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公开(公告)号：WO2015095407A2
公开(公告)日：2015-06-25
申请号：PCT/US2014/070979
申请日：2014-12-17
Applicant: LEWIS, Randolph V. , JONES, Justin A. , TUCKER, Chauncy L.
Inventor： LEWIS, Randolph V. , JONES, Justin A. , TUCKER, Chauncy L.
CPC classification number: B05D3/06 , A23V2002/00 , A61K8/0216 , A61K8/64 , A61K35/646 , A61K2800/10 , A61L27/227 , A61Q19/00 , B01D29/0093 , B29C35/08 , B29K2093/00 , B29L2007/00 , C08J5/18 , C08J2389/00 , G02B1/10 , Y10T428/31971 , Y10T442/20
Abstract: Methods for forming useful films using recombinant spider silk protein are discussed. In one embodiment, the method involves dissolving silk protein in a sufficient quantity to form a film suspended in a solvent. The solution is then mixed with a compound selected from water, acids, imidazole crosslinking agents, antibiotics, nanoparticles, surfactants and combinations thereof. Solubilisation of the protein in the solution is effected by microwave exposure. Once solubilized the microwave treated solution is poured onto a suitable substrate and dried in order to result in the final film, the protein may be plant, mammal or bacterially derived.
Abstract translation: 讨论了使用重组蜘蛛丝蛋白形成有用膜的方法。 在一个实施方案中，该方法包括将丝蛋白溶解在足够的量中以形成悬浮在溶剂中的膜。 然后将溶液与选自水，酸，咪唑交联剂，抗生素，纳米颗粒，表面活性剂及其组合的化合物混合。 溶液中蛋白质的溶解是通过微波暴露来实现的。 一旦溶解，将微波处理的溶液倒入合适的底物上并干燥以产生最终的膜，蛋白质可以是植物，哺乳动物或细菌来源的。
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公开(公告)号：WO2016164923A1
公开(公告)日：2016-10-13
申请号：PCT/US2016/026982
申请日：2016-04-11
Applicant: LEWIS, Randolph V. , JONES, Justin A , HASSOUNAH, Ibrahim , ABBOTT, Ethan J.
Inventor： LEWIS, Randolph V. , JONES, Justin A , HASSOUNAH, Ibrahim , ABBOTT, Ethan J.
IPC: C07K14/435 , D01F1/00 , D01F8/04 , D01F8/12 , D01F8/18
CPC classification number: D01D5/0038 , C08L33/06 , C08L33/20 , C08L61/02 , C08L67/00 , C08L69/00 , C08L71/02 , C08L75/04 , C08L77/02 , C08L77/04 , C08L77/06 , C08L79/02 , C08L89/00 , D01D10/02 , D01F6/90 , D01F6/96 , D02G1/0293 , D10B2211/04 , D10B2331/02 , D10B2331/10 , D10B2401/061 , D10B2401/063 , C08L29/04 , C08L31/04
Abstract: Spider silks and other natural silks are proteinaceous fibers composed largely of non-essential amino acids. Orb-web spinning spiders have as many as seven sets of highly specialized glands that produce up to seven different types of silk. Each silk protein has a different amino acid composition, mechanical property, and function. The physical properties of a silk fiber are influenced by the amino acid sequence, spinning mechanism, and environmental conditions in which they are produced. The present invention provides composition and methods for producing fibers comprising spider silk and synthetic polymers from electrospinning processes. Synthetic fiber blends and methods for preparing such fibers with spider silk proteins and synthetic and polymers are disclosed.
Abstract translation: 蜘蛛丝和其他天然丝是蛋白质纤维，主要由非必需氨基酸组成。 Orb-web旋转蜘蛛拥有多达七套高度专业的腺体，可生产七种不同类型的丝绸。 每种丝蛋白具有不同的氨基酸组成，机械性能和功能。 丝纤维的物理性质受氨基酸序列，纺丝机理和生产环境条件的影响。 本发明提供了由静电纺丝法制造包含蜘蛛丝和合成聚合物的纤维的组合物和方法。 公开了合成纤维混合物和用蜘蛛丝蛋白和合成和聚合物制备这种纤维的方法。
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公开(公告)号：WO2015023798A1
公开(公告)日：2015-02-19
申请号：PCT/US2014/050965
申请日：2014-08-13
Applicant: LEWIS, Randolph, V. , JONES, Justin, A.
Inventor： JONES, Justin, A.
IPC: D01F4/02
CPC classification number: B29D99/0078 , B29K2089/00 , B29L2031/731 , C07K14/43518 , C08H1/00 , C08L89/00 , C09D189/00 , D01F4/02
Abstract: A method for solubilizing recombinant spider silk proteins in an aqueous solutions, where the method includes mixing recombinant spider silk proteins with water to form a mixture and heating the mixture in a closed vessel to form a solution.
Abstract translation: 一种将重组蜘蛛丝蛋白溶解在水溶液中的方法，其中所述方法包括将重组蜘蛛丝蛋白与水混合以形成混合物并在密闭容器中加热混合物以形成溶液。
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公开(公告)号：WO2010033688A3
公开(公告)日：2010-03-25
申请号：PCT/US2009/057294
申请日：2009-09-17
Applicant: UNIVERSITY OF WYOMING , CHORESH, Omer , LEWIS, Randolph, V.
Inventor： CHORESH, Omer , LEWIS, Randolph, V.
