摘要:
Provided are methods and systems for reference object assisted droplet detection, indexing, and sorting in assays and diagnostics. Provided are compositions of reference objects and methods of usages. Provided are systems and exemplary modules and functions for the detection, counting, indexing, data processing, and sorting of reference objects and assay droplets in a microfluidic device. The reference objects may serve as a dynamic micro-scale positioning system for the indexing and sorting of individual assay droplets.
摘要:
Disclosed are methods, devices and systems of an immunoassay using an alternate current electrokinetic platform. Also disclosed are methods of separating and detecting analytes from a sample using the disclosed methods.
摘要:
A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.
摘要:
A system for operating an electrokinetic device includes a support configured to hold and operatively couple with the electrokinetic device, an integrated electrical signal generation subsystem configured to apply a biasing voltage across a pair of electrodes in the electrokinetic device, and a light modulating subsystem configured to emit structured light onto the electrokinetic device. The system can further include a thermally controlled flow controller, and/or be configured to measure impedance across the electrokinetic device. The system can be a light microscope, including an optical train. The system can further include a light pipe, which can be part of the light modulating system, and which can be configured to supply light of substantially uniform intensity to the light modulating system or directly to the optical train.
摘要:
We describe a method comprising: providing a droplet comprising a plurality of constituents, splitting said droplet into a first droplet and a second droplet, wherein said first droplet comprises a first fraction of said plurality of constituents and said second droplet comprises a second fraction of said plurality of constituents, analysing said constituents of said first fraction of said plurality of constituents in said first droplet, and sorting said second droplet dependent on an outcome of said analysis.
摘要:
A microfluidic apparatus is provided that comprises a combination of dielectrophoresis (DEP) configurations each having an outer surface and electrowetting (EW) configurations each having an electrowetting surface. The DEP configurations can facilitate generating net DEP forces with respect to the outer surfaces of the DEP configurations to move micro-objects on the outer surfaces, and the EW configurations can facilitate changing wetting properties of the electrowetting surfaces to move droplets of liquid medium on the electrowetting surfaces. The EW configurations can be used to split a first part of a droplet of liquid medium from a second part of the droplet, while the DEP configurations can be used to move selected micro-objects into the first part of the droplet, before splitting of the droplet occurs.
摘要:
Provided herein is technology relating to depositing and/or placing a macromolecule at a desired site for an assay and particularly, but not exclusively, to methods and systems for placing or guiding a macromolecule such as a protein, a nucleic acid, or a protein:nucleic acid complex to an assay site, such as near a nanopore, a nanowell, or a zero mode waveguide.
摘要:
The present invention is notably directed to a microfluidic chip (1, 1a) comprising: a flow path (22) defined by a hydrophilic surface; a liquid input (24, 24a, 24b) on one side of the 4 flow path; at least one electrical circuit (62), hereafter DEP circuit, comprising at least one pair of dielectrophoretic electrodes (E21, E22), hereafter DEP electrodes, wherein: each of the DEP electrodes extends transverse to the flow path; and the DEP circuit is configured to generate a dielectrophoretic force, hereafter DEP force, at the level of the DEP electrodes. 8 The chip may further include one or more electroosmotic circuits. The present invention is further directed to methods of operation of such a microfluidic chip.
摘要:
Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.