公开(公告)号：WO2012035508A4

公开(公告)日：2012-07-19

申请号：PCT/IB2011054035

申请日：2011-09-15

Applicant: SPINOMIX S A ,  RIDA AMAR ,  MERMOD NICOLAS ,  FRANCOIS PATRICE ,  LAZAREVIC VLADIMIR ,  SCHRENZEL JACQUES

Inventor： RIDA AMAR ,  MERMOD NICOLAS ,  FRANCOIS PATRICE ,  LAZAREVIC VLADIMIR ,  SCHRENZEL JACQUES

IPC: C12Q1/04 ,  C12Q1/14 ,  C12Q1/56 ,  G01N33/569 ,  G01N33/86

CPC classification number: G01N33/86 ,  C12Q1/56 ,  G01N33/56938 ,  G01N2333/75 ,  G01N2333/974

Abstract: A method for separating target molecules or particles from a fibrinogen containing sample comprises: (a) trapping the said target molecules or particles in a fibrin network by converting at least partially the fibrinogen contained in the sample into fibrin; (b) retracting the said fibrin network to form a fibrin clot; (c) separating the said fibrin clot from the surrounding sample medium,

Abstract translation: 从含有纤维蛋白原的样品中分离靶分子或颗粒的方法包括：（a）将至少部分地将样品中所含的纤维蛋白原转化成纤维蛋白，将所述靶分子或颗粒捕获在纤维蛋白网络中; （b）缩回所述纤维蛋白网络以形成纤维蛋白凝块; （c）将所述纤维蛋白凝块与周围的样品介质分离，

公开(公告)号：WO2012035508A2

公开(公告)日：2012-03-22

申请号：PCT/IB2011/054035

申请日：2011-09-15

Applicant: SPINOMIX S.A. ,  RIDA, Amar ,  MERMOD, Nicolas ,  FRANCOIS, Patrice ,  LAZAREVIC, Vladimir ,  SCHRENZEL, Jacques

Inventor： RIDA, Amar ,  MERMOD, Nicolas ,  FRANCOIS, Patrice ,  LAZAREVIC, Vladimir ,  SCHRENZEL, Jacques

IPC: G01N33/86

CPC classification number: G01N33/86 ,  C12Q1/56 ,  G01N33/56938 ,  G01N2333/75 ,  G01N2333/974

Abstract: A method for separating target molecules or particles from a fibrinogen containing sample comprises: (a) trapping the said target molecules or particles in a fibrin network by converting at least partially the fibrinogen contained in the sample into fibrin; (b) retracting the said fibrin network to form a fibrin clot; (c) separating the said fibrin clot from the surrounding sample medium,

Abstract translation: 用于从含有纤维蛋白原的样品中分离目标分子或颗粒的方法包括：（a）通过将所述样品中所含的纤维蛋白原至少部分转变成纤维蛋白，将所述目标分子或颗粒捕获在纤维蛋白网络中 ; （b）回缩所述纤维蛋白网络以形成纤维蛋白凝块; （c）将所述纤维蛋白凝块与周围的样品介质分开，

公开(公告)号：WO2008116543A1

公开(公告)日：2008-10-02

申请号：PCT/EP2008/001706

申请日：2008-03-04

Applicant: F. HOFFMANN-LA ROCHE AG ,  ROCHE DIAGNOSTICS GMBH ,  ELSENHANS, Olivier ,  SAVATIC, Goran ,  GIJS, Martin ,  RIDA, Amar ,  FERNANDEZ, Victor

Inventor： ELSENHANS, Olivier ,  SAVATIC, Goran ,  GIJS, Martin ,  RIDA, Amar ,  FERNANDEZ, Victor

IPC: B03C1/253

CPC classification number: B03C1/253 ,  B03C1/0335 ,  B03C1/288 ,  B03C2201/18

Abstract: A method and an apparatus for transporting magnetic or magnetisable microbeads immersed in a liquid contained in a capillary tube having a length symmetry axis which defines an axial direction, the transporting being effected in the absence of a static magnetic field in the capillary tube.

