公开(公告)号：WO2012106081A2

公开(公告)日：2012-08-09

申请号：PCT/US2012/021040

申请日：2012-01-12

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LTD. ,  KLAUSING, Kay ,  SHEN, Min-Jui Richard ,  MOORE, John ,  SMITH, Vincent ,  HALL, Kevin ,  GORMLEY, Niall Anthony ,  IOANNOU, Avgousta ,  FRITZILAS, Epameinondas ,  RIGATTI, Roberto

Inventor： KLAUSING, Kay ,  SHEN, Min-Jui Richard ,  MOORE, John ,  SMITH, Vincent ,  HALL, Kevin ,  GORMLEY, Niall Anthony ,  IOANNOU, Avgousta ,  FRITZILAS, Epameinondas ,  RIGATTI, Roberto

IPC: C12Q1/68 ,  C12Q1/48 ,  G01N33/53

CPC classification number: C12P19/34 ,  C12N15/1003 ,  C12Q1/6806 ,  C12Q2521/531 ,  C12Q2527/125 ,  C12Q2563/131

Abstract: Provided herein is a method of inhibiting degradation of nucleic acids during a nucleic acid processing step selected from fragmentation and detection comprising contacting the nucleic acids with a solution comprising gallic acid, analogues, derivatives thereof or mixtures thereof, during the processing step, wherein the contacting inhibits degradation of the nucleic acids. Also provided herein is a method of inhibiting light-induced degradation of nucleic acids. Additionally, provided herein is a method of reducing or inhibiting nucleic acid damage during preparation of a nucleic acid sample comprising fragmenting the nucleic acid sequences in the sample in a solution comprising one of more compounds, the compounds inhibiting degradation of the nucleic acid sequences in the sample.

Abstract translation: 本文提供了一种在选择片段化和检测的核酸加工步骤期间抑制核酸降解的方法，包括在加工步骤期间使核酸与包含没食子酸，其类似物，衍生物或其混合物的溶液接触，其中所述接触 抑制核酸的降解。 本文还提供抑制光诱导的核酸降解的方法。 此外，本文提供的是在制备核酸样品期间减少或抑制核酸损伤的方法，包括在包含一种或多种化合物的溶液中碎片样品中的核酸序列，所述化合物抑制核酸序列的降解 样品。

公开(公告)号：WO2016061517A2

公开(公告)日：2016-04-21

申请号：PCT/US2015/056040

申请日：2015-10-16

Applicant: ILLUMINA CAMBRIDGE LIMITED ,  STEEMERS, Frank J. ,  GUNDERSON, Kevin L. ,  ZHANG, Fan ,  BETLEY, Jason Richard ,  GORMLEY, Niall Anthony ,  MEULEMAN, Wouter ,  WEIR, Jacqueline ,  IOANNOU, Avgousta ,  JENKINS, Gareth ,  JACKSON, Rosamond ,  MORRELL, Natalie ,  POKHOLOK, Dmitry K. ,  NORBERG, Steven J. ,  HE, Molly ,  KIA, Amirali ,  GORYSHIN, Igor ,  PANTOJA, Rigo

Inventor： STEEMERS, Frank J. ,  GUNDERSON, Kevin L. ,  ZHANG, Fan ,  BETLEY, Jason Richard ,  GORMLEY, Niall Anthony ,  MEULEMAN, Wouter ,  WEIR, Jacqueline ,  IOANNOU, Avgousta ,  JENKINS, Gareth ,  JACKSON, Rosamond ,  MORRELL, Natalie ,  POKHOLOK, Dmitry K. ,  NORBERG, Steven J. ,  HE, Molly ,  KIA, Amirali ,  GORYSHIN, Igor ,  PANTOJA, Rigo

IPC: C12N15/10

CPC classification number: C12N15/1093 ,  C12N15/1065 ,  C12Q2525/191 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: Embodiments provided herein relate to methods and compositions for preparing an immobilized library of barcoded DNA fragments of a target nucleic acid, identifying genomic variants, determining the contiguity information, phasing information, and methylation status of the target nucleic acid.

