中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

14 records (took 0.01 seconds)

    VERFAHREN ZUR SEPARATION POLARISIERBARER BIOPARTIKEL
    1.
    发明申请
    VERFAHREN ZUR SEPARATION POLARISIERBARER BIOPARTIKEL 审中-公开

    公开(公告)号：WO2012152844A3

    公开(公告)日：2012-11-15

    申请号：PCT/EP2012/058575

    申请日：2012-05-09

    Applicant: TECHNISCHE UNIVERSITÄT DORTMUND ,  SCHMID, Andreas ,  BLANK, Lars M. ,  HOWITZ, Steffen ,  FRITZSCH, Frederik

    Inventor： SCHMID, Andreas ,  BLANK, Lars M. ,  HOWITZ, Steffen ,  FRITZSCH, Frederik

    IPC: B03C5/00 ,  B03C5/02

    Abstract: Die Erfindung betrifft ein Verfahren zur Separation eines polarisierbaren Biopartikels (130) umfassend die Schritte: a) dielektrophoretische Vorseparation eines polarisierbaren Biopartikels (130) aus einer Suspension von Biopartikeln; b) fluidische Separation des ausgewählten Biopartikels (130) durch Fixieren des Biopartikels (130) in einem dielektrophoretischen Feldkäfig (230) und Umströmen des Biopartikels (130) mit Fluid; c) Überführung des separierten Biopartikels (130) aus dem dielektrophoretischen Feldkäfig (230) in einen Kultivierungsraum (22); d) dielektrophoretisches Fixieren des separierten Biopartikels (130) in dem Kultivierungsraum (22) und Untersuchung, Beobachtung, Manipulation und/oder Kultivierung des separierten Biopartikels (130). Die Erfindung betrifft ferner ein mikrofluidisches System (1) und dessen Verwendung.

    ELECTRICALLY CONTROLLABLE MICRO-PIPETTE
    2.
    发明申请
    ELECTRICALLY CONTROLLABLE MICRO-PIPETTE 审中-公开
    电气可控PIPETTE MICRO

    公开(公告)号：WO1996024040A2

    公开(公告)日：1996-08-08

    申请号：PCT/DE1996000139

    申请日：1996-01-31

    Applicant: HOWITZ, Steffen ,  BÜRGER, Mario ,  WEGENER, Thomas

    IPC: G01N01/00

    CPC classification number: B01L3/0268 ,  B01L3/5027 ,  B01L9/52 ,  B01L2200/143 ,  B01L2400/0481 ,  B01L2400/084 ,  G01N35/1016 ,  G01N35/1095 ,  G01N2035/00237 ,  G01N2035/1039

    Abstract: The invention relates to an electrically controllable micro-pipette for handling extremely small volumes of fluid in the range from a few hundred pl to a few mu l. The micro-pipette makes it possible to take up fluids, fluid mixtures or the micro-particles, convey them off into the interior of complex systems and deposit them accurately at a sample processing point or dispose of them. The electrically controllable micro-pipette consists of a micro-diaphragm pump filled with an inert vehicle fluid used as a micro-injection pump (2) which comprises a micro-engineered chamber (7) with an elastic chamber wall (14) having an electrically controllable actuator (12), with the micro-discharge capillary tube (4) in the form of a pipette tip. The electrically controllable micro-pipette of the invention is made using micro-system engineering methods; it may be integrated into the micro-pipette system as a hybrid structure of several individual chips or compactly into a single silicon chip. Both constructions are essentially suitable for mass production and are distinguished by extremely high precision and reproducibility. The use of the micro-pipette of the invention results, depending on the application, in a reduction in the dead volume or media consumption. The absence of mechanically moved components makes it highly reliable and gives it a long maintenance-free useful life.