Abstract translation: 一种用于输送浸入包含在限定轴向的长度对称轴的毛细管中的液体中的磁性或可磁化微珠的方法和装置，该传输是在毛细管中没有静磁场的情况下进行的。

公开(公告)号：WO2006056579A3

公开(公告)日：2006-06-01

申请号：PCT/EP2005/056178

申请日：2005-11-23

Applicant: SPINOMIX S.A. ,  RIDA, Amar

Inventor： RIDA, Amar

IPC: G01N33/543 ,  H01F1/00

Abstract: Bio-functionalized magnetic particles have a non-magnetic material matrix which supports core/shell magnetic elements composed from a ferromagnetic core material and a shell material. The shell material can be chosen either among an antiferromagnetic material, a ferromagnetic material of a kind different from the core ferromagnetic material or a metal material. By a proper choice of materials and dimension tuning of both the core and the shell as well as the amount and the concentration of the magnetic elements within the non- magnetic matrix, the bio-functionalized magnetic particles is tailored to exhibit an enhanced magnetic energy. When subjected to an alternating magnetic field, the magnetic particles exhibit specific rotational dynamics in correspondence with the amplitude and the frequency of the applied magnetic filed. Aggregation structures of the magnetic particles are controlled and manipulated by the alternating magnetic field. The magnetic particles are functionalized by specific ligands for the probing and manipulating of biomolecules and chemical substances.

公开(公告)号：WO2008007270A3

公开(公告)日：2008-04-17

申请号：PCT/IB2007052409

申请日：2007-06-21

Applicant: SPINOMIX S A ,  RIDA AMAR

Inventor： RIDA AMAR

IPC: H01F1/44 ,  B22F9/02 ,  G01N33/553 ,  H01F1/28

CPC classification number: B01F13/0809 ,  B01F13/0059 ,  B82Y5/00 ,  B82Y25/00 ,  H01F1/0054

Abstract: A method of mixing magnetic particles (3) in a reaction chamber (2) that is part of a microfluidic device and that contains the said particles in suspension, comprises the steps: (a) providing an electromagnetic means (1,1 ',6, 7) to generate magnetic field sequences having polarity and intensity that vary in time and a magnetic field gradient that covers the whole space of the said reaction chamber (2); (b) applying a first magnetic field sequence to separate or confine the particles (3) so the particles occupy a sub-volume in the volume of the reaction chamber (2); (c) injecting a defined volume of the said reagent in the reaction chamber; and (d) applying a second magnetic field sequence to leads the particles (3) to be homogenously distributed and dynamically moving over a substantial portion of the whole reaction chamber volume.

Abstract translation: 在作为微流体装置的一部分并且包含所述悬浮液的颗粒的反应室（2）中混合磁性颗粒（3）的方法包括以下步骤：（a）提供电磁装置（1,1'，6 ，7）产生具有时间上变化的极性和强度的磁场序列和覆盖所述反应室（2）的整个空间的磁场梯度; （b）施加第一磁场序列以分离或限制颗粒（3），使得颗粒占据反应室（2）的体积中的子体积; （c）将限定体积的所述试剂注入反应室中; 和（d）施加第二磁场序列以使颗粒（3）均匀分布并在整个反应室体积的大部分上动态移动。

公开(公告)号：WO2008007270A2

公开(公告)日：2008-01-17

申请号：PCT/IB2007/052409

申请日：2007-06-21

Applicant: SPINOMIX S.A. ,  RIDA, Amar

Inventor： RIDA, Amar

CPC classification number: B01F13/0809 ,  B01F13/0059 ,  B82Y5/00 ,  B82Y25/00 ,  H01F1/0054

Abstract: A method of mixing magnetic particles (3) in a reaction chamber (2) that is part of a microfluidic device and that contains the said particles in suspension, comprises the steps: (a) providing an electromagnetic means (1,1 ',6, 7) to generate magnetic field sequences having polarity and intensity that vary in time and a magnetic field gradient that covers the whole space of the said reaction chamber (2); (b) applying a first magnetic field sequence to separate or confine the particles (3) so the particles occupy a sub-volume in the volume of the reaction chamber (2); (c) injecting a defined volume of the said reagent in the reaction chamber; and (d) applying a second magnetic field sequence to leads the particles (3) to be homogenously distributed and dynamically moving over a substantial portion of the whole reaction chamber volume.

