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    COMPARATIVE ANALYSIS OF NUCLEIC ACIDS USING POPULATION TAGGING
    3.
    发明申请
    COMPARATIVE ANALYSIS OF NUCLEIC ACIDS USING POPULATION TAGGING 审中-公开
    使用人口标签对核酸进行比较分析

    公开(公告)号：WO2002061143A2

    公开(公告)日：2002-08-08

    申请号：PCT/US2002/003097

    申请日：2002-01-31

    Applicant: AMBION, INC. ,  WINKLER, Matthew, M. ,  BROWN, David

    Inventor： WINKLER, Matthew, M. ,  BROWN, David

    IPC: C12Q1/68

    CPC classification number: C12Q1/6809 ,  C12Q2531/143 ,  C12Q2525/161 ,  C12Q2525/155

    Abstract: Disclosed are methods that allow one or more nucleic acid targets to be compared across two or more nucleic acid samples. Nucleic acid tags are appended to the samples to be assessed, such that each sample has a unique tag. The tagged nucleic acids are then mixed, and the targets within the mixture are amplified. The amplification products are distinguished using the unique tag domains to reveal the abundance of the amplification products derived from each sample, which correlates to the relative abundance of the target in the samples.

    Abstract translation: 公开了允许在两个或多个核酸样品之间比较一个或多个核酸靶标的方法。 将核酸标签附加到待评估的样品上，使得每个样品具有唯一的标记。 然后将标记的核酸混合，并扩增混合物内的靶。 使用独特的标签结构区分扩增产物，以揭示衍生自每个样品的扩增产物的丰度，其与样品中靶标的相对丰度相关。

    COMPETITIVE POPULATION NORMALIZATION FOR COMPARATIVE ANALYSIS OF NUCLEIC ACID SAMPLES
    4.
    发明申请
    COMPETITIVE POPULATION NORMALIZATION FOR COMPARATIVE ANALYSIS OF NUCLEIC ACID SAMPLES 审中-公开
    核酸样品比较分析的竞争性人口普查

    公开(公告)号：WO02061140A9

    公开(公告)日：2003-11-27

    申请号：PCT/US0202892

    申请日：2002-01-31

    Applicant: AMBION INC ,  BROWN DAVID ,  WINKLER MATTHEW M

    Inventor： BROWN DAVID ,  WINKLER MATTHEW M

    IPC: C12Q1/68

    CPC classification number: C12Q1/6809 ,  C12Q2527/143 ,  C12Q2525/161 ,  C12Q2525/143 ,  C12Q2525/155

    Abstract: Methods are disclosed that convert two or more complex nucleic acid samples into a single collection of normalized target molecules that can be used to compare the abundance of each of the targets in the original samples. Multiple RNA or DNA samples are uniquely tagged and pooled to create a sample mixture. A defined set of target within the sample mixture is converted to approximately equal amounts of nucleic acid by one of several methods employing primer extension with a set of target specific primers. The concentration of target specific primers is equal and limiting for all targets, therefore an appropriate number of primer extension cycles converts all targets to similar final concentrations of product nucleic acid. The different tags appended to the sample nucleic acids from each sample population unique. These different tags are used to generate RNA or DNA , molecules for analysis that derive form each of the input samples. The disclosed methods are primarily intended to enhance gene array analysis, however, any method used to compare multiple nucleic acid targets form different samples will benefit form the invention.

    Abstract translation: 公开了将两个或更多个复合核酸样品转化成归一化的靶分子的单一集合的方法，其可用于比较原始样品中每个靶的丰度。 多个RNA或DNA样品被独特地标记和合并以产生样品混合物。 样品混合物中定义的一组靶标通过使用一组靶特异性引物的引物延伸的几种方法之一转化为大致相等量的核酸。 靶标特异性引物的浓度对于所有靶标是相同的并且是有限的，因此适当数量的引物延伸周期将所有靶标转化为产物核酸的相似终浓度。 来自每个样本群体的示例核酸附加的不同标签是唯一的。 这些不同的标签用于产生RNA或DNA，用于分析的分子，其从每个输入样品得到。 所公开的方法主要用于增强基因阵列分析，然而，用于比较多个核酸靶标形成不同样品的任何方法将从本发明中受益。

    COMPETITIVE AMPLIFICATION OF FRACTIONATED TARGETS FROM MULTIPLE NUCLEIC ACID SAMPLES
    5.
    发明申请
    COMPETITIVE AMPLIFICATION OF FRACTIONATED TARGETS FROM MULTIPLE NUCLEIC ACID SAMPLES 审中-公开
    来自多种核酸样品的分离目标的竞争性放大

