公开(公告)号：WO2005040363A1

公开(公告)日：2005-05-06

申请号：PCT/GB2004/004330

申请日：2004-10-12

Applicant: THE UNIVERSITY OF YORK ,  BOWLES, Dianna ,  JACKSON, Rosamond ,  LIM, Eng-Kiat ,  VAISTIJ, Fabian

Inventor： BOWLES, Dianna ,  JACKSON, Rosamond ,  LIM, Eng-Kiat ,  VAISTIJ, Fabian

IPC: C12N5/10

CPC classification number: C12N9/1051 ,  C12N15/8255

Abstract: We describe transgenic cells which are transformed with nucleic acid molecules which encode glucosyltransferase polypeptides involved in phenylpropanoid biosynthesis.

Abstract translation: 我们描述用编码参与苯丙素生物合成的葡糖基转移酶多肽的核酸分子转化的转基因细胞。

公开(公告)号：WO2003023035A3

公开(公告)日：2003-03-20

申请号：PCT/GB2002/004143

申请日：2002-09-12

Applicant: THE UNIVERSITY OF YORK ,  JACKSON, Rosamond ,  LI, Yi ,  LIM, Eng-Kiat ,  BOWLES, Dianna, Joy

Inventor： JACKSON, Rosamond ,  LI, Yi ,  LIM, Eng-Kiat ,  BOWLES, Dianna, Joy

IPC: C12N15/54

Abstract: The invention relates to transgenic cells which have been transformed with glucosyltransferase nucleic acids which encode glucosyltransferases which glucosylate abscisic acid, or analogues thereof; the use of said glucosyltransferases in screens for agents with herbicidal activity and in the production and/or testing of abscisic acid, or analogues thereof.

公开(公告)号：WO2003023035A2

公开(公告)日：2003-03-20

申请号：PCT/GB2002/004143

申请日：2002-09-12

Applicant: THE UNIVERSITY OF YORK ,  JACKSON, Rosamond ,  LI, Yi ,  LIM, Eng-Kiat ,  BOWLES, Dianna, Joy

Inventor： JACKSON, Rosamond ,  LI, Yi ,  LIM, Eng-Kiat ,  BOWLES, Dianna, Joy

IPC: C12N15/54

CPC classification number: C12P19/44 ,  C12N9/1048 ,  C12N15/8293 ,  C12N2510/02 ,  C12Q1/48 ,  G01N33/5097 ,  G01N2333/91091 ,  G01N2500/04 ,  G01N2500/20

Abstract: The invention relates to transgenic cells which have been transformed with glucosyltransferase nucleic acids which encode glucosyltransferases which glucosylate abscisic acid, or analogues thereof; the use of said glucosyltransferases in screens for agents with herbicidal activity and in the production and/or testing of abscisic acid, or analogues thereof.

Abstract translation: 本发明涉及用葡糖基转移酶核酸转化的转基因细胞，所述葡糖基转移酶核酸编码使脱落酸葡糖基化的葡糖基转移酶或其类似物; 所述葡糖基转移酶在筛选具有除草活性的试剂和制备和/或测试脱落酸或其类似物中的用途。

公开(公告)号：WO2016061517A3

公开(公告)日：2016-06-23

申请号：PCT/US2015056040

申请日：2015-10-16

Applicant: ILLUMINA CAMBRIDGE LTD ,  STEEMERS FRANK J ,  GUNDERSON KEVIN L ,  ZHANG FAN ,  BETLEY JASON RICHARD ,  GORMLEY NIALL ANTHONY ,  MEULEMAN WOUTER ,  WEIR JACQUELINE ,  IOANNOU AVGOUSTA ,  JENKINS GARETH ,  JACKSON ROSAMOND ,  MORRELL NATALIE ,  POKHOLOK DMITRY K ,  NORBERG STEVEN J ,  HE MOLLY ,  KIA AMIRALI ,  GORYSHIN IGOR ,  PANTOJA RIGO

Inventor： STEEMERS FRANK J ,  GUNDERSON KEVIN L ,  ZHANG FAN ,  BETLEY JASON RICHARD ,  GORMLEY NIALL ANTHONY ,  MEULEMAN WOUTER ,  WEIR JACQUELINE ,  IOANNOU AVGOUSTA ,  JENKINS GARETH ,  JACKSON ROSAMOND ,  MORRELL NATALIE ,  POKHOLOK DMITRY K ,  NORBERG STEVEN J ,  HE MOLLY ,  KIA AMIRALI ,  GORYSHIN IGOR ,  PANTOJA RIGO

IPC: C12N15/10

CPC classification number: C12N15/1093 ,  C12N15/1065 ,  C12Q2525/191 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: Embodiments provided herein relate to methods and compositions for preparing an immobilized library of barcoded DNA fragments of a target nucleic acid, identifying genomic variants, determining the contiguity information, phasing information, and methylation status of the target nucleic acid.