Abstract translation: 一种在作为微流体装置的一部分的反应室（2）中混合磁性颗粒（3）并且包含处于悬浮液中的所述颗粒的方法包括以下步骤：（a）提供电磁 用于产生磁场序列的装置（1,1'，6,7），所述磁场序列具有随时间变化的极性和强度以及覆盖所述反应室（2）的整个空间的磁场梯度; （b）施加第一磁场序列以分离或限制粒子（3），使得粒子占据反应室（2）的体积中的子体积; （c）将预定体积的所述试剂注入反应室中; （d）施加第二磁场序列以引导颗粒（3）均匀分布并在整个反应室体积的大部分上动态移动。

公开(公告)号：WO2006056579A2

公开(公告)日：2006-06-01

申请号：PCT/EP2005056178

申请日：2005-11-23

Applicant: SPINOMIX S A ,  RIDA AMAR

Inventor： RIDA AMAR

IPC: G01N33/543 ,  H01F1/00

CPC classification number: G01N33/5434 ,  Y10T428/2991

Abstract: Bio-functionalized magnetic particles have a non-magnetic material matrix which supports core/shell magnetic elements composed from a ferromagnetic core material and a shell material. The shell material can be chosen either among an antiferromagnetic material, a ferromagnetic material of a kind different from the core ferromagnetic material or a metal material. By a proper choice of materials and dimension tuning of both the core and the shell as well as the amount and the concentration of the magnetic elements within the non- magnetic matrix, the bio-functionalized magnetic particles is tailored to exhibit an enhanced magnetic energy. When subjected to an alternating magnetic field, the magnetic particles exhibit specific rotational dynamics in correspondence with the amplitude and the frequency of the applied magnetic filed. Aggregation structures of the magnetic particles are controlled and manipulated by the alternating magnetic field. The magnetic particles are functionalized by specific ligands for the probing and manipulating of biomolecules and chemical substances.

Abstract translation: 生物功能化磁性颗粒具有非磁性材料基质，该基质支撑由铁磁芯材料和壳材料组成的核/壳磁性元件。 壳材料可以选自反铁磁材料，不同于铁心铁磁材料的铁磁材料或金属材料。 通过适当选择核心和壳体的材料和尺寸调整以及非磁性基质内的磁性元素的量和浓度，生物功能化的磁性颗粒被调整以显示增强的磁能。 当受到交变磁场时，磁性颗粒表现出与施加的磁场的幅度和频率相对应的特定旋转动力学。 磁性粒子的聚集结构受交变磁场控制和操纵。 磁性颗粒被特定的配体官能化，用于探测和操作生物分子和化学物质。

公开(公告)号：WO2012035508A3

公开(公告)日：2012-05-18

申请号：PCT/IB2011054035

申请日：2011-09-15

Applicant: SPINOMIX S A ,  RIDA AMAR ,  MERMOD NICOLAS ,  FRANCOIS PATRICE ,  LAZAREVIC VLADIMIR ,  SCHRENZEL JACQUES

Inventor： RIDA AMAR ,  MERMOD NICOLAS ,  FRANCOIS PATRICE ,  LAZAREVIC VLADIMIR ,  SCHRENZEL JACQUES

IPC: C12Q1/04 ,  C12Q1/14 ,  C12Q1/56 ,  G01N33/569 ,  G01N33/86

CPC classification number: G01N33/86 ,  C12Q1/56 ,  G01N33/56938 ,  G01N2333/75 ,  G01N2333/974

Abstract: A method for separating target molecules or particles from a fibrinogen containing sample comprises: (a) trapping the said target molecules or particles in a fibrin network by converting at least partially the fibrinogen contained in the sample into fibrin; (b) retracting the said fibrin network to form a fibrin clot; (c) separating the said fibrin clot from the surrounding sample medium,

Abstract translation: 从含有纤维蛋白原的样品中分离目标分子或颗粒的方法包括：（a）通过将样品中所含的纤维蛋白原至少部分转化为纤维蛋白，将所述目标分子或颗粒捕获在纤维蛋白网络中; （b）回缩所述纤维蛋白网络以形成纤维蛋白凝块; （c）将所述纤维蛋白凝块与周围的样品介质分开，