    Abstract translation: 本发明涉及一种电控制的微移液管用于在几百PL的范围小的流体体积的处理，以几个微米升。 微移液管允许流体，流体混合物或微粒的吸收可以包含在其中，其在复杂的系统的内部移除和有针对性的吸收的物质递送至样品或样品处理废物的部位。 可电控的微移液管包括一个填充有惰性载体液体如（2）中使用的微隔膜泵由微制造腔室（7）具有一个电可控的致动器（12）的弹性室壁（14）一个微喷射构造，其 Mikroauslaufkapillare（4）被设计成一个移液管尖端。 本发明的可电控的微移液管使用的微系统技术的技术来制备，它们可以被集成为包括多个单独的芯片到系统微移液管的混合结构，或者也可以紧凑地集成到只有一个硅芯片上。 基本上，无论是生产其他方案是适合于大规模生产，并通过非常高的精确度和再现的特征。 使用本发明的微量移液器引线，取决于应用到一个减少死体积的或媒体消费。 由于缺乏机械运动部件的它具有非常高的可靠性和免维护寿命。

    METHOD AND DEVICE FOR TRANSFERRING NANOSTRUCTURES BY MEANS OF CONTACT STAMPING
    4.
    发明申请
    METHOD AND DEVICE FOR TRANSFERRING NANOSTRUCTURES BY MEANS OF CONTACT STAMPING 审中-公开
    方法和装置用于转移微米或纳米结构通过以下方式联系TEMPLE

    公开(公告)号：WO2008154907A3

    公开(公告)日：2009-03-19

    申请号：PCT/DE2008001002

    申请日：2008-06-23

    Applicant: GESIM GES FUER SILIZIUM MIKROS ,  HOWITZ STEFFEN

    Inventor： HOWITZ STEFFEN

    IPC: G03F7/00 ,  B41F17/00

    CPC classification number: B41F17/001 ,  B82Y10/00 ,  B82Y40/00 ,  G03F7/0002

    Abstract: The invention relates to a method and a device for transferring micro- or nanostructures onto a substrate by contact stamping. The stamp is composed of a stamp receptacle and a lower stamp part, which are removably joined together. This permits both a rapid change of different lower stamp parts with different micro- or nanostructures and permits mechanical stress of the structure membranes to be avoided. The material to be printed is transferred from the source to the substrate by the wetting of the structure membrane with the substances to be transferred and by subsequently bringing the substrate into contact with an initial contact region located centrally on the structure membrane which grows concentrically towards the exterior. This achieves a reproducible transfer of substances. The convex deformation of the structure membrane is achieved by an excess pressure in the chamber formed by the stamp receptacle and the structure membrane, a gas or a liquid being supplied to the chamber via the media connection. If the structure membrane is permeable, the chamber has a reservoir function for the material to be transferred, said material diffusing through the structure membrane. Micro- or nano-arrays with different fine structures can be created on the substrate surface by a positioning mechanism comprising a marking system.

    Abstract translation: 本发明涉及一种方法和用于通过接触柱塞转印微或纳米结构的衬底的装置。 印模从凸模夹持器和可拆卸地连接在一起的模具下部形成。 其特征在于两个具有不同的微米或纳米结构不同的RAM部件之间的快速变化是可能的，以及避免膜的结构的机械负荷得以实现。 打印材料从源到基板的转移是通过加湿用物质膜结构被转移并从位于中央的对膜结构的初始接触面积到外部的基片的后续接触同心增加进行。 其特征在于所述物质的可重现的传输来实现的。 结构膜的凸变形是由凸模夹持器和结构隔膜室形成的基团，其中气体或液体供给到腔室中的介质连接器中的过压来实现。 在设计的透过膜的结构，要传送具有用于材料的贮存功能的腔室，其通过膜结构扩散。 通过用标记系统微米或纳米阵列的定位机构与所述基片表面上不同的微观结构生成。

    CRYO-PRESERVATION AND LOW-TEMPERATURE PROCESSING OF BIOLOGICAL OBJECTS
    5.
    发明申请
    CRYO-PRESERVATION AND LOW-TEMPERATURE PROCESSING OF BIOLOGICAL OBJECTS 审中-公开
    冷冻保存和生物对象的低温处理

    公开(公告)号：WO1996013159A1

    公开(公告)日：1996-05-09

    申请号：PCT/DE1995001490

    申请日：1995-10-26

    Applicant: FUHR, Günter ,  HORNUNG, Jan ,  HAGEDORN, Rolf ,  MÜLLER, Torsten ,  HOWITZ, Steffen

