公开(公告)号：WO2012019159A1

公开(公告)日：2012-02-09

申请号：PCT/US2011/046849

申请日：2011-08-05

申请人： DANISCO US INC. ,  BERGSMA, Martien, H. ,  CHOTANI, Gopal, K. ,  CUEVAS, William, A. ,  DUAN, Gang ,  LEE, Sung, Ho ,  QIAN, Ying ,  SHARMA, Vivek ,  SHETTY, Jayarama, K. ,  STROHM, Bruce, A. ,  TEUNISSEN, Pauline Johanna, Maria ,  XU, Hongxian

发明人： BERGSMA, Martien, H. ,  CHOTANI, Gopal, K. ,  CUEVAS, William, A. ,  DUAN, Gang ,  LEE, Sung, Ho ,  QIAN, Ying ,  SHARMA, Vivek ,  SHETTY, Jayarama, K. ,  STROHM, Bruce, A. ,  TEUNISSEN, Pauline Johanna, Maria ,  XU, Hongxian

IPC分类号： C12P7/06

CPC分类号： C12P7/06 ,  C12P7/04 ,  C12P7/14 ,  C12P7/16 ,  C12P7/18 ,  C12P7/46 ,  C12P7/56 ,  C12P13/04 ,  C12P13/14 ,  C12P19/20 ,  Y02E50/10 ,  Y02E50/17

摘要： Embodiments of the present disclosure relate to a process for producing downstream products, such as fermentable sugars and end products, from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.

摘要翻译： 本公开的实施方案涉及通过糖化和/或发酵从淀粉底物生产下游产物如可发酵糖和终产物的方法。 糖化在pH为5.0至8.0的范围内由葡糖淀粉酶有效地催化。 在6.0或更高的pH下，葡糖淀粉酶相对于其最大活性具有至少50％的活性。 糖化和发酵可以作为同时糖化和发酵（SSF）方法进行。

公开(公告)号：WO2009052101A1

公开(公告)日：2009-04-23

申请号：PCT/US2008/079827

申请日：2008-10-14

申请人： DANISCO US, INC. ,  LANTERO, Oreste, J. ,  SHETTY, Jayarama, K. ,  BRENEMAN, Suzanne

发明人： LANTERO, Oreste, J. ,  SHETTY, Jayarama, K. ,  BRENEMAN, Suzanne

IPC分类号： C12N9/30 ,  C12N9/34 ,  C12N9/62 ,  C12Q1/40 ,  C12N9/58

CPC分类号： C12N9/2428 ,  C12N9/242 ,  C12N9/58 ,  C12P7/10 ,  C12P19/02 ,  Y02E50/16

摘要： The present invention relates to an enzyme blend composition comprising a glucoamylase, an acid stable alpha amylase, and an acid fungal protease. The present invention is further directed to a method for producing end products such as alcohols from fermentable sugars, comprising the steps of: (a) contacting a slurry comprising a milled grain that contains starch with an alpha amylase to produce a liquefact; (b) contacting the liquefact with a glucoamylase, an acid stable alpha amylase, and an acid fungal protease, to produce fermentable sugars; and (c) fermenting the fermentable sugars in the presence of a fermenting organism to produce end products.

摘要翻译： 本发明涉及包含葡糖淀粉酶，酸稳定的α淀粉酶和酸性真菌蛋白酶的酶混合组合物。 本发明还涉及从可发酵糖生产最终产品如醇的方法，包括以下步骤：（a）使含有淀粉的研磨颗粒与α-淀粉酶的浆料接触以产生液化; （b）使液化酶与葡糖淀粉酶，酸稳定的α淀粉酶和酸性真菌蛋白酶接触以产生可发酵的糖; 和（c）在发酵生物存在下发酵可发酵糖以产生最终产物。

