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    IN SITU HYBRIDIZATION DETECTION METHOD
    3.
    发明申请
    IN SITU HYBRIDIZATION DETECTION METHOD 审中-公开

    公开(公告)号：WO2007026252A3

    公开(公告)日：2007-03-08

    申请号：PCT/IB2006/003123

    申请日：2006-06-30

    Applicant: DAKO DENMARK A/S ,  WINTHER, Lars ,  LOHSE, Jesper ,  GABS, Susanne ,  PETERSEN, Kenneth, Heesche

    Inventor： WINTHER, Lars ,  LOHSE, Jesper ,  GABS, Susanne ,  PETERSEN, Kenneth, Heesche

    IPC: C12Q1/68

    Abstract: The invention provides compositions and methods for the detection of targets in a sample; in particular, an in situ hybridization (ISH) sample. Probes and detectable labels may be provided in multiple layers in order to increase the flexibility of a detection system, and to allow for amplification to enhance the signal from a target. The layers may be created by incorporating probes and detectable labels into larger molecular units that interact through nucleic acids base-pairing, including peptide-nucleic acid (PNA) base-pairing. Optional non-natural bases allow for degenerate base pairing schemes. The compositions and methods are also compatible with immunohistochemistry (IHC), immunocytochemistry (ICC), flow cytometry, enzyme immuno-assays (EIA), enzyme linked immuno-assays (ELISA), blotting methods (e.g. Western, Southern, and Northern), labeling inside electrophoresis systems or on surfaces or arrays, and precipitation, among other general detection assay formats. The invention is also compatible with many different types of targets, probes, and detectable labels.

    NEW METHOD FOR ENZYME-MEDIATED SIGNAL AMPLIFICATION
    5.
    发明申请
    NEW METHOD FOR ENZYME-MEDIATED SIGNAL AMPLIFICATION 审中-公开
    用于酶介导信号放大的新方法

    公开(公告)号：WO2012143010A1

    公开(公告)日：2012-10-26

    申请号：PCT/DK2012/000049

    申请日：2012-04-19

    Applicant: DAKO DENMARK A/S ,  LOHSE, Jesper ,  SKLADTCHIKOVA, Galina

    Inventor： LOHSE, Jesper ,  SKLADTCHIKOVA, Galina

    IPC: C12Q1/00

    CPC classification number: G01N33/581 ,  C12Q1/26 ,  C12Q1/28 ,  G01N33/57492

    Abstract: The present invention relates to methods and compounds for enzyme-mediated amplification of target-associated signals for visualization of biological or chemical targets in samples, wherein the targets are immobilized, in particular the invention relates to the oxidoreductase-mediated signal amplification for visualization of targets in samples comprising cells. The methods of the invention are particular useful for qualitative and quantitative evaluation of biological markers relating to medical diagnostic and therapy.

    Abstract translation: 本发明涉及用于酶介导扩增靶标相关信号的方法和化合物，用于在样品中生物或化学靶标的可视化，其中靶标被固定，特别是本发明涉及用于目标可视化的氧化还原酶介导的信号放大 在包含细胞的样品中。 本发明的方法对于与医学诊断和治疗有关的生物学标记的定性和定量评估特别有用。

    QUANTIFICATION OF SINGLE TARGET MOLECULES IN HISTOLOGICAL SAMPLES
    7.
    发明申请
    QUANTIFICATION OF SINGLE TARGET MOLECULES IN HISTOLOGICAL SAMPLES 审中-公开
    定量分析样本中的单目标分子

    公开(公告)号：WO2012062318A1

    公开(公告)日：2012-05-18

    申请号：PCT/DK2011/000131

    申请日：2011-11-08

    Applicant: DAKO DENMARK A/S ,  LOHSE, Jesper ,  SKLADTCHIKOVA, Galina

    Inventor： LOHSE, Jesper ,  SKLADTCHIKOVA, Galina

    IPC: G01N33/53 ,  G01N33/58

    CPC classification number: G01N33/543 ,  G01N33/5088 ,  G01N33/532 ,  G01N33/58 ,  G01N33/6893 ,  G01N2800/50

    Abstract: The present invention lies in the field of visualization and quantification of immobilized targets in samples using immunochemical means. The methods of the invention utilize an immunostaining system allowing visualizing single target units in samples as distinct dots. In particular, the invention relates to methods and reagents for visualization and quantification of molecular targets immunostained in histological samples and use of said method and reagents in medical diagnostic. However, the visualization and quantification methods of the invention are applicable to a variety of targets in different samples and allow precise quantifying both relative and absolute amounts thereof.

