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    DETECTING DEMENTIA AND ALZHEIMER'S DISEASE ASSOCIATED BIOMARKERS STABILIZED ON SOLID SUPPORT MATERIALS
    1.
    发明申请
    DETECTING DEMENTIA AND ALZHEIMER'S DISEASE ASSOCIATED BIOMARKERS STABILIZED ON SOLID SUPPORT MATERIALS 审中-公开
    检测DEMENTIA和ALZHEIMER的疾病相关的生物标志物稳定在固体支持材料

    公开(公告)号：WO2016081100A1

    公开(公告)日：2016-05-26

    申请号：PCT/US2015/055554

    申请日：2015-10-14

    Applicant: GE HEALTHCARE UK LIMITED ,  GENERAL ELECTRIC COMPANY

    Inventor： TATNELL, Peter James ,  HORTON, Jeffrey Kenneth

    IPC: G01N33/68

    CPC classification number: G01N33/6896 ,  C12Q1/44 ,  C12Q1/6883 ,  C12Q2600/118 ,  C12Q2600/158 ,  G01N33/6869 ,  G01N2333/55 ,  G01N2333/922 ,  G01N2800/2814 ,  G01N2800/2821 ,  G01N2800/50 ,  G01N2800/52

    Abstract: Embodiments of the invention provides a method for detecting one or more biomarkers derived from a body fluid, comprising performing one or more assays for the biomarkers from a sample of the body fluid, whereby the sample is previously preserved on a solid support; wherein a change in the biomarkers provides an indication of a biological event in the brain. The invention also provides related methods for identifying a person as being at risk of dementia or Alzheimer's disease, monitoring a person for the onset or progression of dementia or Alzheimer's disease, evaluating the effectiveness of a potential pharmaceutical agent.

    Abstract translation: 本发明的实施方案提供了用于检测源自体液的一种或多种生物标志物的方法，包括从体液样品中进行生物标志物的一种或多种测定，由此将样品预先保留在固体支持物上; 其中生物标志物的变化提供了脑中生物学事件的指示。 本发明还提供了用于鉴定患有痴呆或阿尔茨海默病风险的人的相关方法，监测患者痴呆或阿尔茨海默病的发作或进展，评估潜在药剂的有效性。

    THERMOLABILE EXONUCLEASES
    2.
    发明申请
    THERMOLABILE EXONUCLEASES 审中-公开
    可燃烧剂

    公开(公告)号：WO2016026574A1

    公开(公告)日：2016-02-25

    申请号：PCT/EP2015/001703

    申请日：2015-08-19

    Applicant: ARCTICZYMES AS ,  UNIVERSITETET I TROMSØ - NORGES ARKTISKE UNIVERSITET

    Inventor： SOLSTAD, Terese ,  ANDREASSEN, Elisabeth, Lill ,  LORENTZEN, Marit, Sjo ,  LANES, Olav ,  ELDE, Morten ,  LARSEN, Atle, Noralf ,  PIOTROWSKI, Yvonne ,  WILLASSEN, Nils, Peder

    IPC: C12N9/22

    CPC classification number: C12Q1/6848 ,  C12N9/22 ,  C12Q1/34 ,  C12Q2521/325 ,  C12Y301/11001 ,  G01N1/30 ,  G01N2333/922

    Abstract: The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID No. 1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55 °C for 10 minutes in a buffer consisting of 10 mM Tris-HCI, pH 8.5 at 25 °C, 50 mM KCI and 5 mM MgCI 2 ; (ii) is substantially specific for single stranded DNA; and (iii) has a 3'-5' exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used. The invention still further provides nucleic acids encoding said exonuclease or an enzymatically active fragment thereof and kits or compositions comprising the same.

    Abstract translation: 本发明提供了外切核酸酶或其酶促活性片段，所述外切核酸酶具有SEQ ID No.1的氨基酸序列或与其至少约50％相同的氨基酸序列，其中所述外切核酸酶或酶活性片段（i ）通过在25℃，50mM KCl和5mM MgCl 2的10mM Tris-HCl，pH8.5组成的缓冲液中在约55℃的温度下加热10分钟而基本上不可逆地灭活。 （ii）对单链DNA基本上是特异性的; 和（iii）具有3'-5'核酸外切酶活性。 本发明还提供从样品中除去单链DNA的方法，核酸扩增方法，逆转录方法以及使用其核酸外切酶或酶促活性片段的核酸序列分析方法。 本发明还提供了编码所述外切核酸酶或其酶促活性片段的核酸和包含其的试剂盒或组合物。

    USE OF RNA REMOVAL TO INITIATE PROTEIN AGGREGATION
    3.
    发明申请
    USE OF RNA REMOVAL TO INITIATE PROTEIN AGGREGATION 审中-公开
    使用RNA去除启动蛋白质聚集

