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    METHODS FOR NUCLEIC ACID FINGERPRINT ANALYSIS
    9.
    发明申请
    METHODS FOR NUCLEIC ACID FINGERPRINT ANALYSIS 审中-公开

    公开(公告)号：WO2002064835A3

    公开(公告)日：2002-08-22

    申请号：PCT/US2002/003168

    申请日：2002-01-31

    Applicant: AMBION, INC. ,  BROWN, David ,  WINKLER, Matthew, M. ,  LAWRENCE, Frank ,  SHELTON, Jeffrey

    Inventor： BROWN, David ,  WINKLER, Matthew, M. ,  LAWRENCE, Frank ,  SHELTON, Jeffrey

    IPC: C12Q1/68

    Abstract: Methods are provided for generating nucleic acid fingerprints from complex nucleic acid populations. The methods rely on the addition of a nucleic acid tag with at least two functional domains to members of the complex population being assessed. Primers specific to the appended tag and arbitrary or adapter-specific primers are used to amplify subsets of the complex population. The amplified population is then used to generate labeled products for comparative expression analysis using the functional domain of the appended tag sequence that was not used for amplification. The separation of amplification and labeling substantially reduces the number of false positives common in amplification fingerprinting experiments by removing all amplification products from the analysis that do not derive from the anchored ends of the nucleic acid population.

    METHODS FOR NUCLEIC ACID FINGERPRINT ANALYSIS
    10.
    发明申请
    METHODS FOR NUCLEIC ACID FINGERPRINT ANALYSIS 审中-公开
    核酸指纹分析方法

    公开(公告)号：WO2002064835A2

    公开(公告)日：2002-08-22

    申请号：PCT/US2002/003168

    申请日：2002-01-31

    Applicant: AMBION, INC. ,  BROWN, David ,  WINKLER, Matthew, M. ,  LAWRENCE, Frank ,  SHELTON, Jeffrey

    Inventor： BROWN, David ,  WINKLER, Matthew, M. ,  LAWRENCE, Frank ,  SHELTON, Jeffrey

    IPC: C12Q1/68

    CPC classification number: C12Q1/6809 ,  C12Q2539/113 ,  C12Q2525/191 ,  C12Q2525/179

    Abstract: Methods are provided for generating nucleic acid fingerprints from complex nucleic acid populations. The methods rely on the addition of a nucleic acid tag with at least two functional domains to members of the complex population being assessed. Primers specific to the appended tag and arbitrary or adapter-specific primers are used to amplify subsets of the complex population. The amplified population is then used to generate labeled products for comparative expression analysis using the functional domain of the appended tag sequence that was not used for amplification. The separation of amplification and labeling substantially reduces the number of false positives common in amplification fingerprinting experiments by removing all amplification products from the analysis that do not derive from the anchored ends of the nucleic acid population.

    Abstract translation: 提供了从复杂核酸群体产生核酸指纹的方法。 所述方法依赖于将正在评估的复合群体的成员添加具有至少两个功能结构域的核酸标签。 用于附加标签​​和任意或接头特异性引物特异性的引物用于扩增复合群体的子集。 然后使用扩增的群体产生用于比较表达分析的标记产物，其使用未用于扩增的附加标签序列的功能域。 扩增和标记的分离大大减少了扩增指纹图谱实验中常见的假阳性数目，其中从分析中除去不是从核酸群体的锚定末端得到的所有扩增产物。

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