IPC: C07K14/00
Abstract: Nucleic acids encoding spider glue proteins and methods of use thereof are disclosed.
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公开(公告)号：WO2002099082A2
公开(公告)日：2002-12-12
申请号：PCT/US2002/018256
申请日：2002-06-06
Applicant: UNIVERSITY OF WYOMING , ROTH, Donald, A. , LEWIS, Randolph, V.
Inventor： ROTH, Donald, A. , LEWIS, Randolph, V.
IPC: C12N
CPC classification number: C07K14/43518 , C12N15/8257
Abstract: The present invention relates to the fields of molecular biology and plant biology. Specifically, the invention is directed to the methods for expressing spider silk proteins in plants and the synthesis and purification of spider silk proteins therefrom.
Abstract translation: 本发明涉及分子生物学和植物生物学领域。 具体地说，本发明涉及在植物中表达蜘蛛丝蛋白的方法以及从其中合成和提纯蜘蛛丝蛋白。
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Patent Agency Ranking6.发明申请
公开(公告)号：WO2010033688A2
公开(公告)日：2010-03-25
申请号：PCT/US2009057294
申请日：2009-09-17
Applicant: UNIV WYOMING , CHORESH OMER , LEWIS RANDOLPH V
Inventor： CHORESH OMER , LEWIS RANDOLPH V
CPC classification number: C07K14/43518 , A61K38/00 , A61L24/108
Abstract: Nucleic acids encoding spider glue proteins and methods of use thereof are disclosed.
Abstract translation: 公开了编码蜘蛛胶蛋白的核酸及其使用方法。
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7.发明申请SPIDER SILK PROTEIN ENCODING NUCLEIC ACIDS, POLYPEPTIDES, ANTIBODIES AND METHOD OF USE THEREOF 审中-公开编码核酸的天蚕丝蛋白，多肽，抗体及其使用方法
公开(公告)号：WO03020916A8
公开(公告)日：2003-08-07
申请号：PCT/US0209663
申请日：2002-03-28
Applicant: UNIV WYOMING , LEWIS RANDOLPH V , HAYASHI CHERYL Y , GATESY JOHN E , MOTRIUK DAGMARA
Inventor： LEWIS RANDOLPH V , HAYASHI CHERYL Y , GATESY JOHN E , MOTRIUK DAGMARA
CPC classification number: C07K14/43518
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8.发明申请SPIDER SILK PROTEIN ENCODING NUCLEIC ACIDS, POLYPEPTIDES, ANTIBODIES AND METHOD OF USE THEREOF 审中-公开编码核酸的天蚕丝蛋白，多肽，抗体及其使用方法
公开(公告)号：WO2003020916A2
公开(公告)日：2003-03-13
申请号：PCT/US2002/009663
申请日：2002-03-28
Applicant: UNIVERSITY OF WYOMING , LEWIS, Randolph, V. , HAYASHI, Cheryl, Y. , GATESY, John, E. , MOTRIUK, Dagmara
Inventor： LEWIS, Randolph, V. , HAYASHI, Cheryl, Y. , GATESY, John, E. , MOTRIUK, Dagmara
IPC: C07H17/00
CPC classification number: C07K14/43518
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公开(公告)号：WO2016057851A1
公开(公告)日：2016-04-14
申请号：PCT/US2015/054782
申请日：2015-10-08
Applicant: LEWIS, Randolph V. , CHEN, Dong , JONES, Justin A. , KRISHNAJI, Sreevidhya T. , OLIVEIRA, Paula F. , BHATTACHARYYA, Gargi
Inventor： LEWIS, Randolph V. , CHEN, Dong , JONES, Justin A. , KRISHNAJI, Sreevidhya T. , OLIVEIRA, Paula F. , BHATTACHARYYA, Gargi
CPC classification number: C07K14/43563 , C07K14/43518 , C12N15/70
Abstract: A method of producing synthetic spider silk, including: transforming Escherichia coli with an expression vector; fermenting the transformed E. coli in a culture medium; inducing spider silk protein expression in the cultured E. coli; extracting the spider silk; and purifying the spider silk. Related vectors and genetically modified cells are also disclosed.
Abstract translation: 一种生产合成蜘蛛丝的方法，包括：用表达载体转化大肠杆菌; 在培养基中发酵转化的大肠杆菌; 在培养的大肠杆菌中诱导蜘蛛丝蛋白表达; 提取蜘蛛丝; 并净化蜘蛛丝。 还公开了相关载体和遗传修饰的细胞。
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公开(公告)号：WO02099082A3
公开(公告)日：2003-08-28
申请号：PCT/US0218256
申请日：2002-06-06
Applicant: UNIV WYOMING , ROTH DONALD A , LEWIS RANDOLPH V
Inventor： ROTH DONALD A , LEWIS RANDOLPH V
IPC: C07K14/435 , C12N15/82 , C12P21/06 , C07K14/00
CPC classification number: C07K14/43518 , C12N15/8257
Abstract: The present invention relates to the fields of molecular biology and plant biology. Specifically, the invention is directed to the methods for expressing spider silk proteins in plants and the synthesis and purification of spider silk proteins therefrom.
Abstract translation: 本发明涉及分子生物学和植物生物学领域。 具体地说，本发明涉及在植物中表达蜘蛛丝蛋白的方法以及从其中合成和提纯蜘蛛丝蛋白。
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