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    ENZYMATIC DIGESTION OF TISSUE
    2.
    发明申请
    ENZYMATIC DIGESTION OF TISSUE 审中-公开
    组织消毒

    公开(公告)号：WO2006089259A1

    公开(公告)日：2006-08-24

    申请号：PCT/US2006/005903

    申请日：2006-02-17

    Applicant: AMBION, INC. ,  LATHAM, Gary ,  PASLOSKE, Brittan, L. ,  PELTIER, Heidi

    Inventor： LATHAM, Gary ,  PASLOSKE, Brittan, L. ,  PELTIER, Heidi

    IPC: C12Q1/68 ,  C12N15/10

    CPC classification number: C12Q1/6806 ,  C12N15/1003

    Abstract: The present invention concerns a compositions and method for isolating a nucleic acid from a cell-containing sample. There is disclosed a method comprising obtaining at least one cell-containing sample, which comprises a cell containing nucleic acid, obtaining at least one catabolic enzyme, obtaining at least one nuclease inhibitor, preparing an admixture of the sample, the catabolic enzyme, and the nuclease inhibitor, maintaining the admixture under conditions where the catabolic enzyme is active, and agitating the admixture, where the sample is digested to produce a nucleic acid-containing lysate of the sample.

    Abstract translation: 本发明涉及从含细胞的样品中分离核酸的组合物和方法。 公开了一种方法，包括获得至少一种含细胞的样品，其包含含有核酸的细胞，获得至少一种分解代谢酶，获得至少一种核酸酶抑制剂，制备样品，分解代谢酶和 核酸酶抑制剂，在分解代谢酶活性的条件下保持混合物，并搅拌混合物，其中样品被消化以产生样品的含核酸裂解物。

    METHODS AND COMPOSITIONS CONCERNING siRNA'S AS MEDIATORS OF RNA INTERFERENCE
    3.
    发明申请
    METHODS AND COMPOSITIONS CONCERNING siRNA'S AS MEDIATORS OF RNA INTERFERENCE 审中-公开
    关于siRNA作为RNA干扰介质的方法和组合物

    公开(公告)号：WO2006078414A2

    公开(公告)日：2006-07-27

    申请号：PCT/US2005/046779

    申请日：2005-12-21

    Applicant: AMBION, INC. ,  FORD, Lance, P. ,  KREBS, Joseph

    Inventor： FORD, Lance, P. ,  KREBS, Joseph

    IPC: C12N15/11 ,  C12N15/113

    CPC classification number: C12N15/111 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2330/30 ,  C12Y102/01012 ,  C12Y502/01008

    Abstract: The present invention concerns an isolated siRNA of from about 5 to about 20 nucleotides that mediates RNA interference. Also disclosed are methods of reducing expression of a target gene in a cell comprising obtaining at least one siRNA of 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 basepairs in length; and delivering the siRNA into the cell. The siRNAs can be chemically synthesized RNA or an analog of a naturally occurring RNA.

    Abstract translation: 本发明涉及介导RNA干扰的约5至约20个核苷酸的分离的siRNA。 还公开了减少细胞中靶基因表达的方法，包括获得至少一种5,6,7,8,9,10,11,12,13,14,15,16,17,18,19种的siRNA ，或20个碱基对长度; 并将siRNA递送到细胞中。 siRNA可以是化学合成的RNA或天然存在的RNA的类似物。

    METHODS AND COMPOSITIONS FOR TAILING AND AMPLIFYING RNA
    5.
    发明申请
    METHODS AND COMPOSITIONS FOR TAILING AND AMPLIFYING RNA 审中-公开
    用于尾部和扩增RNA的方法和组合物

    公开(公告)号：WO2006029350A2

    公开(公告)日：2006-03-16

    申请号：PCT/US2005/032227

    申请日：2005-09-07

    Applicant: AMBION, INC. ,  MURPHY, George, L. ,  WHITLEY, Penn, J.

    Inventor： MURPHY, George, L. ,  WHITLEY, Penn, J.