Abstract translation: 本文提供的实施方案涉及用于制备靶核酸的条形码化DNA片段的固定文库，鉴定基因组变体，确定靶核酸的邻接信息，定相信息和甲基化状态的方法和组合物 酸

公开(公告)号：WO2016061517A3

公开(公告)日：2016-06-23

申请号：PCT/US2015056040

申请日：2015-10-16

Applicant: ILLUMINA CAMBRIDGE LTD ,  STEEMERS FRANK J ,  GUNDERSON KEVIN L ,  ZHANG FAN ,  BETLEY JASON RICHARD ,  GORMLEY NIALL ANTHONY ,  MEULEMAN WOUTER ,  WEIR JACQUELINE ,  IOANNOU AVGOUSTA ,  JENKINS GARETH ,  JACKSON ROSAMOND ,  MORRELL NATALIE ,  POKHOLOK DMITRY K ,  NORBERG STEVEN J ,  HE MOLLY ,  KIA AMIRALI ,  GORYSHIN IGOR ,  PANTOJA RIGO

Inventor： STEEMERS FRANK J ,  GUNDERSON KEVIN L ,  ZHANG FAN ,  BETLEY JASON RICHARD ,  GORMLEY NIALL ANTHONY ,  MEULEMAN WOUTER ,  WEIR JACQUELINE ,  IOANNOU AVGOUSTA ,  JENKINS GARETH ,  JACKSON ROSAMOND ,  MORRELL NATALIE ,  POKHOLOK DMITRY K ,  NORBERG STEVEN J ,  HE MOLLY ,  KIA AMIRALI ,  GORYSHIN IGOR ,  PANTOJA RIGO

IPC: C12N15/10

CPC classification number: C12N15/1093 ,  C12N15/1065 ,  C12Q2525/191 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: Embodiments provided herein relate to methods and compositions for preparing an immobilized library of barcoded DNA fragments of a target nucleic acid, identifying genomic variants, determining the contiguity information, phasing information, and methylation status of the target nucleic acid.

Abstract translation: 本文提供的实施方案涉及用于制备靶核酸的条形码DNA片段的固定化文库，鉴定基因组变体，确定目标核酸的邻接信息，定相信息和甲基化状态的方法和组合物。

公开(公告)号：WO2012106081A3

公开(公告)日：2012-11-01

申请号：PCT/US2012021040

申请日：2012-01-12

Applicant: ILLUMINA INC ,  ILLUMINA CAMBRIDGE LTD ,  KLAUSING KAY ,  SHEN MIN-JUI RICHARD ,  MOORE JOHN ,  SMITH VINCENT ,  HALL KEVIN ,  GORMLEY NIALL ANTHONY ,  IOANNOU AVGOUSTA ,  FRITZILAS EPAMEINONDAS ,  RIGATTI ROBERTO

Inventor： KLAUSING KAY ,  SHEN MIN-JUI RICHARD ,  MOORE JOHN ,  SMITH VINCENT ,  HALL KEVIN ,  GORMLEY NIALL ANTHONY ,  IOANNOU AVGOUSTA ,  FRITZILAS EPAMEINONDAS ,  RIGATTI ROBERTO

IPC: C12Q1/68 ,  C12Q1/48 ,  G01N33/53

CPC classification number: C12P19/34 ,  C12N15/1003 ,  C12Q1/6806 ,  C12Q2521/531 ,  C12Q2527/125 ,  C12Q2563/131

Abstract: Provided herein is a method of inhibiting degradation of nucleic acids during a nucleic acid processing step selected from fragmentation and detection comprising contacting the nucleic acids with a solution comprising gallic acid, analogues, derivatives thereof or mixtures thereof, during the processing step, wherein the contacting inhibits degradation of the nucleic acids. Also provided herein is a method of inhibiting light-induced degradation of nucleic acids. Additionally, provided herein is a method of reducing or inhibiting nucleic acid damage during preparation of a nucleic acid sample comprising fragmenting the nucleic acid sequences in the sample in a solution comprising one of more compounds, the compounds inhibiting degradation of the nucleic acid sequences in the sample.

Abstract translation: 本文提供了在选自片段化和检测的核酸加工步骤期间抑制核酸降解的方法，包括在加工步骤期间使核酸与包含没食子酸，其类似物，衍生物或其混合物的溶液接触，其中接触 抑制核酸的降解。 本文还提供了抑制核酸的光诱导降解的方法。 另外，本文提供了在制备核酸样品期间减少或抑制核酸损伤的方法，其包括在包含一种或多种化合物的溶液中使样品中的核酸序列片段化，所述化合物抑制核酸样品中核酸序列降解 样品。