    IPC: A01N01/02

    CPC classification number: A01N1/02 ,  A01N1/0257 ,  A01N1/0278 ,  A01N1/0294

    Abstract: For the purposes of the located cryo-preservation of individual living biological objects (e.g. cells) or a predeterminable assembled number thereof, said objects are emptied from a storage container on a supercooled substrate (13) in an enveloping solution in microdrop form (12), e.g. via a microdropper (12). The substrate is brought to temperature via a coolant (15), with the surface to be covered in a gas atmosphere or a vacuum (14). The surface of the substrate is kept at a temperature T1 causing the impinging microdrops to freeze, while the substrate surface is microstructured for support and may contain measuring components. By suitably moving either the substrate or the microdropper it is possible to apply the microdrops individually and in samples in a freely selectable array or one predetermined by the substrate structure. The substrates with the applied microdrops and the cells frozen therein are stored at temperatures T2 of down to -273 DEG C and or subjected to a material-removing process with a treatment agent (17) or to a deposition treatment. It is possible in the deep-frozen state to perform manipulations like removal by mechanical action or the addition of solutions and substances to the surface of the substrate. On thawing, the substrate surface is taken to a temperature T1 above the freezing point of the enveloping solution and the microdrop array is thawed in a predeterminable manner.

    Abstract translation: 用于单个或在活的生物对象（例如，细胞）的一个预先确定的数量编译这些从一个储存容器，例如微滴射装置（11）的位置上定义的冷冻保存在微液滴形式的鞘液（12）冷冻基板（13）上卷起。 基板进行温度控制通过冷却装置（15），其中所述表面被定位在气体气氛中或在真空中（14）。 基板表面被保持在一个温度T1，这导致撞击微滴的凝固，其特征在于，所述基板表面是支持性微结构，并且可以包含传感元件。 由基板或拍摄组件的微滴的受控运动，微滴可在任意的或由衬底构图阵列单独地和图案施加预定的。 与沉积的微滴和所述冷冻的细胞的底物在温度T2存储在其中至多-273℃和/或与装置（17）或加工-auflagernd去除材料的机加工。 在冷冻状态下操作，如提取，通过机械去除或加入底物的表面上的解决方案和物质的情况下进行。 解冻时在衬底表面被带至温度T1包封溶液的凝固点以上，并在可预定的方式解冻微滴阵列。

    METHOD FOR THE SEPARATION OF POLARISABLE BIOPARTICLES
    6.
    发明申请
    METHOD FOR THE SEPARATION OF POLARISABLE BIOPARTICLES 审中-公开
    法分离极化生物颗粒

    公开(公告)号：WO2012152844A2

    公开(公告)日：2012-11-15

    申请号：PCT/EP2012058575

    申请日：2012-05-09

    Applicant: UNIV DORTMUND TECH ,  SCHMID ANDREAS ,  BLANK LARS M ,  HOWITZ STEFFEN ,  FRITZSCH FREDERIK

    Inventor： SCHMID ANDREAS ,  BLANK LARS M ,  HOWITZ STEFFEN ,  FRITZSCH FREDERIK

    IPC: B03C5/00

    CPC classification number: B03C5/005 ,  B01L3/50273 ,  B01L2400/0424 ,  B03C5/026 ,  B03C2201/26 ,  B81B7/02

    Abstract: The invention relates to a method for the separation of a polarisable bioparticle comprising the steps: a) dielectrophoretic preseparation of a polarisable bioparticle from a suspension of bioparticles; b) fluidic separation of the selected bioparticle by fixing the bioparticle in a dielectrophoretic field cage and circulating fluid around the bioparticle; c) transferring the separated bioparticle from the dielectrophoretic field cage to a culture chamber; d) dielectrophoretic fixing of the separated bioparticle in the culture chamber and study, observation, manipulation and/or culturing of the separated bioparticle. The invention further relates to a microfluidic system and use thereof.