    公开(公告)号：WO2014114937A1

    公开(公告)日：2014-07-31

    申请号：PCT/GB2014/050177

    申请日：2014-01-23

    Applicant: QUEEN MARY UNIVERSITY OF LONDON

    Inventor： AARUM, Johan ,  SHEER, Denise

    IPC: C12Q1/34

    CPC classification number: G01N33/6803 ,  C12Q1/34 ,  C12Q1/6869 ,  C12Q1/6883 ,  G01N33/6896 ,  G01N2333/922 ,  G01N2500/04

    Abstract: The present invention provides the use of RNA removal to initiate protein aggregation of a plurality of proteins in a cell or cell lysate. This may be used to create an in vitro model of a disease, such as a neurodegenerative disease. The present invention also provides a method for determining the efficacy of a potential anti-protein aggregation agent comprising the following steps: i) using RNA removal to initiate the aggregation of a protein in a cell or cell lysate, ii) treating the cell or cell lysate with the potential anti-protein aggregation agent before, after or during RNA removal; and iii) comparing protein aggregation in equivalent samples with and without step ii) treatment in which a decrease in protein aggregation associated with step ii) treatment indicates that the potential anti-protein aggregation agent is effective in preventing and/or reversing protein aggregation.

    Abstract translation: 本发明提供RNA去除在细胞或细胞裂解物中起始蛋白质聚集多种蛋白质的用途。 这可以用于创建疾病的体外模型，例如神经变性疾病。 本发明还提供了一种用于确定潜在的抗蛋白质聚集剂的功效的方法，其包括以下步骤：i）使用RNA去除来引发细胞或细胞裂解物中蛋白质的聚集，ii）治疗细胞或细胞 在去除RNA之前，之后或之中，潜在的抗蛋白聚集剂裂解物; 和iii）比较具有和不具有步骤的等同样品中的蛋白质聚集。ii）其中与步骤ii）处理相关的蛋白质聚集减少的处理表明潜在的抗蛋白质聚集剂有效预防和/或逆转蛋白质聚集。

    DETECTION AND REMOVAL OF MISFOLDED PROTEINS/PEPTIDES
    4.
    发明申请
    DETECTION AND REMOVAL OF MISFOLDED PROTEINS/PEPTIDES 审中-公开
    检测和清除MISS折叠的蛋白/多肽

    公开(公告)号：WO2012107567A3

    公开(公告)日：2012-11-15

    申请号：PCT/EP2012052330

    申请日：2012-02-10

    Applicant: OXPROTECT GMBH ,  KEHREL BEATE ,  BRODDE MARTIN

    Inventor： KEHREL BEATE ,  BRODDE MARTIN

    IPC: G01N33/53 ,  A61K38/00

    CPC classification number: C12N9/6435 ,  A61K38/00 ,  C12N9/6489 ,  C12Y304/21007 ,  G01N33/573 ,  G01N33/6845 ,  G01N33/6854 ,  G01N33/6893 ,  G01N33/6896 ,  G01N33/92 ,  G01N2333/47 ,  G01N2333/4709 ,  G01N2333/775 ,  G01N2333/922 ,  G01N2800/2814 ,  G01N2800/7047

    Abstract: The invention relates to the field of detecting and measuring misfolded proteins/peptides. In particular, the invention relates to the detection and measurement of misfolded proteins/peptides in bodily fluids and on cell surfaces and tissues of persons and mammals, to the detection of misfolded proteins/peptides in test reagents for research purposes and/or diagnostic purposes and in medications or medication additives, and to the removal of misfolded proteins/peptides from test reagents for research purposes and/or diagnostic purposes and from medications or medication additives. The invention further relates to detection substances and methods for detecting biofilms, to a method for testing the hemocompatibility of materials and for optimizing medical products, to reagents for cultivating microorganisms in order to make diagnostics and the quality control of biopharmaceuticals easier, and to detection substances for screening for precursors of amyloids, which can be used for technical purposes.

    Abstract translation: 本发明涉及检测和错误折叠的蛋白质/肽的测量领域。 在测定试剂尤其涉及检测和错误折叠的蛋白质/在体液中的肽，在细胞表面和人类和哺乳动物的组织中的测量，该检测错误折叠的蛋白质/肽用于研究和或诊断目的，并且在药物或药品添加剂，以及消除 错误折叠的蛋白质/从检测试剂肽的研究和诊断的目的，或药物或药物添加剂。 此外，本发明包括检测物质和方法用于检测生物膜的，用于测试的材料和医疗产品的优化，试剂的血液相容性微生物的富集，以促进生物制药和检测试剂的诊断和质量控制用于筛选淀粉样蛋白的前体的方法， 其可用于工业目的。