    公开(公告)号：WO02061145A3

    公开(公告)日：2003-07-24

    申请号：PCT/US0203169

    申请日：2002-01-31

    Applicant: AMBION INC ,  WINKLER MATTHEW M ,  BROWN DAVID

    Inventor： WINKLER MATTHEW M ,  BROWN DAVID

    IPC: C12Q1/68

    CPC classification number: C12Q1/6851 ,  C12Q2545/107

    Abstract: Disclosed are methods that allow one or more targets to be compared across two or more nucleic acid populations. The methods rely on first mixing sample populations that are being compared. The sample mixture is then divided into target fractions using hybridization to polynucleotides or oligonucleotides that can be separated form the sample mixture. the target fraction(s) are independently amplified such that the targets from each sample compete for amplification reagents. The amplification products are quantified in a manner that differentiates the sample from which a particular amplification product arose. The relative abundance of amplification products descended from each sample population reflects the level of target present in each of the original samples, providing a direct comparison of the abundance of the target sequences in the samples being characterized.

    Abstract translation: 公开了允许在两个或多个核酸群体上比较一个或多个靶标的方法。 该方法依赖于第一次混合正在比较的样本群体。 然后将样品混合物与可与样品混合物分离的多核苷酸或寡核苷酸杂交分成目标级分。 靶分离物被独立地扩增，使得来自每个样品的靶标竞争扩增试剂。 扩增产物以区分特定扩增产物产生的样品的方式进行定量。 来自每个样本群体的扩增产物的相对丰度反映了每个原始样品中存在的靶标水平，提供了正在表征的样品中靶序列丰度的直接比较。

    COMPETITIVE AMPLIFICATION OF FRACTIONATED TARGETS FROM MULTIPLE NUCLEIC ACID SAMPLES
    6.
    发明申请
    COMPETITIVE AMPLIFICATION OF FRACTIONATED TARGETS FROM MULTIPLE NUCLEIC ACID SAMPLES 审中-公开
    来自多种核酸样品的分离目标的竞争性放大

    公开(公告)号：WO2002061145A2

    公开(公告)日：2002-08-08

    申请号：PCT/US2002/003169

    申请日：2002-01-31

    Applicant: AMBION, INC. ,  WINKLER, Matthew, M. ,  BROWN, David

    Inventor： WINKLER, Matthew, M. ,  BROWN, David

    IPC: C12Q1/68

    CPC classification number: C12Q1/6851 ,  C12Q2545/107

    Abstract: Disclosed are methods that allow one or more targets to be compared across two or more nucleic acid populations. The methods rely on first mixing sample populations that are being compared. The sample mixture is then divided into target fractions using hybridization to polynucleotides or oligonucleotides that can be separated form the sample mixture. the target fraction(s) are independently amplified such that the targets from each sample compete for amplification reagents. The amplification products are quantified in a manner that differentiates the sample from which a particular amplification product arose. The relative abundance of amplification products descended from each sample population reflects the level of target present in each of the original samples, providing a direct comparison of the abundance of the target sequences in the samples being characterized.

    Abstract translation: 公开了允许在两个或多个核酸群体上比较一个或多个靶标的方法。 该方法依赖于第一次混合正在比较的样本群体。 然后将样品混合物与可与样品混合物分离的多核苷酸或寡核苷酸杂交分成目标级分。 靶分离物被独立地扩增，使得来自每个样品的靶标竞争扩增试剂。 扩增产物以区分特定扩增产物产生的样品的方式进行定量。 从每个样本群体下降的扩增产物的相对丰度反映了每个原始样品中存在的靶标水平，提供了正在表征的样品中靶序列丰度的直接比较。

    COMPETITIVE POPULATION NORMALIZATION FOR COMPARATIVE ANALYSIS OF NUCLEIC ACID SAMPLES
    7.
    发明申请
    COMPETITIVE POPULATION NORMALIZATION FOR COMPARATIVE ANALYSIS OF NUCLEIC ACID SAMPLES 审中-公开
    核酸样品比较分析的竞争性人口普查

    公开(公告)号：WO2002061140A2

    公开(公告)日：2002-08-08

    申请号：PCT/US2002/002892

    申请日：2002-01-31

    Applicant: AMBION, INC. ,  BROWN, David ,  WINKLER, Matthew, M.

    Inventor： BROWN, David ,  WINKLER, Matthew, M.

