公开(公告)号：WO2020014190A1

公开(公告)日：2020-01-16

申请号：PCT/US2019/040945

申请日：2019-07-09

Applicant: SANDSTONE DIAGNOSTICS, INC.

Inventor： SCHAFF, Ulrich ,  HONG, Kyungjin ,  FREDRIKSEN, Laura ,  IACOVETTI, Gabriella ,  EPPERSON, Jon ,  RAHIMIAN, Ali ,  SOMMER, Greg ,  HONG, Sean ,  KENDALL, Eric

IPC: B01D21/26 ,  B04B5/04 ,  G01N33/49

Abstract: A centrifugal cartridge or disk used for extraction of light supernatant fractions from fluid samples is described, particularly for non-homogenous fluid biological samples such as whole blood. The device may be used to collect cell-free blood plasma or a fraction of whole blood containing target cells such as leukocytes. Single or multiple channels are described, including channels with passive valves, gaskets, receiving cavities, inlet holes, capillary wicking ridges, distal cavities for cell retention, separator gel, and density medium. Centrifugal action causes whole blood in a receiving cavity to pass into one or more channels where it separates into blood cells, plasma and optionally fractions of an intermediate density. After spin, the plasma returns to the receiving cavity by way of the one or more channels for extraction through the inlet hole or other inwardly located hole. Disposable cartridges are constructed of monolithic top and bottom plates, which may be joined together by an elastomeric outer seal.

公开(公告)号：WO2019240959A1

公开(公告)日：2019-12-19

申请号：PCT/US2019/034663

申请日：2019-05-30

Applicant: SANDSTONE DIAGNOSTICS, INC.

Inventor： SCHAFF, Ulrich ,  HONG, Kyungjin ,  FREDRIKSEN, Laura ,  EPPERSON, Jon

IPC: B01L1/02 ,  B01L1/04 ,  B03B13/02 ,  B03B13/04 ,  B03B13/06 ,  G01N15/06

Abstract: A device and method for automated imaging of samples are described, particularly for non-homogeneous fluid biological samples. One purpose is particle quantification and other analysis. A device comprises a fluidic disk, a monolithic optical subsystem comprising a camera, imaging optics, rotational and focus motors, controller, and user interface. In a method, the fluidic disk receives a biological sample containing particles of interest and distributes them into multiple channels of defined thickness. Spatially distinct portions of the sample are drawn into each channel. The fluidic disk is then rotated by a motor such that a portion of each channel comes into alignment with the imaging optics and then each portion is imaged sequentially, thereby compensating for sample non-homogeneity. Embodiments include monochromatic LED illumination and image processing and particle counting in the channel images.

公开(公告)号：WO2014074737A1

公开(公告)日：2014-05-15

申请号：PCT/US2013/068991

申请日：2013-11-07

Applicant: SANDSTONE DIAGNOSTICS, INC.

Inventor： SCHAFF, Ulrich ,  SOMMER, Greg ,  TOMKINS-TINCH, Christopher

IPC: C12Q1/06 ,  C12Q1/02 ,  G01N33/48

CPC classification number: B04B5/0407 ,  B04B9/10 ,  C12Q1/06 ,  G01N15/042 ,  G01N2015/0065 ,  G01N2015/045 ,  G01N2015/055

Abstract: A method and device performing the method for estimation of cell count, such as sperm cell count, is disclosed. The device may be a kit including a cartridge configured to hold fluid, such as seminal fluid, and an instrument configured to centrifuge the cartridge. The cartridge and instrument are configured such that, during operation or centrifugation, they are securely attached to each other. The cartridge has a component with a defined cross-sectional volume. The defined cross-sectional volume is used to mark the component with markings, allowing a user of the device to read the markings and estimate cell volume and, thus, concentration. Various embodiments of the device are disclosed.

Abstract translation: 公开了一种执行细胞计数估计方法（如精子细胞计数）的方法和装置。 该装置可以是包括构造成保持诸如精液体之类的流体的药筒和被配置为离心药筒的器械的药盒。 盒和仪器被配置为使得在操作或离心期间它们被牢固地附接到彼此。 盒具有具有限定的横截面体积的部件。 定义的横截面体积用于用标记来标记组件，允许设备的用户读取标记并估计细胞体积，从而评估细胞浓度。 公开了该装置的各种实施例。

公开(公告)号：WO2021211473A1

公开(公告)日：2021-10-21

申请号：PCT/US2021/026931

申请日：2021-04-12

Applicant: SANDSTONE DIAGNOSTICS, INC.

Inventor： SCHAFF, Ulrich ,  LE, Angela ,  PAN, Tifany ,  HONG, Kyungjin ,  NEAL, Clara

IPC: B05B5/12 ,  B04B5/04 ,  B04B1/00 ,  B04B5/00 ,  B01D21/26 ,  B04B1/02 ,  G01N33/48

Abstract: Embodiments of a single-tube rotor for a portable and compact centrifugal system are described. Embodiments include a monolithic rotor, suitable for manufacturing in a straight-pull injection mold, with an attachment hub, fixed retainer for exactly one sample tube, arms for the retainer, and a thin, aerodynamic counterweight. Embodiments include a counterweight and tube retainer angled downward; a central clearance volume for manual placement of a sample tube; and dimensions optimized to just fill a fixed rotational circle. Embodiments include a centrifuge with an enclosure, hinged lid, motor, and timer, free of both user controls and user displays.

