公开(公告)号：WO2006056579A3

公开(公告)日：2006-06-01

申请号：PCT/EP2005/056178

申请日：2005-11-23

Applicant: SPINOMIX S.A. ,  RIDA, Amar

Inventor： RIDA, Amar

IPC: G01N33/543 ,  H01F1/00

Abstract: Bio-functionalized magnetic particles have a non-magnetic material matrix which supports core/shell magnetic elements composed from a ferromagnetic core material and a shell material. The shell material can be chosen either among an antiferromagnetic material, a ferromagnetic material of a kind different from the core ferromagnetic material or a metal material. By a proper choice of materials and dimension tuning of both the core and the shell as well as the amount and the concentration of the magnetic elements within the non- magnetic matrix, the bio-functionalized magnetic particles is tailored to exhibit an enhanced magnetic energy. When subjected to an alternating magnetic field, the magnetic particles exhibit specific rotational dynamics in correspondence with the amplitude and the frequency of the applied magnetic filed. Aggregation structures of the magnetic particles are controlled and manipulated by the alternating magnetic field. The magnetic particles are functionalized by specific ligands for the probing and manipulating of biomolecules and chemical substances.

公开(公告)号：WO2012035508A2

公开(公告)日：2012-03-22

申请号：PCT/IB2011/054035

申请日：2011-09-15

Applicant: SPINOMIX S.A. ,  RIDA, Amar ,  MERMOD, Nicolas ,  FRANCOIS, Patrice ,  LAZAREVIC, Vladimir ,  SCHRENZEL, Jacques

Inventor： RIDA, Amar ,  MERMOD, Nicolas ,  FRANCOIS, Patrice ,  LAZAREVIC, Vladimir ,  SCHRENZEL, Jacques

IPC: G01N33/86

CPC classification number: G01N33/86 ,  C12Q1/56 ,  G01N33/56938 ,  G01N2333/75 ,  G01N2333/974

Abstract: A method for separating target molecules or particles from a fibrinogen containing sample comprises: (a) trapping the said target molecules or particles in a fibrin network by converting at least partially the fibrinogen contained in the sample into fibrin; (b) retracting the said fibrin network to form a fibrin clot; (c) separating the said fibrin clot from the surrounding sample medium,

Abstract translation: 用于从含有纤维蛋白原的样品中分离目标分子或颗粒的方法包括：（a）通过将所述样品中所含的纤维蛋白原至少部分转变成纤维蛋白，将所述目标分子或颗粒捕获在纤维蛋白网络中 ; （b）回缩所述纤维蛋白网络以形成纤维蛋白凝块; （c）将所述纤维蛋白凝块与周围的样品介质分开，

公开(公告)号：WO2008010111A2

公开(公告)日：2008-01-24

申请号：PCT/IB2007/052410

申请日：2007-06-21

Applicant: SPINOMIX S.A. ,  RIDA, Amar

Inventor： RIDA, Amar

CPC classification number: B01F13/0809 ,  B01F13/0059 ,  B82Y5/00 ,  B82Y25/00 ,  H01F1/0054

Abstract: A device for manipulating and mixing magnetic particles (3) in a surrounding liquid medium, comprises at least one couple of magnetic poles (1,1') facing each other across a gap, the facing poles diverging from a narrow end of the gap to a large end of the gap, the poles (1,1') forming part of an electromagnetic circuit and being arranged to provide a magnetic field gradient in the gap region; and a reaction chamber (2) that is a part of a fluidic network for containing the said magnetic particles in suspension and placed in the gap of the said electromagnets poles (1,1'). The reaction chamber (2) preferably has at least one part which has a diverging cavity, arranged co-divergently in the diverging gap between the poles.

Abstract translation: 用于在周围液体介质中操纵和混合磁性颗粒（3）的装置包括至少一对磁极（1,1'），所述磁极跨越间隙彼此面对，所述相对磁极发散 从所述间隙的窄端到所述间隙的大端，所述极（1,1'）形成电磁电路的一部分并被布置为在所述间隙区域中提供磁场梯度; 和作为流体网络的一部分的反应室（2），用于容纳悬浮的所述磁性颗粒并放置在所述电磁体磁极（1,1'）的间隙中。 反应室（2）优选具有至少一个具有发散空腔的部分，该发散空腔在两极之间的发散间隙中共同地分散布置。

公开(公告)号：WO2008007270A2

公开(公告)日：2008-01-17

申请号：PCT/IB2007/052409

申请日：2007-06-21

Applicant: SPINOMIX S.A. ,  RIDA, Amar

Inventor： RIDA, Amar

CPC classification number: B01F13/0809 ,  B01F13/0059 ,  B82Y5/00 ,  B82Y25/00 ,  H01F1/0054

Abstract: A method of mixing magnetic particles (3) in a reaction chamber (2) that is part of a microfluidic device and that contains the said particles in suspension, comprises the steps: (a) providing an electromagnetic means (1,1 ',6, 7) to generate magnetic field sequences having polarity and intensity that vary in time and a magnetic field gradient that covers the whole space of the said reaction chamber (2); (b) applying a first magnetic field sequence to separate or confine the particles (3) so the particles occupy a sub-volume in the volume of the reaction chamber (2); (c) injecting a defined volume of the said reagent in the reaction chamber; and (d) applying a second magnetic field sequence to leads the particles (3) to be homogenously distributed and dynamically moving over a substantial portion of the whole reaction chamber volume.

