公开(公告)号：WO02031503A1

公开(公告)日：2002-04-18

申请号：PCT/SE2001/002185

申请日：2001-10-08

IPC: G01N33/543 ,  G01N33/541 ,  G01N33/566 ,  G01N33/58

CPC classification number: G01N33/541 ,  G01N33/566

Abstract: The invention relates to a method of determining an analyte in a sample, especially a high concentration analyte, comprises the steps of: a) contacting the sample with a specified amount of a receptor which binds specifically to the analyte to form an analyte/receptor complex, said specified amount of receptor being in excess of that required to bind all analyte in the sample, b) isolating on a solid phase a specified fraction of the amount of receptor contacted with the analyte, including analyte/receptor complex and unreacted receptor, c) detecting the amount of analyte/receptor complex in said isolated specified fraction, and d) from the detected amount analyte/receptor complex, determining the concentration of analyte in the sample. The invention also relates to test kits for carrying out the method.

Abstract translation: 本发明涉及一种测定样品，特别是高浓度分析物中的分析物的方法，包括以下步骤：a）使样品与指定量的特异性结合分析物结合以形成分析物/受体复合物的受体接触 所述指定量的受体超过结合样品中所有分析物所需的受体，b）在固相上分离与分析物接触的受体的量的特定部分，包括分析物/受体复合物和未反应的受体，c ）检测所述分离的特定级分中的分析物/受体复合物的量，以及d）从检测到的量的分析物/受体复合物中测定样品中分析物的浓度。 本发明还涉及用于实施该方法的测试试剂盒。

公开(公告)号：WO1995033204A1

公开(公告)日：1995-12-07

申请号：PCT/EP1995001829

申请日：1995-05-15

Applicant: BAYER AKTIENGESELLSCHAFT ,  STÖCKER, Ronald, Helmut ,  DOTH, Margit

Inventor： BAYER AKTIENGESELLSCHAFT

IPC: G01N33/541

CPC classification number: G01N33/541 ,  G01N33/538 ,  G01N33/54306

Abstract: The present invention relates to an immunoassay and a method for the determination of antigens, in which an antigen is bound to a specific antibody, the conjugate formed is reacted with an immobilized capture antibody which recognizes and binds the non-bound antibody and the quantity of free or bound antibody is determined in the supernatant or on the matrix.

Abstract translation: 本发明涉及免疫测定法和测定抗原的方法，其中抗原与特异性抗体结合，所形成的缀合物与识别和结合非结合抗体的固定化捕获抗体反应， 在上清液或基质上测定游离或结合的抗体。

公开(公告)号：WO2016113719A1

公开(公告)日：2016-07-21

申请号：PCT/IB2016/050229

申请日：2016-01-18

Applicant: DIASORIN S.P.A. ,  UNIVERSITE CATHOLIQUE DE LOUVAIN

Inventor： GRUSON, Damien ,  ROUSSEAU, Michel ,  ZIEROLD, Claudia ,  BONELLI, Fabrizio ,  BLOCKI, Frank

IPC: G01N33/541 ,  G01N33/543 ,  G01N33/74 ,  G01N33/82

CPC classification number: G01N33/74 ,  G01N33/541 ,  G01N33/543 ,  G01N33/82 ,  G01N2800/347

Abstract: The present invention relates to a method for predicting or stratifying the risk of morbidity and/or mortality in a chronic heart failure patient. Levels of 1,25-dihydroxyvitamin D (1,25(OH) 2 D) are measured in a biological sample and taken together with parathyroid hormone (PTH) levels to provide a ratio indicative of risk of chronic heart failure morbidity and/or mortality.

Abstract translation: 本发明涉及用于预测或分层慢性心力衰竭患者的发病率和/或死亡风险的方法。 在生物样品中测量1,25-二羟维生素D（1,25（OH）2D）的水平，并与甲状旁腺激素（PTH）水平一起提供指示慢性心力衰竭发病率和/或死亡率风险的比例。

公开(公告)号：WO2007032778A2

公开(公告)日：2007-03-22

申请号：PCT/US2006/010809

申请日：2006-03-23

Applicant: XENCOR, INC. ,  MUCHHAL, Umesh, S. ,  DESJARLAIS, John, R. ,  MOORE, Gregory, L.

Inventor： MUCHHAL, Umesh, S. ,  DESJARLAIS, John, R. ,  MOORE, Gregory, L.

