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    METHOD
    3.
    发明申请
    METHOD 审中-公开
    方法

    公开(公告)号：WO2010122531A2

    公开(公告)日：2010-10-28

    申请号：PCT/IB2010/051802

    申请日：2010-04-23

    Applicant: DANISCO A/S ,  MADRID, Susan M ,  LIN, Cherry ,  ZARGAHI, Masoud Rajabi ,  LORENTSEN, Rikke Høegh ,  ISAKSEN, Mai Faurschou ,  WARD, Michael

    Inventor： MADRID, Susan M ,  LIN, Cherry ,  ZARGAHI, Masoud Rajabi ,  LORENTSEN, Rikke Høegh ,  ISAKSEN, Mai Faurschou ,  WARD, Michael

    IPC: C12N9/20

    CPC classification number: C12N9/20 ,  A23K20/189 ,  A23L29/06 ,  A23L33/14 ,  A23V2002/00 ,  C12N15/80 ,  C12Y301/01003

    Abstract: The present invention provides a method of producing a lipolytic enzyme comprising the steps of: (i) providing a Trichoderma reesei cell comprising a) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2 or an amino acid sequence which has at least 40% sequence identity to SEQ ID NO: 1 or 2; and/or b) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4, and/or c) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which has at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4 or the complement of any thereof under stringent conditions; and (ii) culturing the cell under conditions to allow for expression of said heterologous nucleotide sequence(s) encoding said lipolytic enzyme.

    Abstract translation: 本发明提供一种生产脂肪分解酶的方法，包括以下步骤：（i）提供里氏木霉细胞，其包含a）至少一种编码脂解酶的异源核苷酸序列，其包含SEQ ID NO：1所示的氨基酸序列或 SEQ ID NO：2或与SEQ ID NO：1或2具有至少40％序列同一性的氨基酸序列; 和/或b）编码脂解酶的至少一种异源核苷酸序列，其中所述核苷酸序列包含SEQ ID NO：3或SEQ ID NO：4所示的核苷酸序列或与SEQ ID NO：3或SEQ ID NO：4具有至少40％序列同一性的核苷酸序列 SEQ ID NO：3或SEQ ID NO：4，和/或c）至少一个编码脂肪分解酶的异源核苷酸序列，其中所述核苷酸序列包含与SEQ ID NO：3或SEQ ID NO：4杂交的核苷酸序列或 在严格条件下与SEQ ID NO：3或SEQ ID NO：4具有至少40％序列同一性或其任何互补序列的核苷酸序列; 和（ii）在允许表达编码所述脂肪分解酶的所述异源核苷酸序列的条件下培养细胞。

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