公开(公告)号：WO2008118382A3

公开(公告)日：2009-02-26

申请号：PCT/US2008003788

申请日：2008-03-21

申请人： DANISCO US INC GENENCOR DIV ,  MCDONALD HUGH C ,  POULOSE AYROOKARAN J ,  SHETTY JAYARAMA K

发明人： MCDONALD HUGH C ,  POULOSE AYROOKARAN J ,  SHETTY JAYARAMA K

IPC分类号： C11D3/22 ,  C11D3/386 ,  C12N9/10

CPC分类号： C11D3/38636 ,  C11D3/222 ,  C12N9/107 ,  D06L1/14 ,  D06L4/40

摘要： The present invention provides compositions comprising a transglucosidase enzyme and a natural gum polysaccharide, wherein the natural gum polysaccharide is a substrate for the transglucosidase enzyme. The present invention also provides methods for using a transglucosidase enzyme to degrade natural gum polysaccharide. In some preferred embodiments, the compositions and methods find use in cleaning applications.

摘要翻译： 本发明提供了包含转葡糖苷酶和天然胶多糖的组合物，其中天然胶多糖是转葡糖苷酶的底物。 本发明还提供了使用转葡糖苷酶降解天然树胶多糖的方法。 在一些优选的实施方案中，组合物和方法可用于清洁应用。

公开(公告)号：WO2006073843A3

公开(公告)日：2007-04-05

申请号：PCT/US2005046474

申请日：2005-12-21

申请人： GENENCOR INT ,  DUAN GANG ,  DUNN-COLEMAN NIGEL ,  LANTERO ORESTE J JR ,  PILGRIM CRAIG E ,  SHETTY JAYARAMA K

发明人： DUAN GANG ,  DUNN-COLEMAN NIGEL ,  LANTERO ORESTE J JR ,  PILGRIM CRAIG E ,  SHETTY JAYARAMA K

IPC分类号： C12P7/06

CPC分类号： C12N9/58 ,  A23L13/424 ,  A23L33/18 ,  C12N9/48 ,  C12P7/06 ,  C12P7/10 ,  C12P19/02 ,  Y02E50/16 ,  Y02E50/17

摘要： The invention is directed to methods of producing ethanol and decreasing residual starch production in a no cook fermentation comprising contacting granular starch containing substrates with a granular starch hydrolyzing enzyme, a protease, and a fermenting microorganism under suitable fermentation conditions at a temperature below the starch gelatinization temperature of the starch substrate to produce ethanol, wherein the ethanol production is increased and the amount of residual starch is decreased compared to a substantially similar method conducted without the protease.

摘要翻译： 本发明涉及生产乙醇的方法，并且在无烹饪发酵中减少残余淀粉的产生，包括在合适的发酵条件下，在低于淀粉糊化温度的温度下，使含有颗粒淀粉的底物与颗粒淀粉水解酶，蛋白酶和发酵微生物接触 淀粉底物的温度以产生乙醇，其中与基本相似的无蛋白酶进行的方法相比，乙醇生产增加，残留淀粉的量降低。

公开(公告)号：WO2005118800A1

公开(公告)日：2005-12-15

申请号：PCT/US2004/040040

申请日：2004-11-30

申请人： GENENCOR INTERNATIONAL, INC. ,  DUNN-COLEMAN, Nigel ,  LANTERO, JR., Oreste ,  SHETTY, Jayarama, K. ,  LANTZ, Suzanne, E. ,  PEPSIN, Michael, J.

发明人： DUNN-COLEMAN, Nigel ,  LANTERO, JR., Oreste ,  SHETTY, Jayarama, K. ,  LANTZ, Suzanne, E. ,  PEPSIN, Michael, J.

IPC分类号： C12N9/30

CPC分类号： C12P7/06 ,  C12N9/242 ,  C12N9/2428 ,  C12P19/14 ,  C12Y302/01001 ,  Y02E50/17

摘要： The present invention relates to a granular starch hydrolyzing enzyme composition comprising an acid stable alpha amylase (asAA) having granular starch hydrolyzing activity. The invention also relates to a one-step method for producing an alcohol which comprises contacting a granular starch substrate with an acid-stable alpha amylase (asAA) having granular starch hydrolyzing (GSH) activity and a glucoamylase (GA) in a fermentation step which also comprises ethanologenic microorganisms at a temperature of 25 - 40°C to obtain a fermentation broth having 5 to 20% ethanol.

