公开(公告)号：WO2013057308A2

公开(公告)日：2013-04-25

申请号：PCT/EP2012070856

申请日：2012-10-22

Applicant: QIAGEN INSTR AG

Inventor： LEBER DIRK ,  BAEHLER STEPHAN ,  MEYER HANSUELI

IPC: G01K11/20 ,  G01K11/16 ,  G01K15/00

CPC classification number: G01K15/007 ,  G01K11/165 ,  G01K11/20 ,  G01K15/005

Abstract: The present invention pertains to a method suitable for analysing the temperature control of a device which is supposed to establish a defined temperature in a micro-environment, said method comprising a first Optical Temperature Verification step which comprises a) providing one or more thermochromatic liquid crystals in a micro-environment, wherein each thermochromatic liquid crystal has a specific event temperature; b) providing one or more temperature dependent luminophores in a micro-environment; c) varying the temperature in the micro-environments and irradiating the micro-environments with light; d) recording the luminescence of the one or more temperature dependent luminophores when the event temperature of the one or more thermochromatic liquid crystals is reached in the micro-environment; and wherein said method preferably comprises a second Optical Temperature Verification step, which comprises the following: a) providing one or more temperature dependent luminophores that were used in the First Optical Temperature Verification in a micro-environment; b) varying the temperature of the micro-environments and irradiating the micro-environments with light; c) monitoring the luminescence emitted. The respective method is very suitable to analyse the temperature control of a thermal cycler, in particular during a calibration.

Abstract translation: 本发明涉及一种适用于分析假定在微环境中建立限定温度的装置的温度控制的方法，所述方法包括第一光学温度验证步骤，该步骤包括：a）提供一个或多个热色液晶 在微环境中，其中每个热色液晶具有特定的事件温度; b）在微环境中提供一个或多个温度依赖发光体; c）改变微环境中的温度并用光照射微环境; d）当在微环境中达到一种或多种热色液晶的事件温度时，记录一个或多个温度依赖发光体的发光; 并且其中所述方法优选地包括第二光学温度验证步骤，其包括以下步骤：a）提供在微环境中在第一光学温度校验中使用的一个或多个与温度相关的发光体; b）改变微环境的温度并用光照射微环境; c）监测发射的发光。 相应的方法非常适合分析热循环仪的温度控制，特别是在校准过程中。

公开(公告)号：WO2017037535A3

公开(公告)日：2017-04-20

申请号：PCT/IB2016001318

申请日：2016-08-31

Applicant: QIAGEN INSTR AG

Inventor： QUINTEL HARALD ,  LUTZE KONSTANTIN

IPC: G01J3/51 ,  C12Q1/68 ,  G01J3/10 ,  G01J3/36

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  G01J3/10 ,  G01J3/2823 ,  G01J3/36 ,  G01J3/513 ,  G01J2003/2826 ,  G02B5/201 ,  G02B26/0875 ,  G02B26/0883 ,  G02B27/141 ,  H04N5/2254 ,  H04N5/2256 ,  H04N5/332 ,  H04N9/045 ,  C12Q2523/313 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2563/107 ,  C12Q2565/102

Abstract: A sequencing instrument optical system having a combined light source with multiple collinear excitation beams having different respective excitation wavelengths, a sequencing surface having DNA templates and nucleobase labels configured to emit a respective emission light at a different respective emission wavelength upon excitation by one or more of the excitation beams, a color camera configured to detect the emission light of each of the nucleobase labels, a first optical pathway configured to direct the collinear excitation beams from the combined light source to the sequencing surface, and a second optical pathway configured to direct the emission light from the sequencing surface to the color camera.

Abstract translation: 一种测序仪器光学系统，其具有组合光源和多个共线激发光束，所述多个共线激发光束具有不同的相应激发波长，测序表面具有DNA模板和核碱基标签，所述DNA模板和核碱基标签被配置为在受到以下一项或多项的激发时发射各自的发射光： 激发光束，被配置为检测每个核碱基标签的发射光的彩色照相机，被配置为将来自组合光源的共线激发光束引导到序列表面的第一光学路径，以及被配置为引导 从排序表面发射光线到彩色相机。

公开(公告)号：WO2009105030A2

公开(公告)日：2009-08-27

申请号：PCT/SE2009050198

申请日：2009-02-23

Applicant: QIAGEN INSTR AG ,  SMITH DAVID ,  HAGERLID PETER

Inventor： SMITH DAVID ,  HAGERLID PETER

IPC: B01L3/00

CPC classification number: B29C59/002 ,  B01L3/5085 ,  B01L2200/141 ,  B01L2300/0829 ,  B01L2300/168

Abstract: The present invention relates to a microtitre plate with wells having transparent bottoms, wherein said microtitre plate comprises at least one physical deformation between at least two adjacent wells. The physical deformations may have the shapeof e.g. a channel, a ridge, a hole, a slit or a step.

Abstract translation: 本发明涉及具有透明底部孔的微量滴定板，其中所述微量滴定板包括至少两个相邻孔之间的至少一个物理变形。 物理变形可能具有如下特征。 通道，脊，孔，狭缝或台阶。