Abstract translation: 本文提供的实施方案涉及用于制备靶核酸的条形码DNA片段的固定化文库，鉴定基因组变体，确定目标核酸的邻接信息，定相信息和甲基化状态的方法和组合物。

公开(公告)号：WO2013045939A1

公开(公告)日：2013-04-04

申请号：PCT/GB2012/052406

申请日：2012-09-28

Applicant: ILLUMINA, INC. ,  MEULEMAN, Wouter ,  CRESSINA, Elena ,  SMITH, Geoffrey Paul ,  JACKSON, Rosamond ,  LIU, Xiaohai

Inventor： MEULEMAN, Wouter ,  CRESSINA, Elena ,  SMITH, Geoffrey Paul ,  JACKSON, Rosamond ,  LIU, Xiaohai

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q2521/101 ,  C12Q2523/107 ,  C12Q2525/101 ,  C12Q2525/186 ,  C12Q2565/1015

Abstract: A reaction mixture including (a) a nucleic acid having a primer hybridized to a template, (b) nucleotide analogs, wherein the nucleotide analogs have a blocking moiety; (c) a polymerase that is capable of forming an extended primer by adding the nucleotide analogs to the primer, and (d) a deblocking agent that is capable of removing the blocking moiety from the extended primer. Also provided is a method of synthesizing a polynucleotide including sequentially adding a plurality of the different nucleotides analogs to the nucleic acid via several reaction cycles in the reaction mixture, wherein each reaction cycle includes (i) the polymerase adding a nucleotide analog to the nucleic acid to form a transient nucleic acid species comprising a blocking moiety, and (ii) the deblocking agent modifying the transient nucleic acid species to remove the blocking moiety.

Abstract translation: 一种反应混合物，其包含（a）具有与模板杂交的引物的核酸，（b）核苷酸类似物，其中所述核苷酸类似物具有阻断部分; （c）通过向引物中加入核苷酸类似物，能够形成延伸引物的聚合酶，和（d）能够从延伸引物除去阻断部分的解封剂。 还提供了合成多核苷酸的方法，所述多核苷酸包括在反应混合物中通过几个反应循环顺序加入多个不同的核苷酸类似物，其中每个反应循环包括（i）将核苷酸类似物加入到核酸中 以形成包含阻断部分的瞬时核酸物质，和（ii）修饰瞬态核酸物质以去除阻断部分的解封端剂。

公开(公告)号：WO2016061517A2

公开(公告)日：2016-04-21

申请号：PCT/US2015/056040

申请日：2015-10-16

Applicant: ILLUMINA CAMBRIDGE LIMITED ,  STEEMERS, Frank J. ,  GUNDERSON, Kevin L. ,  ZHANG, Fan ,  BETLEY, Jason Richard ,  GORMLEY, Niall Anthony ,  MEULEMAN, Wouter ,  WEIR, Jacqueline ,  IOANNOU, Avgousta ,  JENKINS, Gareth ,  JACKSON, Rosamond ,  MORRELL, Natalie ,  POKHOLOK, Dmitry K. ,  NORBERG, Steven J. ,  HE, Molly ,  KIA, Amirali ,  GORYSHIN, Igor ,  PANTOJA, Rigo

Inventor： STEEMERS, Frank J. ,  GUNDERSON, Kevin L. ,  ZHANG, Fan ,  BETLEY, Jason Richard ,  GORMLEY, Niall Anthony ,  MEULEMAN, Wouter ,  WEIR, Jacqueline ,  IOANNOU, Avgousta ,  JENKINS, Gareth ,  JACKSON, Rosamond ,  MORRELL, Natalie ,  POKHOLOK, Dmitry K. ,  NORBERG, Steven J. ,  HE, Molly ,  KIA, Amirali ,  GORYSHIN, Igor ,  PANTOJA, Rigo

IPC: C12N15/10

CPC classification number: C12N15/1093 ,  C12N15/1065 ,  C12Q2525/191 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: Embodiments provided herein relate to methods and compositions for preparing an immobilized library of barcoded DNA fragments of a target nucleic acid, identifying genomic variants, determining the contiguity information, phasing information, and methylation status of the target nucleic acid.

Abstract translation: 本文提供的实施方案涉及用于制备靶核酸的条形码化DNA片段的固定文库，鉴定基因组变体，确定靶核酸的邻接信息，定相信息和甲基化状态的方法和组合物 酸