摘要翻译： 本发明涉及一种含有具有淀粉淀粉水解活性的酸稳定性α淀粉酶（asAA）的颗粒淀粉水解酶组合物。 本发明还涉及一种生产醇的一步法，该方法包括在发酵步骤中使颗粒淀粉基质与具有颗粒状淀粉水解（GSH）活性的酸稳定性α淀粉酶（asAA）和葡糖淀粉酶（GA）接触， 还在25-40℃的温度下还含有产乙醇微生物，得到具有5〜20％乙醇的发酵液。

公开(公告)号：WO2005117756A3

公开(公告)日：2005-12-15

申请号：PCT/US2005/018214

申请日：2005-05-24

申请人： GENENCOR INTERNATIONAL, INC. ,  DUNN-COLEMAN, Nigel ,  FISKE, Susan, M. ,  LANTZ, Suzanne, E. ,  NEEFE-KRUITHOF, Paulien ,  PEPSIN, Michael, J. ,  SHETTY, Jayarama, K.

发明人： DUNN-COLEMAN, Nigel ,  FISKE, Susan, M. ,  LANTZ, Suzanne, E. ,  NEEFE-KRUITHOF, Paulien ,  PEPSIN, Michael, J. ,  SHETTY, Jayarama, K.

IPC分类号： C12N9/30 ,  C12P7/06 ,  C12N9/34 ,  C12R1/885

摘要： The present invention relates to an acid-stable alpha amylase (asAA) derived from a strain of Aspergillus kawachi, which has granular starch hydrolyzing (GSH) activity, the heterologous expression of the asAA having GSH activity in filamentous fungal host cells and enzyme compositions including the same which optionally include glucoamylase.

公开(公告)号：WO2005052148A3

公开(公告)日：2005-09-15

申请号：PCT/US2004038713

申请日：2004-11-18

申请人： GENENCOR INT ,  BALDWIN TOBY L ,  BOWER BENJAMIN S ,  CHOTANI GOPAL K ,  DUNN-COLEMAN NIGEL ,  LANTERO JR ORESTE ,  LANTZ SUZANNE E ,  PEPSIN MICHAEL J ,  SHETTY JAYARAMA K ,  STROHM BRUCE A ,  WANG HUAMING

发明人： BALDWIN TOBY L ,  BOWER BENJAMIN S ,  CHOTANI GOPAL K ,  DUNN-COLEMAN NIGEL ,  LANTERO JR ORESTE ,  LANTZ SUZANNE E ,  PEPSIN MICHAEL J ,  SHETTY JAYARAMA K ,  STROHM BRUCE A ,  WANG HUAMING

IPC分类号： C07H21/04 ,  C12N1/14 ,  C12N1/16 ,  C12N9/30 ,  C12N9/32 ,  C12N9/34 ,  C12N15/74 ,  C12P19/14 ,  C12P19/20 ,  C12P19/24 ,  C08B30/00 ,  C12N9/62 ,  C12P19/00

CPC分类号： C12P19/14 ,  C12N1/14 ,  C12N9/242 ,  C12N9/2428 ,  C12P19/02 ,  C12P19/04 ,  C12P19/20 ,  C12P19/24 ,  Y02E50/17 ,  Y02P20/52

摘要： The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.

摘要翻译： 本发明涉及用于生产具有葡糖淀粉酶活性的异源颗粒淀粉水解酶（GSHE）的丝状真菌宿主细胞，特别是木霉属宿主细胞。 本发明还涉及生产葡萄糖浆的方法，其包括在等于或低于颗粒淀粉的凝胶化温度的温度下将由颗粒淀粉基质获得的颗粒淀粉浆与α-淀粉酶和GSHE同时接触以获得组合物 的葡萄糖浆。

公开(公告)号：WO2015021600A1

公开(公告)日：2015-02-19

申请号：PCT/CN2013/081383

申请日：2013-08-13

申请人： DANISCO US INC. ,  ADAMS, Christian D. ,  HUA, Ling ,  SHETTY, Jayarama K. ,  SONG, Danfeng ,  TANG, Zhongmei ,  XIE, Zhiyong ,  ZHANG, Bo ,  ZOU, Zhengzheng

