公开(公告)号：WO2019055852A1

公开(公告)日：2019-03-21

申请号：PCT/US2018/051183

申请日：2018-09-14

Applicant: APTON BIOSYSTEMS, INC.

Inventor： LIU, Niandong ,  BURNS, Norman ,  FURTADO, Manohar R. ,  STAKER, Bryan, P.

IPC: C12Q1/68 ,  C12Q1/6841 ,  C40B70/00 ,  G01N21/64

CPC classification number: C12Q1/6834 ,  C12Q1/6804 ,  C12Q1/6816 ,  C40B30/04 ,  C40B70/00 ,  G01N33/5008 ,  G01N33/569 ,  G01N33/6845 ,  G01N2021/6439 ,  G01N2458/10 ,  C12Q2563/131 ,  C12Q2563/159 ,  C12Q2565/513 ,  C12Q2565/514 ,  C12Q2565/518

Abstract: Disclosed herein are methods of detecting at least one target biomolecule in at least one single cell comprising lysing the single cell or cells and performing a cell identification assay and target identification assay. Also disclosed herein are methods for preparing a sample for undergoing single cell analysis, wherein the single cell analysis comprises performing a cell identification assay and a target identification assay.

公开(公告)号：WO2018175402A1

公开(公告)日：2018-09-27

申请号：PCT/US2018/023310

申请日：2018-03-20

Applicant: APTON BIOSYSTEMS, INC.

Inventor： FURTADO, Manohar R. ,  FANG, Rixun ,  LIU, Niandong ,  STAKER, Bryan P.

IPC: C12Q1/68

Abstract: The invention relates to methods and compositions for the detection and quantification of nucleotide sequence variants, such as genetic polymorphisms, with decreased error and increased sensitivity, including single molecule detection. Detection of genetic polymorphisms, including single nucleotide polymorphisms (SNPs), is highly useful for the study of physiology, disease, phylogeny and forensics. Current methods for the detection and identification of nucleic acid sequence variants, such as genetic polymorphisms, lack the sensitivity to accurately detect low incidence mutations sequence variants or alleles. Detection techniques for highly multiplexed single molecule identification and quantification of analytes using optical systems are disclosed. Analytes include, but are not limited to, nucleic acid, such as DNA and RNA molecules, with and without modifications. Techniques described herein include use of specific and non-specific probes complementary to nucleic acids of interest for detailed characterization of nucleotide sequence variants and highly multiplexed single molecule identification and quantification.

公开(公告)号：WO2018170518A1

公开(公告)日：2018-09-20

申请号：PCT/US2018/023187

申请日：2018-03-19

Applicant: APTON BIOSYSTEMS, INC.

Inventor： STAKER, Bryan P. ,  LIU, Niandong ,  FURTADO, Manohar R. ,  FANG, Rixun ,  BURNS, Norman ,  OWENS, Windsor

IPC: B01J19/00 ,  C07H21/04 ,  C12Q1/6876

Abstract: Disclosed herein are methods and systems for detection and discrimination of optical signals from a densely packed substrate. These have broad applications for biomolecule detection near or below the diffraction limit of optical systems, including in improving the efficiency and accuracy of polynucleotide sequencing applications.