METHOD OF PROCESSING POLYNUCLEIC ACIDS
    4.
    发明申请

    公开(公告)号:WO2023084369A1

    公开(公告)日:2023-05-19

    申请号:PCT/IB2022/060640

    申请日:2022-11-04

    摘要: A method of processing polynucleic acids is described comprising: providing a solid support comprising ligands with an amide group; exposing the solid support to polynucleic acid molecules in a buffer having a pH less than 5.5 to bind at least a portion of the polynucleic acid molecules to the ligands; c) exposing the solid support (e.g. particles) with bound polynucleic acid molecules to a buffer having a pH greater than 6 to release a portion of the bound polynucleic acid molecules from the ligands of the particles and to retain a portion of the polynucleic acid molecules bound to the solid support (e.g. particles); and utilizing the solid support with retained bound polynucleic acid molecules or suspension thereof.