Abstract translation: 一种在作为微流体装置的一部分的反应室（2）中混合磁性颗粒（3）并且包含处于悬浮液中的所述颗粒的方法包括以下步骤：（a）提供电磁 用于产生磁场序列的装置（1,1'，6,7），所述磁场序列具有随时间变化的极性和强度以及覆盖所述反应室（2）的整个空间的磁场梯度; （b）施加第一磁场序列以分离或限制粒子（3），使得粒子占据反应室（2）的体积中的子体积; （c）将预定体积的所述试剂注入反应室中; （d）施加第二磁场序列以引导颗粒（3）均匀分布并在整个反应室体积的大部分上动态移动。

公开(公告)号：WO2016034564A1

公开(公告)日：2016-03-10

申请号：PCT/EP2015/069907

申请日：2015-09-01

Applicant: SELEXIS S.A. ,  SPINOMIX S.A.

Inventor： MERMOD, Nicolas ,  HARRAGHY, Niamh ,  DROZ, Xuan ,  RIDA, Amar ,  GIROD, Pierre-Alain ,  REGAMEY, Alexandre ,  COLOMBET, Thierry

IPC: B01L3/00 ,  C12N15/10

CPC classification number: G01N15/1056 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/023 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0867 ,  C12N5/0682 ,  C12N2510/02 ,  G01N33/54326 ,  G01N33/54333 ,  G01N33/54366 ,  G01N33/56966 ,  G01N33/57492 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1081

Abstract: The invention discloses a method for selecting cells depending on their level of displaying and preferably secreting a protein of interest from a population of heterogeneously expressing cells, comprising: (a) contacting said cells with magnetic beads coated with an affinity group to the said cells, (b) mixing the said magnetic beads with the cells to capture the cells displaying/secreting the protein of interest, (c) performing at least one washing step to remove the non-captured cells, and (d) recovering the cells to which that magnetic beads have bound.

Abstract translation: 本发明公开了一种根据其显示水平选择细胞的方法，优选从不均匀表达的细胞群体分泌感兴趣的蛋白质，其包括：（a）使所述细胞与亲和基团包被的磁珠接触所述细胞， （b）将所述磁珠与细胞混合以捕获显示/分泌感兴趣蛋白质的细胞，（c）进行至少一个洗涤步骤以除去未捕获的细胞，和（d）回收所述细胞， 磁珠已经结合。

公开(公告)号：WO2011151804A1

公开(公告)日：2011-12-08

申请号：PCT/IB2011/052440

申请日：2011-06-03

Applicant: SPINOMIX S.A. ,  RIDA, Amar ,  VARIDEL, Thierry

Inventor： RIDA, Amar ,  VARIDEL, Thierry

IPC: B01L3/00 ,  G01N33/543

CPC classification number: B01L3/502 ,  B01L3/502715 ,  B01L3/50825 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/06 ,  B01L2300/0672 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0655 ,  G01N33/54326

Abstract: A fluidic assay system assembly comprising: (a) A disposable fluidic cartridge (1 ) comprising at least one reaction chamber (3) connected to a network of fluidic channels (2) with at least one inlet channel and one outlet channel. The said inlet and outlet channels end at the down side of the fluidic cartridge with at least two connecting pores (4), (4'); (b) A disposable vessel (5) comprising a connection tube (21 ) immersed in a sample container (6) and ended at the cap of the vessel with an external connection pore (7); (c) A fluidic manifold (8) that is interdependent with the bulk system (12) comprising a fluidic network connected (9) to active fluidic parts (10), (1 1 ). The said channel network ends at the top side of the fluidic manifold with at least one connecting pore (13). Wherein the first and the second pores of the fluidic cartridge are interfaced by direct physical contact with the sample container and the manifold pores, respectively.

Abstract translation: 一种流体测定系统组件，包括：（a）一次性流体盒（1），其包括连接到具有至少一个入口通道和一个出口通道的流体通道（2）网络的至少一个反应室（3）。 所述入口和出口通道在具有至少两个连接孔（4），（4'）的流体盒的下侧端部处停止。 （b）一个一次性容器（5），其包括浸入样品容器（6）中的一个连接管（21），并用外部连接孔（7）结束在容器的盖上。 （c）与本体系统（12）相互依赖的流体歧管（8），包括连接到主动流体部件（10），（11）的流体网络。 所述通道网络在具有至少一个连接孔（13）的流体歧管的顶侧处终止。 其中流体盒的第一和第二孔分别通过与样品容器和歧管孔直接物理接触而接合。