    Abstract translation: 本发明在于使用免疫化学方法在样品中固定化靶标的可视化和定量的领域。 本发明的方法利用免疫染色系统，允许将样品中的单个靶单元可视化为不同的点。 特别地，本发明涉及用于在组织学样品中免疫染色的分子靶标的可视化和定量的方法和试剂，以及在医学诊断中使用所述方法和试剂。 然而，本发明的可视化和定量方法适用于不同样品中的各种靶标，并允许精确量化其相对和绝对量。

    METHODS AND COMPOUNDS FOR DETECTION OF MOLECULAR TARGETS
    8.
    发明申请
    METHODS AND COMPOUNDS FOR DETECTION OF MOLECULAR TARGETS 审中-公开
    用于检测分子靶标的方法和化合物

    公开(公告)号：WO2010094284A1

    公开(公告)日：2010-08-26

    申请号：PCT/DK2010/000022

    申请日：2010-02-19

    Applicant: DAKO DENMARK A/S ,  LOHSE, Jesper

    Inventor： LOHSE, Jesper

    IPC: C12Q1/28 ,  C08G63/52

    CPC classification number: G01N33/54393 ,  C08G63/672 ,  C08G69/40 ,  C08G69/48 ,  C12Q1/28

    Abstract: The methods involve detection of peroxidase activity of one or more binding agents comprising peroxidase activity and a compound comprising two or more moieties capable of serving as substrate for a peroxidase enzyme, one or more detectable labels and a water soluble polymer, wherein said two or more moieties capable of serving as substrate for a peroxidase enzyme and said one or more labels are linked to said polymer so that a distance separating any of the linked moieties from any of the linked labels is at least 30 consecutively interconnected atoms, and wherein a distance separating any two of the two or more linked moieties capable of serving as substrate for a peroxidase enzyme is less than 30 consecutively interconnected atoms. In the reaction the conjugate is deposited.

    Abstract translation: 所述方法涉及检测一种或多种包含过氧化物酶活性的结合剂的过氧化物酶活性和包含两个或多个能够用作过氧化物酶的底物的部分，一种或多种可检测标记和水溶性聚合物的化合物，其中所述两种或多种 能够用作过氧化物酶的底物的部分和所述一个或多个标记连接到所述聚合物，使得将任何连接的部分与任何连接的标记物分开的距离为至少30个连续相互连接的原子，并且其中分离的距离 能够作为过氧化物酶的底物的两个或更多个连接部分中的任何两个小于30个连续相互连接的原子。 在反应中，共轭物被沉积。

    CONJUGATE MOLECULES
    9.
    发明申请
    CONJUGATE MOLECULES 审中-公开
    结合分子

    公开(公告)号：WO2010094283A1

    公开(公告)日：2010-08-26

    申请号：PCT/DK2010/000021

    申请日：2010-02-19

    Applicant: DAKO DENMARK A/S ,  LOHSE, Jesper

    Inventor： LOHSE, Jesper

    IPC: C12Q1/28 ,  C08G63/52

    CPC classification number: G01N33/54393 ,  C08G63/672 ,  C08G69/40 ,  C08G69/48 ,  C12Q1/28

    Abstract: The present invention relates to compounds which are conjugates of two or more moieties capable of serving as substrates of an enzyme with peorxidase activity and one or more labels. The conjugate molecules of the invention are useful for detection of molecular targets, e.g. biological or chemical molecules, molecular structures, etc, in samples using a host of experimental schemes for detecting and visualizing such targets, e.g. immunohistochemistry (IHC), in situ hybridization (ISH), ELlSA, Southern, Northern, and Western blotting, etc. The invention also relates to compositions comprising the conjugate molecules. The labels and moieties of peroxidase substrate are not the same chemical substances and are linked together via a linker, wherein said linker is a compound comprising at least one linear chain of at least 30 consecutively connected atoms, wherein said linear chain contain at least two branching points separated by a distance of at least 30 consecutevely connected atoms and the labels and peroxidase substrate moieties are separated in the conjugate molecule by least 30 consecutively connected atoms and two neighboring of the peroxidase substrate moieties are separated from each other by less than 30 consecutively connected atoms.