    IPC: C12Q1/68

    CPC classification number: C12Q1/6809 ,  C12Q2527/143 ,  C12Q2525/161 ,  C12Q2525/143 ,  C12Q2525/155

    Abstract: Methods are disclosed that convert two or more complex nucleic acid samples into a single collection of normalized target molecules that can be used to compare the abundance of each of the targets in the original samples. Multiple RNA or DNA samples are uniquely tagged and pooled to create a sample mixture. A defined set of target within the sample mixture is converted to approximately equal amounts of nucleic acid by one of several methods employing primer extension with a set of target specific primers. The concentration of target specific primers is equal and limiting for all targets, therefore an appropriate number of primer extension cycles converts all targets to similar final concentrations of product nucleic acid. The different tags appended to the sample nucleic acids from each sample population unique. These different tags are used to generate RNA or DNA , molecules for analysis that derive form each of the input samples. The disclosed methods are primarily intended to enhance gene array analysis, however, any method used to compare multiple nucleic acid targets form different samples will benefit form the invention.

    Abstract translation: 公开了将两个或更多个复合核酸样品转化成归一化的靶分子的单一集合的方法，其可用于比较原始样品中每个靶的丰度。 多个RNA或DNA样品被独特地标记和合并以产生样品混合物。 样品混合物中定义的一组靶标通过使用一组靶特异性引物的引物延伸的几种方法之一转化为大致相等量的核酸。 靶标特异性引物的浓度对于所有靶标是相同的并且是有限的，因此适当数量的引物延伸周期将所有靶标转化为产物核酸的相似终浓度。 来自每个样本群体的示例核酸附加的不同标签是唯一的。 这些不同的标签用于产生RNA或DNA，用于分析的分子，其从每个输入样品得到。 所公开的方法主要用于增强基因阵列分析，然而，用于比较多个核酸靶标形成不同样品的任何方法将从本发明中受益。

    COMPARATIVE ANALYSIS OF NUCLEIC ACIDS USING POPULATION TAGGING
    9.
    发明申请
    COMPARATIVE ANALYSIS OF NUCLEIC ACIDS USING POPULATION TAGGING 审中-公开
    使用人口标签对核酸进行比较分析

    公开(公告)号：WO02061143A3

    公开(公告)日：2003-03-27

    申请号：PCT/US0203097

    申请日：2002-01-31

    Applicant: AMBION INC ,  WINKLER MATTHEW M ,  BROWN DAVID

    Inventor： WINKLER MATTHEW M ,  BROWN DAVID

    IPC: C12Q1/68

    CPC classification number: C12Q1/6809 ,  C12Q2531/143 ,  C12Q2525/161 ,  C12Q2525/155

    Abstract: Disclosed are methods that allow one or more nucleic acid targets to be compared across two or more nucleic acid samples. Nucleic acid tags are appended to the samples to be assessed, such that each sample has a unique tag. The tagged nucleic acids are then mixed, and the targets within the mixture are amplified. The amplification products are distinguished using the unique tag domains to reveal the abundance of the amplification products derived from each sample, which correlates to the relative abundance of the target in the samples.

    Abstract translation: 公开了允许在两个或多个核酸样品之间比较一个或多个核酸靶标的方法。 将核酸标签附加到待评估的样品上，使得每个样品具有唯一的标记。 然后将标记的核酸混合，并扩增混合物内的靶。 使用独特的标签结构区分扩增产物，以揭示衍生自每个样品的扩增产物的丰度，其与样品中靶标的相对丰度相关。

    METHODS FOR NUCLEIC ACID FINGERPRINT ANALYSIS
    10.
    发明申请
    METHODS FOR NUCLEIC ACID FINGERPRINT ANALYSIS 审中-公开

    公开(公告)号：WO2002064835A3

    公开(公告)日：2002-08-22

    申请号：PCT/US2002/003168

    申请日：2002-01-31

    Applicant: AMBION, INC. ,  BROWN, David ,  WINKLER, Matthew, M. ,  LAWRENCE, Frank ,  SHELTON, Jeffrey

    Inventor： BROWN, David ,  WINKLER, Matthew, M. ,  LAWRENCE, Frank ,  SHELTON, Jeffrey

    IPC: C12Q1/68

    Abstract: Methods are provided for generating nucleic acid fingerprints from complex nucleic acid populations. The methods rely on the addition of a nucleic acid tag with at least two functional domains to members of the complex population being assessed. Primers specific to the appended tag and arbitrary or adapter-specific primers are used to amplify subsets of the complex population. The amplified population is then used to generate labeled products for comparative expression analysis using the functional domain of the appended tag sequence that was not used for amplification. The separation of amplification and labeling substantially reduces the number of false positives common in amplification fingerprinting experiments by removing all amplification products from the analysis that do not derive from the anchored ends of the nucleic acid population.

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