IPC: G01N33/541 ,  G01N33/68

CPC classification number: G01N33/56977 ,  B01J2219/00725 ,  G01N33/541 ,  G01N33/6845 ,  G01N2333/70539

Abstract: Methods, apparatuses, and compounds for screening or detecting binding of candidate peptides to an MHC protein is provided. A first component including at least one candidate peptides and a second component including at least one MHC protein are contacted. One of the components is immobilized on a solid support. The presence, absence, or quantity of binding of the peptide and said MHC protein is then determined.

Abstract translation: 提供了用于筛选或检测候选肽与MHC蛋白的结合的方法，装置和化合物。 接触包含至少一种候选肽的第一组分和包含至少一种MHC蛋白的第二组分。 其中一个组分固定在固体支持物上。 然后确定肽和所述MHC蛋白的结合的存在，不存在或数量。

公开(公告)号：WO2005094381A3

公开(公告)日：2006-07-13

申请号：PCT/US2005010932

申请日：2005-03-31

Applicant: RULES BASED MEDICINE INC ,  SPAIN MICHAEL D ,  CHANDLER MARK B

Inventor： SPAIN MICHAEL D ,  CHANDLER MARK B

IPC: G01N33/543 ,  C12Q1/68 ,  G01N21/62 ,  G01N33/53 ,  G01N33/536 ,  G01N33/541 ,  G01N33/566 ,  G01N35/08 ,  G06F19/00

CPC classification number: G01N33/536 ,  C12Q1/6816 ,  G01N33/541 ,  G01N33/54333 ,  G16H50/50 ,  G16H50/70 ,  Y02A90/26 ,  C12Q2563/137

Abstract: A method for the multiplexed diagnosis of a plurality of different biomolecules in a fluid sample substantially simultaneously is provided. In accordance with a method of the invention, a substantial fraction of biomolecules in a fluid sample are complexed with a universal label and a secondary labeling reagent. Flow cytometric measurements may be used to identify and quantify, in real-time, by detecting the secondary reagent and universal label present in any of said complexes. The inventive technology enables the simultaneous, and automated, detection and interpretation of multiple biomolecules while also reducing the cost of performing diagnostic and genetic assays.

Abstract translation: 提供了基本上同时多次诊断流体样本中的多个不同生物分子的方法。 根据本发明的方法，流体样品中的大部分生物分子与通用标记物和次级标记试剂复合。 流式细胞术测量可用于通过检测存在于任何所述复合物中的次级试剂和通用标记来实时鉴定和定量。 本发明的技术能够同时和自动化地检测和解释多种生物分子，同时也降低了进行诊断和遗传测定的成本。

公开(公告)号：WO2002083262A1

公开(公告)日：2002-10-24

申请号：PCT/US2002/007079

申请日：2002-03-07

Applicant: BIOERGONOMICS, INC. ,  COLLINS, Daniel, P. ,  SHAUT, David, M. ,  HAPKE, Joel, H.

Inventor： COLLINS, Daniel, P. ,  SHAUT, David, M. ,  HAPKE, Joel, H.

IPC: B01D21/26

CPC classification number: G01N33/537 ,  C07K16/2896 ,  C12N5/0087 ,  G01N33/5094 ,  G01N33/541 ,  G01N33/566 ,  G01N33/56966 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/25375

Abstract: The invention provides compositions and methods for cell separation. These reagents and techniques specifically agglutinate cells via surface antigen recognition and can be used to recover even rare cell types in high yield.

Abstract translation: 本发明提供了用于细胞分离的组合物和方法。 这些试剂和技术通过表面抗原识别特异性地凝集细胞，并可用于以高产率回收甚至罕见的细胞类型。

公开(公告)号：WO2016091755A1

公开(公告)日：2016-06-16

申请号：PCT/EP2015/078693

申请日：2015-12-04

Applicant: F. HOFFMANN-LA ROCHE AG ,  ROCHE DIAGNOSTICS GMBH ,  ROCHE DIAGNOSTICS OPERATIONS, INC.

Inventor： FAATZ, Elke ,  GERG, Michael ,  JOSEL, Hans-Peter ,  VOGL, Christian

IPC: G01N33/537 ,  G01N33/541

CPC classification number: G01N33/537 ,  C07K16/26 ,  G01N33/541 ,  G01N33/82

Abstract: The present invention concerns an in vitro method for measurement of 25-hydroxyvitamin D, wherein the potentially interfering compound 24,25-dihydroxyvitamin D 3 is blocked by a binding agent specifically binding to 24,25-dihydroxyvitamin D 3 and not binding to 25-hydroxyvitamin D.