公开(公告)号：WO2008010111A2

公开(公告)日：2008-01-24

申请号：PCT/IB2007/052410

申请日：2007-06-21

Applicant: SPINOMIX S.A. ,  RIDA, Amar

Inventor： RIDA, Amar

CPC classification number: B01F13/0809 ,  B01F13/0059 ,  B82Y5/00 ,  B82Y25/00 ,  H01F1/0054

Abstract: A device for manipulating and mixing magnetic particles (3) in a surrounding liquid medium, comprises at least one couple of magnetic poles (1,1') facing each other across a gap, the facing poles diverging from a narrow end of the gap to a large end of the gap, the poles (1,1') forming part of an electromagnetic circuit and being arranged to provide a magnetic field gradient in the gap region; and a reaction chamber (2) that is a part of a fluidic network for containing the said magnetic particles in suspension and placed in the gap of the said electromagnets poles (1,1'). The reaction chamber (2) preferably has at least one part which has a diverging cavity, arranged co-divergently in the diverging gap between the poles.

Abstract translation: 用于在周围液体介质中操纵和混合磁性颗粒（3）的装置包括至少一对磁极（1,1'），所述磁极跨越间隙彼此面对，所述相对磁极发散 从所述间隙的窄端到所述间隙的大端，所述极（1,1'）形成电磁电路的一部分并被布置为在所述间隙区域中提供磁场梯度; 和作为流体网络的一部分的反应室（2），用于容纳悬浮的所述磁性颗粒并放置在所述电磁体磁极（1,1'）的间隙中。 反应室（2）优选具有至少一个具有发散空腔的部分，该发散空腔在两极之间的发散间隙中共同地分散布置。

公开(公告)号：WO2004078316A1

公开(公告)日：2004-09-16

申请号：PCT/IB2003/000956

申请日：2003-03-08

Applicant: ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL) ,  FERNANDEZ, Victor ,  RIDA, Amar ,  GIJS, Martin

Inventor： FERNANDEZ, Victor ,  RIDA, Amar ,  GIJS, Martin

IPC: B01D35/06

CPC classification number: B03C1/035 ,  B01F13/0059 ,  B01F13/0818 ,  B01F2215/0037 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2400/0406 ,  B01L2400/043 ,  B03C1/288 ,  G01N27/745 ,  G01N35/0098 ,  G01N2035/00574 ,  Y10T436/2575

Abstract: A device for transporting magnetic or magnetisable microbeads (25) in a capillary chamber (14) comprises a permanent magnet (10) or an electromagnet (11) for subjecting the capillary chamber to a substantially uniform magnetic field, to apply a permanent magnetic moment to the microbeads (25). At least one planar coil (22) and preferably an array of overlapping coils are located adjacent to the capillary chamber (14) for applying a complementary magnetic field on the microbeads parallel or antiparallel to said substantially uniform magnetic field, to drive the microbeads. An arrangement is provided for switching the current applied to the coil(s) (22) to invert the field produced thereby, to selectively apply an attractive or repulsive driving force on the microbeads (25). The device is usable to transport microbeads for performing chemical and biochemical reactions or assay, as is done for instance in clinical chemistry assays for medical diagnostic purposes.

Abstract translation: 用于在毛细管室（14）中传送磁性或可磁化微珠（25）的装置包括永磁体（10）或电磁体（11），用于对毛细管腔进行基本均匀的磁场，以将永久磁矩施加到 微珠（25）。 至少一个平面线圈（22）并且优选地是重叠线圈阵列位于毛细管腔（14）附近，用于在平行或反平行于所述基本上均匀的磁场的微珠上施加互补磁场，以驱动微珠。 提供一种用于切换施加到线圈（22）的电流以反转由此产生的场的布置，以选择性地在微珠（25）上施加有吸引力或排斥力的驱动力。 该装置可用于运输微珠进行化学和生物化学反应或测定，如在用于医学诊断目的的临床化学测定中所进行的那样。