    ISOLATION OF POLYMERASE-NUCLEIC ACID COMPLEXES AND LOADING ONTO SUBSTRATES
    5.
    发明申请
    ISOLATION OF POLYMERASE-NUCLEIC ACID COMPLEXES AND LOADING ONTO SUBSTRATES 审中-公开
    聚合酶 - 核酸复合物的分离和加载到基质上

    公开(公告)号：WO2012129242A2

    公开(公告)日：2012-09-27

    申请号：PCT/US2012/029830

    申请日：2012-03-20

    Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. ,  THANG, Pham ,  GRAY, Jeremy ,  FEHR, Adrian ,  BHAMIDIPATI, Arunashree ,  CHMURA, AJ ,  TRAVERS, Kevin ,  OLIVARES, Eric

    Inventor： THANG, Pham ,  GRAY, Jeremy ,  FEHR, Adrian ,  BHAMIDIPATI, Arunashree ,  CHMURA, AJ ,  TRAVERS, Kevin ,  OLIVARES, Eric

    IPC: C12Q1/48 ,  C12Q1/68 ,  C12N15/11 ,  G01N27/26 ,  G01N33/68

    CPC classification number: C12Q1/6874 ,  B01L9/523 ,  C12Q1/6869 ,  C40B60/14 ,  G01N33/54313 ,  G01N33/54353 ,  G01N2333/922 ,  C12Q2521/543 ,  C12Q2523/303 ,  C12Q2525/301

    Abstract: Compositions, methods and systems are provided for the isolation of polymerase-nucleic acid complexes. Complexes can be separated from free enzyme by using hook molecules to target single stranded regions on the nucleic acid. Active complexes can be isolated from mixtures having both active and inactive complexes by initiating nucleic acid synthesis so as to open up a portion of a double stranded region rendering that region single stranded. Hook molecules are targeted to bind the sequences that are thus exposed. The hook molecules bound to active polymerase-nucleic acid complex are isolated, and the active polymerase-nucleic acid complexes released. Also disclosed are compositions, devices, and methods for loading molecules-of-interest onto a substrate by contacting beads having molecules-of-interest attached to them with the substrate, for example by providing a field that brings the beads into proximity or contact with the substrate and moves the beads with respect to the substrate. Such molecules-of-interest can be deposited onto substrates for single-molecule analysis.

    Abstract translation: 提供了用于分离聚合酶 - 核酸复合物的组合物，方法和系统。 通过使用钩分子靶向核酸上的单链区域，可以将复合物与游离酶分离。 通过启动核酸合成，可以从具有活性和非活性复合物的混合物中分离活性复合物，从而打开双链区域的一部分，使该区域成为单链。 钩分子被靶向以结合因此暴露的序列。 与活性聚合酶 - 核酸复合物结合的钩分子被分离，并且活性聚合酶 - 核酸复合物被释放。 还公开了用于通过将具有与其连接的珠粒与珠粒接触的珠粒与底物接触而将底物加载到底物上的组合物，装置和方法，例如通过提供使珠粒接近或接触 衬底并相对于衬底移动珠子。 这种感兴趣的分子可以沉积在底物上进行单分子分析。

    NUCLEAR PROTEIN EXTRACTION MEDIUM AND METHOD OF USE
    8.
    发明申请
    NUCLEAR PROTEIN EXTRACTION MEDIUM AND METHOD OF USE 审中-公开
    核蛋白提取介质及其使用方法

    公开(公告)号：WO2008057605A3

    公开(公告)日：2008-07-17

    申请号：PCT/US2007023755

    申请日：2007-11-09

    Applicant: QUIDEL CORP ,  WONG PAUL ,  HELMS MIKE

    Inventor： WONG PAUL ,  HELMS MIKE

    IPC: G01N33/68 ,  C07K1/14

    CPC classification number: G01N33/56983 ,  C07K1/145 ,  G01N2333/11 ,  G01N2333/922

    Abstract: An extraction medium for use in detection of a protein in a biological sample is described. The extraction medium contains a combination of reagents, including a nuclease, that aid in nuclear protein extraction from the biological sample. The medium is particularly suited for use in commercially-available rapid diagnostic assays, where the medium improves sensitivity of the assay to the presence of viral nuclear proteins. Kits comprising the extraction medium and methods of using the extraction medium are also described.

    Abstract translation: 描述了用于检测生物样品中的蛋白质的提取介质。 提取介质含有一系列试剂，包括核酸酶，有助于从生物样品中提取核蛋白。 该培养基特别适用于商业上可用的快速诊断测定，其中培养基提高了测定对病毒核蛋白存在的敏感性。 还描述了包含提取介质的试剂盒和使用提取介质的方法。

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