    Abstract translation: 本发明涉及一种用于包括以下步骤的可极化Biopartikels的分离的方法：a）从悬浮液中的生物颗粒的介电泳极化Biopartikels的初步分离; B）通过在介电泳场笼固定Biopartikels和周围流体的流动Biopartikels所选Biopartikels的流体分离; C）从在培养空间的介电电泳场笼传送所述分离Biopartikels; D）固定介于培养室内和检查，观察，操纵和/或分离的Biopartikels的培养分离Biopartikels。 本发明还涉及一种微流体系统及其用途。

    ELECTRICALLY CONTROLLABLE MICRO-PIPETTE
    8.
    发明申请
    ELECTRICALLY CONTROLLABLE MICRO-PIPETTE 审中-公开
    电控可控微管

    公开(公告)号：WO9624040A3

    公开(公告)日：1996-09-26

    申请号：PCT/DE9600139

    申请日：1996-01-31

    Applicant: HOWITZ STEFFEN ,  BUERGER MARIO ,  WEGENER THOMAS

    Inventor： HOWITZ STEFFEN ,  BUERGER MARIO ,  WEGENER THOMAS

    IPC: B01L3/00 ,  B01L3/02 ,  B01L99/00 ,  G01N1/00 ,  G01N35/00 ,  G01N35/10

    CPC classification number: B01L3/0268 ,  B01L3/5027 ,  B01L9/52 ,  B01L2200/143 ,  B01L2400/0481 ,  B01L2400/084 ,  G01N35/1016 ,  G01N35/1095 ,  G01N2035/00237 ,  G01N2035/1039

    Abstract: The invention relates to an electrically controllable micro-pipette for handling extremely small volumes of fluid in the range from a few hundred pl to a few νl. The micro-pipette makes it possible to take up fluids, fluid mixtures or the micro-particles, convey them off into the interior of complex systems and deposit them accurately at a sample processing point or dispose of them. The electrically controllable micro-pipette consists of a micro-diaphragm pump filled with an inert vehicle fluid used as a micro-injection pump (2) which comprises a micro-engineered chamber (7) with an elastic chamber wall (14) having an electrically controllable actuator (12), with the micro-discharge capillary tube (4) in the form of a pipette tip. The electrically controllable micro-pipette of the invention is made using micro-system engineering methods; it may be integrated into the micro-pipette system as a hybrid structure of several individual chips or compactly into a single silicon chip. Both constructions are essentially suitable for mass production and are distinguished by extremely high precision and reproducibility. The use of the micro-pipette of the invention results, depending on the application, in a reduction in the dead volume or media consumption. The absence of mechanically moved components makes it highly reliable and gives it a long maintenance-free useful life.

    FLUID MICRO-DIODE
    9.
    发明申请
    FLUID MICRO-DIODE 审中-公开
    微流二极管

    公开(公告)号：WO1995022696A1

    公开(公告)日：1995-08-24

    申请号：PCT/DE1995000200

    申请日：1995-02-17

    Applicant: HOWITZ, Steffen ,  PHAM, Minh, Tan

    IPC: F15C04/00

    CPC classification number: B01F13/0059 ,  B01F5/0475 ,  B01L3/5027 ,  F15C4/00 ,  Y10T137/206 ,  Y10T137/2224 ,  Y10T137/87652

    Abstract: A fluid micro-diode for directionally coupling a dosed fluid to another stationary or flowing target fluid contained in a closed system, in particular in the sub-microliter range, is characterised by a planar arrangement of micro-capillaries open at both ends or by a system of micro-capillaries open at both ends and closely arranged next to each other, which are in direct contact with the target fluid at their outlet side and are separated at their inlet side from the dosed fluid to be intermittently supplied by an air or gas cushion, forming a meniscus (6) whose curvature depends on the surface tension. The fluid micro-diode forms a component (1) made of a sandwich arrangement of a flow channel (9), of the diode itself designed as a grid of capillaries and of a spacer chip (2) that secures the gaseous medium in the area of the coupling surface. These three stacked elements are produced as modules by micro-structuring techniques and may be integrated into micro-systems by building and joining techniques for micro-systems. The fluid micro-diode is characterised by a simple design and coupling flexibility to various micro-flow systems in which exists a hydrostatic pressure in the range of the prevailing ambient pressure.