公开(公告)号：WO2019215273A1

公开(公告)日：2019-11-14

申请号：PCT/EP2019/061902

申请日：2019-05-09

Applicant: F. HOFFMANN-LA ROCHE AG ,  ROCHE DIAGNOSTICS GMBH ,  ROCHE MOLECULAR SYSTEMS, INC. ,  SANDSTONE DIAGNOSTICS, INC.

Inventor： EPPERSON, Jon ,  FREDRIKSEN, Laura ,  HONG, Kyungjin ,  LI, Jenq-Thun ,  PARIKH, Bhairavi, Rajiv ,  SCHAFF, Ulrich

IPC: G01N33/543 ,  G01N33/569

Abstract: A cartridge for determining a concentration of target cells within a sample includes a separation portion and a detection portion. The separation portion includes a first and second surface defining a separation chamber. The separation portion can contain a density medium having a density greater than a density of a first portion of the sample and less than a density of a second portion of the sample (that includes the target cells). The separation chamber can be fluidically coupled to an inlet reservoir such that the sample can pass from the inlet reservoir to the separation chamber during rotation. The detection portion includes a detection surface that forms a boundary of a detection chamber. The detection surface is nonparallel to the first surface such that the target cells impinge on the detection surface when passing into the detection chamber. The detection surface is configured to capture the target cells.

公开(公告)号：WO2018217184A1

公开(公告)日：2018-11-29

申请号：PCT/US2017/033710

申请日：2017-05-20

Applicant: SANDSTONE DIAGNOSTICS, INC.

Inventor： ULRICH, Schaff ,  JON, Epperson ,  LAURA, Fredriksen ,  KIRK, Harmon

IPC: A61B10/00 ,  B01L3/00 ,  G01F19/00

Abstract: A cup used to receive, hold, measure and pour liquids, such a specimens, such as for a medical assay, is described. The cup comprises an open receiving upper chamber, two open sample-directing channels, and an open, calibrated and marked measuring column. When sitting on a level surface, fluid flows from the upper chamber through the sample-directing channels into the measuring column. A foot is under each fluid-directing channel. The base of the measuring column functions as a third foot. Cups nest, with the two feet of the upper cup sitting into two sample-directing columns of lower cup. The measuring column and upper chamber taper to permit nesting. If the cup is tipped, it rests on two of three of: the two feet and the base of the measuring column, and a point on a lower perimeter of the upper chamber, such that fluid will not spill.

公开(公告)号：WO2014124179A1

公开(公告)日：2014-08-14

申请号：PCT/US2014/015170

申请日：2014-02-06

Applicant: SANDSTONE DIAGNOSTICS, INC.

Inventor： SCHAFF, Ulrich ,  TOMKINS-TINCH, Christopher ,  SAUERS, Jason ,  SOMMER, Greg

IPC: G01N1/38 ,  G01N35/00

CPC classification number: B01L3/502 ,  B01F11/0002 ,  B01F11/0068 ,  B01F13/0052 ,  B01F13/0064 ,  B01F15/00844 ,  B01F15/0233 ,  B01F2215/0454 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0681 ,  B01L2300/0858 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0409 ,  B04B5/0407 ,  G01N1/4077 ,  G01N33/491 ,  G01N2001/4083

Abstract: The disclosure describes methods and devices with which to process and analyze difficult chemical, biological, environmental samples including but not limited to those containing bulk solids or particulates. The disclosure includes a cartridge which contains a separation tube as well as one or more valves and cavities for receiving raw sample materials and for directing and containing various fluids or samples. The cartridge may contain a separation fluid or density medium of defined density, and structures which direct particulates toward defined regions of the cartridge. Embodiments can include a rotational device for rotating the cartridge at defined rotational rates for defined time intervals. Embodiments allowing multiple assays from a single sample are also disclosed. In some embodiments, this device is used for direct processing and chemical analysis of food, soil, blood, stool, motor oil, semen, and other samples of interest.

Abstract translation: 本公开描述了用于处理和分析困难的化学，生物，环境样品的方法和装置，包括但不限于含有散装固体或微粒的那些。 本公开包括一个盒，其包含分离管以及一个或多个用于接收原始样品材料并用于引导和容纳各种流体或样品的阀和空腔。 墨盒可以包含具有确定密度的分离流体或密度介质，以及将颗粒引导到墨盒的限定区域的结构。 实施例可以包括用于以限定的时间间隔以限定的旋转速度旋转盒的旋转装置。 还公开了允许来自单个样品的多个测定的实施方案。 在一些实施方案中，该装置用于食品，土壤，血液，粪便，机油，精液和其他感兴趣的样品的直接加工和化学分析。