发明人： ADAMS, Christian D. ,  HUA, Ling ,  SHETTY, Jayarama K. ,  SONG, Danfeng ,  TANG, Zhongmei ,  XIE, Zhiyong ,  ZHANG, Bo ,  ZOU, Zhengzheng

IPC分类号： C07K14/195 ,  C12N9/26 ,  C12N15/56

CPC分类号： C12N9/2425 ,  C12Y302/01002

摘要： The present teachings provide a beta amylase with maltogenic properties. Non-naturally occuring nucleic acids encoding the beta amylase and variants thereof, expression vectors, formulations, and host cells are also provided. Additional embodiments of the present teachings provide various methods of use and methods of manufacturing.

摘要翻译： 本发明提供具有麦芽糖特性的β淀粉酶。 还提供了编码β淀粉酶及其变体，表达载体，制剂和宿主细胞的非天然存在的核酸。 本教导的另外的实施例提供了各种使用方法和制造方法。

公开(公告)号：WO2013022799A1

公开(公告)日：2013-02-14

申请号：PCT/US2012/049646

申请日：2012-08-03

申请人： DANISCO US INC. ,  BERGSMA, Martien, H. ,  CALABRIA, Anthony, R. ,  CHOTANI, Gopal, K. ,  CUEVAS, William, A. ,  DUAN, Gang ,  LEE, Sung Ho ,  QIAN, Ying ,  SHARMA, Vivek ,  SHETTY, Jayarama, K. ,  STROHM, Bruce, A. ,  TEUNISSEN, Pauline, Johanna Maria ,  XU, Hongxian

发明人： BERGSMA, Martien, H. ,  CALABRIA, Anthony, R. ,  CHOTANI, Gopal, K. ,  CUEVAS, William, A. ,  DUAN, Gang ,  LEE, Sung Ho ,  QIAN, Ying ,  SHARMA, Vivek ,  SHETTY, Jayarama, K. ,  STROHM, Bruce, A. ,  TEUNISSEN, Pauline, Johanna Maria ,  XU, Hongxian

IPC分类号： C12P5/00 ,  C12N9/24

CPC分类号： C12P5/007 ,  C12N9/1085 ,  C12N9/2428 ,  C12Y205/0101 ,  C12Y302/01003

摘要： Embodiments of the present disclosure relate to a process for producing isoprenoid precursor molecules and/or isoprenoids from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.

摘要翻译： 本公开的实施方案涉及通过糖化和/或发酵从淀粉底物生产类异戊二烯前体分子和/或类异戊二烯的方法。 糖化在pH为5.0至8.0的范围内由葡糖淀粉酶有效地催化。 在6.0或更高的pH下，葡糖淀粉酶相对于其最大活性具有至少50％的活性。 糖化和发酵可以作为同时糖化和发酵（SSF）方法进行。

公开(公告)号：WO2012149288A1

公开(公告)日：2012-11-01

申请号：PCT/US2012/035413

申请日：2012-04-27

申请人： DANISCO US INC. ,  LEE, Sung Ho ,  SHETTY, Jayarama K. ,  TEUNISSEN, Paula Johanna Maria

发明人： LEE, Sung Ho ,  SHETTY, Jayarama K. ,  TEUNISSEN, Paula Johanna Maria

IPC分类号： C12P19/20 ,  C12N9/24

CPC分类号： C12P19/20 ,  C12N9/2428 ,  Y02E50/16

摘要： Embodiments of the present disclosure relate to a process for producing downstream products, such as high-density glucose syrups and high-glucose fermentation feedstock, from a starch-containing substrate without a pH adjustment before the saccharification step. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.2 to 5.6.

摘要翻译： 本公开的实施方案涉及在糖化步骤之前从没有pH调节的含淀粉的底物生产下游产物如高密度葡萄糖浆和高葡萄糖发酵原料的方法。 糖化在pH5.2至5.6的范围内由葡糖淀粉酶有效地催化。