    Abstract translation: 本发明涉及作为能够作为具有一种或多种标记的酶的底物的两个或更多个部分的缀合物的化合物。 本发明的共轭分子可用于检测分子靶，例如 生物或化学分子，分子结构等，使用大量用于检测和可视化这些靶的实验方案，例如， 免疫组化（IHC），原位杂交（ISH），ELLSA，Southern，Northern和Western印迹等。本发明还涉及包含缀合物分子的组合物。 过氧化物酶底物的标记和部分不是相同的化学物质，并且通过接头连接在一起，其中所述接头是包含至少一个至少30个连续原子的直链的化合物，其中所述直链含有至少两个支链 与至少30个连续连接的原子间隔开的点和标记物和过氧化物酶底物部分在缀合物分子中由至少30个连续连接的原子分离，并且两个相邻的过氧化物酶底物部分彼此分开小于30个连续连接 原子。

    IN SITU HYBRIDIZATION DETECTION METHOD
    10.
    发明申请
    IN SITU HYBRIDIZATION DETECTION METHOD 审中-公开
    原位杂交检测方法

    公开(公告)号：WO2007026252A2

    公开(公告)日：2007-03-08

    申请号：PCT/IB2006003123

    申请日：2006-06-30

    Applicant: DAKO DENMARK AS ,  WINTHER LARS ,  LOHSE JESPER ,  GABS SUSANNE ,  PETERSEN KENNETH HEESCHE

    Inventor： WINTHER LARS ,  LOHSE JESPER ,  GABS SUSANNE ,  PETERSEN KENNETH HEESCHE

    CPC classification number: C12Q1/6804 ,  A61K47/61 ,  A61K47/6843 ,  A61K47/6889 ,  A61K49/0041 ,  A61K49/0043 ,  A61K49/0056 ,  C07C231/02 ,  C07C233/36 ,  C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C08G69/02 ,  C12Q1/6876 ,  G01N33/5306

    Abstract: The invention provides compositions and methods for the detection of targets in a sample; in particular, an in situ hybridization (ISH) sample. Probes and detectable labels may be provided in multiple layers in order to increase the flexibility of a detection system, and to allow for amplification to enhance the signal from a target. The layers may be created by incorporating probes and detectable labels into larger molecular units that interact through nucleic acids base-pairing, including peptide-nucleic acid (PNA) base-pairing. Optional non-natural bases allow for degenerate base pairing schemes. The compositions and methods are also compatible with immunohistochemistry (IHC), immunocytochemistry (ICC), flow cytometry, enzyme immuno-assays (EIA), enzyme linked immuno-assays (ELISA), blotting methods (e.g. Western, Southern, and Northern), labeling inside electrophoresis systems or on surfaces or arrays, and precipitation, among other general detection assay formats. The invention is also compatible with many different types of targets, probes, and detectable labels.

    Abstract translation: 本发明提供用于检测样品中靶标的组合物和方法; 特别是原位杂交（ISH）样品。 可以以多层提供探针和可检测标记，以便增加检测系统的灵活性，并允许放大以增强来自靶的信号。 可以通过将探针和可检测标记掺入通过核酸碱基配对（包括肽 - 核酸（PNA）碱基配对）相互作用的较大分子单元中来产生层。 可选的非天然碱基允许简并碱基配对方案。 组合物和方法还与免疫组织化学（IHC），免疫细胞化学（ICC），流式细胞术，酶免疫测定（EIA），酶联免疫测定（ELISA），印迹方法（例如西方，南方和北方）相容， 标记电泳系统或表面或阵列，以及其他一般检测测定形式的沉淀。 本发明还与许多​​不同类型的靶，探针和可检测标记相兼容。

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