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    GLUCOAMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME
    4.
    发明申请
    GLUCOAMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
    葡糖淀粉酶变体和编码多核苷酸的多核苷酸

    公开(公告)号：WO2017066255A1

    公开(公告)日：2017-04-20

    申请号：PCT/US2016/056549

    申请日：2016-10-12

    Applicant: NOVOZYMES A/S ,  NELSON, Beth

    Inventor： NELSON, Beth ,  SVENDSEN, Allan ,  SRIVASTAVA, Vivek ,  MALTEN, Marco ,  POULSEN, Thomas A. ,  RAMAIYA, Preethi ,  GUPTA, Shikha ,  RAO, Vasudeva P. ,  BALUMURI, Padmavathi

    IPC: C12P19/02 ,  C12P19/20 ,  C12N9/34

    CPC classification number: C12N9/2428 ,  C12P19/02 ,  C12P19/14 ,  C12P19/20 ,  C12Y302/01003

    Abstract: The present invention relates to glucoamylase variants having an increase in specific activity and/or an increase in melting temperature measured by TSA of at least 0.5 degrees Celsius, and further wherein the variants have at least at least 85%, at least 90%, at least 95% identity, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

    Abstract translation: 本发明涉及葡糖淀粉酶变体，其通过TSA测量的比活性增加和/或熔解温度增加至少0.5摄氏度，并且另外其中变体具有至少85 与SEQ ID NO：1的氨基酸序列具有至少90％，至少90％，至少95％同一性，至少96％，至少97％，至少98％，或至少99％，但小于100％ 编号：3。本发明还涉及编码变体的多核苷酸; 包含所述多核苷酸的核酸构建体，载体和宿主细胞; 以及使用这些变体的方法。

    CELLULOSE AND/OR HEMICELLULOSES DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF
    10.
    发明申请
    CELLULOSE AND/OR HEMICELLULOSES DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF 审中-公开
    纤维素和/或致细胞毒素降解大蒜素的酶及其用途

    公开(公告)号：WO2014028774A3

    公开(公告)日：2014-05-08

    申请号：PCT/US2013055200

    申请日：2013-08-15

    Applicant: BANGLADESH JUTE RES INST

    Inventor： ISLAM MOHAMMED SHAHIDUL ,  HOSSEN MOHAMMED MOSADDEQUE ,  HAQUE MOHAMMED SAMIUL ,  ALAM MOHAMMED MONJURUL ,  ALAM MAQSUDUL

    IPC: C12N9/42 ,  C12N15/00

    CPC classification number: C12N9/2402 ,  C12N9/2408 ,  C12N9/2428 ,  C12N9/2437 ,  C12N9/244 ,  C12N9/2445 ,  C12N9/2471 ,  C12N9/2482 ,  C12N9/2494 ,  C12N9/88 ,  C12Y302/01037 ,  C12Y302/01051 ,  C12Y302/01055 ,  C12Y302/01059 ,  C12Y302/01075 ,  C12Y302/01089 ,  C12Y302/0113 ,  C12Y302/01177

    Abstract: The present invention provides nucleotide sequences of Macrophomina phaseolina ("M. phaseolina") that encodes proteins/enzymes with cellulolytic activity, including a cellulase activity, a endoglucanase, a cellobiohydrolase, a &bgr;-glucosidase, a a-glucosidase, a xylanase, a mannanse, a &bgr;-xylosidase, a a-xylosidase, a galactosidase, an arabinofuranosidase, a a- fucosidases, a &bgr;-galactanase, an unsaturated &bgr;-glucuronyl hydrolase and/or oligomerase activity. Vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes and methods for modifying the enzymes in order to improve their desirable characteristics. The enzymes of the invention can be used in a variety of, but not limited to, pharmaceutical, agricultural, food and feed processing, biofuel, energy efficiency and industrial contexts. These enzymes are also useful for complete hydrolysis of lignocellulosic biomass into simple sugar that can then be fermented to liquid fuels and chemical feedstocks.

    Abstract translation: 本发明提供了编码具有纤维素分解活性的蛋白质/酶的Macrophomina phaseolina（“M. phaseolina”）的核苷酸序列，包括纤维素酶活性，内切葡聚糖酶，纤维二糖水解酶，葡糖苷酶，α-葡糖苷酶，木聚糖酶， 甘露聚糖，α-木糖苷酶，α-木糖苷酶，半乳糖苷酶，阿拉伯呋喃糖苷酶，α-岩藻糖苷酶，β-半乳糖苷酶，不饱和的 - 葡萄糖醛酸水解酶和/或寡聚酶活性。 也提供了包含和/或由该酶基因的核苷酸序列组成的载体，表达构建体和宿主细胞。 本发明还提供了用于生产酶的方法和用于改变酶的方法，以便改善其期望的特性。 本发明的酶可以多种但不限于制药，农业，食品和饲料加工，生物燃料，能源效率和工业环境。 这些酶也可用于将木质纤维素生物质完全水解成单糖，然后将其发酵成液体燃料和化学原料。

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