    Abstract translation: 本发明涉及一种用于在亚微升范围执导耦合Dosierfluides到另一个站或在一个封闭的系统目标中流动的流体，befindliches，尤其是微流体二极管。 它的特点是在两侧微毛细管或两侧紧密并置的开放微毛细管，其与所述目标流体的输出侧直接接触，并通过从所述不连续地提供的剂量的流体的空气或气体缓冲的输入侧的一个系统的开放的平面排列以形成根据所述表面张力 弯曲的弯月面（6）是分开的。 组分（1）是从流路（9），在通过毛细管和一个Spacerchip形成的晶格结构的形式的实际二极管的堆叠组件中的微流体二极管（2），其固定所述气态介质在所述耦入面的面积。 这三个堆叠的元件被制造为具有微结构技术的技术的模块可以被集成到使用微封装和互连技术微型系统。 微流体二极管的特征在于简单的构造和柔性耦合能力，其中在经常的环境压力的范围内的静水压存在不同的微流系统。

    VERFAHREN UND VORRICHTUNG ZUR ÜBERTRAGUNG VON MIKRO- ODER NANOSTRUKTUREN DURCH KONTAKTSTEMPELN
    10.
    发明申请
    VERFAHREN UND VORRICHTUNG ZUR ÜBERTRAGUNG VON MIKRO- ODER NANOSTRUKTUREN DURCH KONTAKTSTEMPELN 审中-公开
    方法和装置用于转移微米或纳米结构通过以下方式联系TEMPLE

    公开(公告)号：WO2008154907A2

    公开(公告)日：2008-12-24

    申请号：PCT/DE2008/001002

    申请日：2008-06-23

    Applicant: GESIM GESELLSCHAFT FÜR SILIZIUM-MIKROSYSTEME MBH ,  HOWITZ, Steffen

    Inventor： HOWITZ, Steffen

    IPC: G03F7/00 ,  B41F17/00

    CPC classification number: B41F17/001 ,  B82Y10/00 ,  B82Y40/00 ,  G03F7/0002

    Abstract: Die Erfindung betrifft ein Verfahren und eine Vorrichtung zur Übertragung von Mikro- oder Nanostrukturen auf ein Substrat durch Kontaktstempeln. Der Stempel wird dabei aus einer Stempelaufnahme und einem Stempel unterteil gebildet, die lösbar miteinander verbunden sind. Dadurch wird sowohl ein schneller Wechsel zwischen verschiedenen Stempelunterteilen mit unterschiedlichen Mikro- oder Nanostrukturen möglich, als auch die Vermeidung mechanischer Belastung der Strukturmembranen erreicht. Die Übertragung der zu druckenden Materialien von der Quelle auf das Substrat erfolgt durch Benetzung der Strukturmembran mit den zu übertragenden Substanzen und der anschließenden Kontaktierung des Substrats von einem mittig auf der Strukturmembran befindlichen initialen Kontaktbereich nach außen konzentrisch anwachsend. Dadurch wird eine reproduzierbare Übertragung der Substanz erzielt. Die konvexe Verformung der Strukturmembran wird durch einen Überdruck in der aus der Stempelaufnahme und Strukturmembran gebildeten Kammer erreicht, wobei ein Gas oder eine Flüssigkeit über den Medienanschluss in die Kammer zugeführt wird. Bei permeabel gestalteter Strukturmembran besitzt die Kammer eine Reservoirfunktion für die zu übertragenden Materialien, die durch die Strukturmembran diffundieren. Durch einen Positionierungsmechanismus mit Markierungssystem werden Mikro- oder Nanoarrays mit verschiedenen Feinstrukturen auf der Substratoberfläche erzeugt.

    Abstract translation: 本发明涉及一种方法和用于通过接触柱塞转印微或纳米结构的衬底的装置。 印模从凸模夹持器和可拆卸地连接在一起的模具下部形成。 其特征在于两个具有不同的微米或纳米结构不同的RAM部件之间的快速变化是可能的，以及避免膜的结构的机械负荷得以实现。 打印材料从源到基板的转移是通过加湿用物质膜结构被转移并从位于中央的对膜结构的初始接触面积到外部的基片的后续接触同心增加进行。 其特征在于所述物质的可重现的传输来实现的。 结构膜的凸变形是由凸模夹持器和结构隔膜室形成的基团，其中气体或液体供给到腔室中的介质连接器中的过压来实现。 在设计的透过膜的结构，要传送具有用于材料的贮存功能的腔室，其通过膜结构扩散。 通过用标记系统微米或纳米阵列的定位机构与所述基片表面上不同的微观结构生成。

Patent Agency Ranking