    CPC classification number: C12P19/34 ,  C12Q1/6806 ,  C12Q2525/204 ,  C12Q2525/173 ,  C12Q2527/137 ,  C12Q2521/325

    Abstract: The present invention provides methods and compositions that enable tagging and amplification of targeted RNA molecules. A targeted RNA molecule is any non-polyadenylated RNA molecule including, for example, miRNA, siRNA, rRNA, tRNA, synthetic RNA, or non-polyadenylated mRNA such as mRNA from bacteria. In certain aspects, the invention provides methods and compositions for the genome-wide expression analysis of bacterial genes. Significantly, the methods enable genome-wide expression analysis in circumstances where bacterial numbers were previously too low to purify adequate amounts of RNA for DNA microarray analysis or other applications. Such methods are particularly useful for the study of bacterial gene expression during host-cell infection. The invention also provides kits for tagging and amplifying targeted RNA molecules.

    Abstract translation: 本发明提供了能够标记和扩增靶向RNA分子的方法和组合物。 靶向RNA分子是任何非聚腺苷酸化RNA分子，包括例如miRNA，siRNA，rRNA，tRNA，合成RNA或非聚腺苷酸化mRNA，例如来自细菌的mRNA。 在某些方面，本发明提供了用于细菌基因的全基因组表达分析的方法和组合物。 值得注意的是，这些方法能够在细菌数量过低而无法纯化足够量的RNA用于DNA微阵列分析或其他应用的情况下进行全基因组表达分析。 这些方法对于研究宿主细胞感染期间的细菌基因表达特别有用。 本发明还提供用于标记和扩增靶向RNA分子的试剂盒。

    METHODS AND COMPOSITIONS FOR PREPARING CAPPED RNA
    6.
    发明申请
    METHODS AND COMPOSITIONS FOR PREPARING CAPPED RNA 审中-公开
    制备CAPPED RNA的方法和组合物

    公开(公告)号：WO2006004648A1

    公开(公告)日：2006-01-12

    申请号：PCT/US2005/022710

    申请日：2005-06-28

    Applicant: AMBION, INC. ,  LABOURIER, Emmanuel ,  PASLOSKE, Brittan, L.

    Inventor： LABOURIER, Emmanuel ,  PASLOSKE, Brittan, L.

    IPC: C12P19/34

    CPC classification number: C12N15/79

    Abstract: The present invention concerns methods and compositions for increasing the yield of capped and full-length RNA transcripts produced in in vitro transcription reactions. Such methods and compositions can be used for cost-efficient, large-scale production of capped full-­length RNA transcripts that can be subsequently translated. Methods and compositions involve reaction conditions that promote such production and include the implementation of fed-batch introduction of GTP, which competes with a cap analog.

    Abstract translation: 本发明涉及用于增加在中产生的封端和全长RNA转录物的产量的方法和组合物

    METHODS AND COMPOSITIONS RELATING TO LABELED RNA MOLECULES THAT REDUCE GENE EXPRESSION
    9.
    发明申请
    METHODS AND COMPOSITIONS RELATING TO LABELED RNA MOLECULES THAT REDUCE GENE EXPRESSION 审中-公开
    与减少基因表达的标记RNA分子相关的方法和组合物

    公开(公告)号：WO2003106631A2

    公开(公告)日：2003-12-24

    申请号：PCT/US2003/018627

    申请日：2003-06-12

    Applicant: AMBION, INC. ,  FORD, Lance, P. ,  BYROM, Mike ,  PASLOSKE, Brittan, L.

    Inventor： FORD, Lance, P. ,  BYROM, Mike ,  PASLOSKE, Brittan, L.

    IPC: C12N

    CPC classification number: C12N15/1135 ,  C12N9/22 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1136 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2330/30 ,  C12Y301/26003

    Abstract: The present invention concerns methods and compositions involving labeled, doublestranded RNA (dsRNA), including siRNA, capable of triggering RNA-mediated interference (RNAi) in a cell. Compositions of the invention include labeled dsRNA for RNAi, which may be a single strand of RNA that basepairs with itself or two separate RNA strands. In some embodiments, the label is fluorescent. The present invention further concerns methods for preparing such composition and kits for implementing such methods. Other methods of the invention include ways of using labeled dsRNA for RNAi.

    Abstract translation: 本发明涉及涉及标记的双链RNA（dsRNA）的方法和组合物，其包括能够引发细胞中RNA介导的干扰（RNAi）的siRNA。 本发明的组合物包括用于RNAi的标记的dsRNA，其可以是具有其自身或两个单独的RNA链的碱基对的单链RNA。 在一些实施方案中，标记是荧光的。 本发明还涉及制备这种组合物的方法和用于实施这些方法的试剂盒。 本发明的其它方法包括使用标记的dsRNA用于RNAi的方法。

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