Abstract translation: 本发明涉及用于测量25-羟基维生素D的体外方法，其中潜在的干扰化合物24,25-二羟基维生素D3被特异性结合24,25-二羟基维生素D 3而不与25-羟基维生素D结合的结合剂阻断 。

公开(公告)号：WO2014205296A1

公开(公告)日：2014-12-24

申请号：PCT/US2014/043295

申请日：2014-06-19

Applicant: THE BROAD INSTITUTE, INC. ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  THE GENERAL HOSPITAL CORP (DBA MASSACHUSETTS GENERAL HOSPITAL) ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： BERNSTEIN, Bradley ,  GOREN, Alon ,  NUSBAUM, Chad ,  RAM, Oren ,  ROTEM, Assaf ,  TARJAN, Daniel ,  XING, Jeffrey ,  REGEV, Aviv

IPC: C12N15/10

CPC classification number: C12Q1/6874 ,  C12N15/1082 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N33/541 ,  C12Q2521/301 ,  C12Q2522/101 ,  C12Q2523/101 ,  C12Q2535/122 ,  C12Q2563/179

Abstract: Disclosed are methods for shearing and tagging chromatin DNA. The disclosed methods include contacting chromatin DNA with at least one transposome, that includes a transposase enzyme. The transposon is made up of a first DNA molecule that includes a first transposase recognition site and a second DNA molecule that includes a second transposase recognition site, wherein the transposase integrates the first and second DNA molecules into chromatin DNA. The first and second DNA molecules of the transposon can be disconnected, such that upon integration of the transposon the chromatin bound DNA is sheared and tagged with the first and second DNA molecules, for example to prepare a library of sheared and tagged chromatin DNA fragments.

Abstract translation: 公开了剪切和标记染色质DNA的方法。 所公开的方法包括使染色质DNA与至少一个转座子接触，包括转座酶。 转座子由包含第一转座酶识别位点的第一DNA分子和包含第二转座酶识别位点的第二DNA分子组成，其中转座酶将第一和第二DNA分子整合到染色质DNA中。 转座子的第一个和第二个DNA分子可以断开，使得在整合转座子后，将染色质结合的DNA剪切并用第一和第二个DNA分子进行标记，例如制备剪切和标记的染色质DNA片段的文库。

公开(公告)号：WO2013070167A1

公开(公告)日：2013-05-16

申请号：PCT/SE2012/051224

申请日：2012-11-09

Applicant: VISA, Neus ,  ÖSTLUND FARRANTAS, Ann-Kristin ,  BÖHM, Stefanie Gabriele ,  EBERLE, Andrea

Inventor： VISA, Neus ,  ÖSTLUND FARRANTAS, Ann-Kristin ,  BÖHM, Stefanie Gabriele ,  EBERLE, Andrea

IPC: C12N15/10 ,  G01N33/536

CPC classification number: C12Q1/6806 ,  C12Q1/6876 ,  G01N33/541 ,  G01N33/6875

Abstract: The present invention provides an external standard in the form of a nucleic acid-antibody complex to be used in a chromatin immunoprecipitation method.

Abstract translation: 本发明提供了用于染色质免疫沉淀方法的核酸 - 抗体复合物形式的外部标准。

公开(公告)号：WO2009010976A3

公开(公告)日：2010-02-25

申请号：PCT/IL2008000988

申请日：2008-07-16

Applicant: AFFISINK BIOTECHNOLOGY LTD ,  PATCHORNIK GUY

Inventor： PATCHORNIK GUY

IPC: C07K14/76 ,  C07K1/00

CPC classification number: C07K16/00 ,  C12N7/00 ,  C12N2710/00051 ,  G01N33/541

Abstract: A composition-of-matter comprising at least one ligand capable of binding a target molecule or cell of interest is provided, The composition comprising the at least one ligand being a composite ligand which comprises a scaffold moiety attached to at least one target recognition moiety capable of directly or indirectly binding the target molecule, cell or virus, the at least one ligand being attached to at least two coordinating moieties selected capable of directing the composition-of-matter to form a non-covalent complex when co-incubated with a coordinator ion or molecule.

Abstract translation: 提供了包含至少一种能够结合目标分子或目的细胞的配体的物质组合物。该组合物包含至少一种配体，其是复合配体，其包含连接至至少一个目标识别部分的支架部分 直接或间接地结合靶分子，细胞或病毒，所述至少一个配体连接至至少两个选择的配位部分，所述配位部分能够与协调器共培养时引导物质组合物形成非共价复合物 离子或分子。