wherein the lumen is essentially free of nucleic acid-specific binding sites; (b) introducing a nucleic acid-containing sample into the lumen of the device via the first port; (c) allowing nucleic acid in the sample to bind to the unmodified smooth glass surface; and (d) washing the bound nucleic acid.

公开(公告)号：WO1997044489A1

公开(公告)日：1997-11-27

申请号：PCT/US1997008768

申请日：1997-05-22

Applicant: MONTEREY BAY AQUARIUM RESEARCH INSTITUTE ,  SCHOLIN, Christopher, A. ,  CANGELOSI, Gerard, A. ,  HAYDOCK, Paul, V.

Inventor： MONTEREY BAY AQUARIUM RESEARCH INSTITUTE

IPC: C12Q01/68

CPC classification number: C12Q1/6888 ,  C12Q1/6895

Abstract: The present invention provides compositions, methods, and kits for detecting species of Pseudo-nitzschia from a marine sample. Oligonucleotide probes for rRNA hypervariable regions of the Pseudo-nitzschia species: P. australis, P. pungens, P. multiseries, P. pseudodelicatissima, P. heimii, P. fraudulenta, P. delicatissima, and P. americana are provided as well as an oligonucleotide probe for a conserved region of ribosomal RNA from Pseudo-nitzschia.

Abstract translation: 本发明提供了用于从海洋样品中检测伪硝化物种的组合物，方法和试剂盒。 拟硝化物种的rRNA高变区的寡核苷酸探针：P. australis，P. pungens，P. multiseries，P. pseudodelicatissima，P. heimii，P. fraudulenta，P. delicatissima和P. americana都提供了 用于来自伪硝唑的核糖体RNA的保守区的寡核苷酸探针。