公开(公告)号：WO2014152281A2

公开(公告)日：2014-09-25

申请号：PCT/US2014/027158

申请日：2014-03-14

Applicant: THE BROAD INSTITUTE, INC. ,  THE GENERAL HOSPITAL CORP (dba MASSACHUSETTS GENERAL HOSPITAL)

Inventor： BHATTACHARYYA, Roby ,  HUNG, Deb ,  LIVNY, Jonathan

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/112 ,  C12Q2600/158

Abstract: The present disclosure relates to method of distinguishing between two or more species of one or more organisms in a sample, by contacting a biological sample comprising ribosomal ribonucleic acid (rRNA) with a set of antisense probes, wherein the set of probes contains at least one detectable probe that is specific for a target rRNA sequence of each species to be tested, and wherein the individual probes specific for each species comprises less than about 85% sequence identity; and, detecting hybridization between one or more of the probes and the rRNA, thereby distinguishing between two or more species in a sample.

Abstract translation: 本公开涉及通过将包含核糖核糖核酸（rRNA）的生物样品与一组反义探针接触来区分样品中两种或更多种生物体的方法，其中该组探针含有至少一种 可检测的探针，其对于待测试的每种物质的靶rRNA序列是特异性的，并且其中每种物种特异性的各个探针包含小于约85％的序列同一性; 并且检测一个或多个探针和rRNA之间的杂交，从而区分样品中的两种或更多种。

公开(公告)号：WO2014205296A1

公开(公告)日：2014-12-24

申请号：PCT/US2014/043295

申请日：2014-06-19

Applicant: THE BROAD INSTITUTE, INC. ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  THE GENERAL HOSPITAL CORP (DBA MASSACHUSETTS GENERAL HOSPITAL) ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： BERNSTEIN, Bradley ,  GOREN, Alon ,  NUSBAUM, Chad ,  RAM, Oren ,  ROTEM, Assaf ,  TARJAN, Daniel ,  XING, Jeffrey ,  REGEV, Aviv

IPC: C12N15/10

CPC classification number: C12Q1/6874 ,  C12N15/1082 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N33/541 ,  C12Q2521/301 ,  C12Q2522/101 ,  C12Q2523/101 ,  C12Q2535/122 ,  C12Q2563/179

Abstract: Disclosed are methods for shearing and tagging chromatin DNA. The disclosed methods include contacting chromatin DNA with at least one transposome, that includes a transposase enzyme. The transposon is made up of a first DNA molecule that includes a first transposase recognition site and a second DNA molecule that includes a second transposase recognition site, wherein the transposase integrates the first and second DNA molecules into chromatin DNA. The first and second DNA molecules of the transposon can be disconnected, such that upon integration of the transposon the chromatin bound DNA is sheared and tagged with the first and second DNA molecules, for example to prepare a library of sheared and tagged chromatin DNA fragments.

Abstract translation: 公开了剪切和标记染色质DNA的方法。 所公开的方法包括使染色质DNA与至少一个转座子接触，包括转座酶。 转座子由包含第一转座酶识别位点的第一DNA分子和包含第二转座酶识别位点的第二DNA分子组成，其中转座酶将第一和第二DNA分子整合到染色质DNA中。 转座子的第一个和第二个DNA分子可以断开，使得在整合转座子后，将染色质结合的DNA剪切并用第一和第二个DNA分子进行标记，例如制备剪切和标记的染色质DNA片段的文库。

公开(公告)号：WO2018005417A1

公开(公告)日：2018-01-04

申请号：PCT/US2017/039389

申请日：2017-06-27

Applicant: THE GENERAL HOSPITAL CORP DBA MASSACHUSETTS GENERAL HOSPITAL

Inventor： WU, Meixiong

IPC: A61K31/05 ,  A61K31/155 ,  A61K31/192 ,  A61K31/216 ,  A61K31/352 ,  A61K31/429 ,  A61K31/4439 ,  A61K31/498 ,  A61K31/7056 ,  A61K45/00 ,  A61P7/04

Abstract: A method of stimulating platelet formation using a drug that stimulates mitochondrial biogenesis is described. The drug can be used to treat a subject that has been diagnosed with thrombocytopenia or a relatively low platelet count, or it can be used to stimulate platelet generation in vitro or ex vivo . Low-level light (LLL) therapy can be used together with the drug to stimulate mitochondrial biogenesis.

Abstract translation: 描述了使用刺激线粒体生物发生的药物刺激血小板形成的方法。 该药物可用于治疗已被诊断患有血小板减少症或相对较低的血小板计数的受试者，或其可用于体外或体外刺激血小板产生， 。 低浓度光线（LLL）疗法可与药物一起用于刺激线粒体生物发生。