公开(公告)号：WO2014036238A1

公开(公告)日：2014-03-06

申请号：PCT/US2013/057258

申请日：2013-08-29

Applicant: ATHENIX CORP.

Inventor： SAMPSON, Kimberly, S.

IPC: C12N15/82 ,  C07K14/325

CPC classification number: C12N15/8286 ,  A01N37/46 ,  A01N63/00 ,  C07K14/325 ,  C12N15/8285 ,  Y02A40/162 ,  Y02A40/164

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:3-6, or the nucleotide sequence set forth in SEQ ID NO:1 or 2, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3-6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1或2所示的核苷酸序列，以及变体和 片段。

公开(公告)号：WO2013134523A1

公开(公告)日：2013-09-12

申请号：PCT/US2013/029647

申请日：2013-03-07

Applicant: ATHENIX CORP.

Inventor： SAMPSON, Kimberly, S. ,  THAYER, Rebecca ,  LEHTINEN, Duane

IPC: C07K14/32 ,  C12N15/82

CPC classification number: C07K14/325 ,  A01N37/18 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO: 1 or 3, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2或4所示的氨基酸序列的核苷酸序列或SEQ ID NO：1或3所示的核苷酸序列，以及变体和 片段。

公开(公告)号：WO2013019600A1

公开(公告)日：2013-02-07

申请号：PCT/US2012/048488

申请日：2012-07-27

Applicant: ATHENIX CORP. ,  SAMPSON, Kimberly, S. ,  BALASUBRAMANIAN, Deepa ,  LEHTINEN, Duane, A.

Inventor： SAMPSON, Kimberly, S. ,  BALASUBRAMANIAN, Deepa ,  LEHTINEN, Duane, A.

IPC: C12N15/82 ,  C07K14/325 ,  A01H5/00

CPC classification number: C12N15/8286 ,  A01H5/10 ,  A01H5/12 ,  C07K14/195 ,  Y02A40/162

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 3, or 4, the nucleotide sequence set forth in SEQ ID NO: 1, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2,3或4所示氨基酸序列，SEQ ID NO：1所示核苷酸序列以及变体和片段的核苷酸序列的核酸分子 它们。

公开(公告)号：WO2015038262A2

公开(公告)日：2015-03-19

申请号：PCT/US2014/050316

申请日：2014-08-08

Applicant: ATHENIX CORP.

Inventor： THAYER, Rebecca ,  SAMPSON, Kimberly, S. ,  LEHTINEN, Duane ,  PETERS, Cheryl

IPC: C07K14/325

CPC classification number: C12N15/8286 ,  A01N63/02 ,  C07K14/325 ,  Y02A40/162

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2-5, or the nucleotide sequence set forth in SEQ ID NO: 1, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物和细菌中转化和表达。 组合物还包含转化的细菌，植物，植物细胞，组织和种子。 具体而言，提供了分离的毒素核酸分子。 此外，还包括对应于多核苷酸的氨基酸序列，以及与那些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2-5所示的氨基酸序列或SEQ ID NO：1所示的核苷酸序列的核苷酸序列，以及其变体和片段

公开(公告)号：WO2012135501A2

公开(公告)日：2012-10-04

申请号：PCT/US2012/031214

申请日：2012-03-29

Applicant: ATHENIX CORP. ,  SAMPSON, Kimberly, S.

Inventor： SAMPSON, Kimberly, S.

IPC: C12N15/82 ,  C07K14/325

CPC classification number: C12N15/8286 ,  A01N63/02 ,  C07K14/325 ,  C12N15/8261 ,  Y02A40/146 ,  Y02A40/162

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4-19, or the nucleotide sequence set forth in SEQ ID NO: 1-3, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物和细菌中转化和表达。 组合物还包含转化的细菌，植物，植物细胞，组织和种子。 具体而言，提供了分离的毒素核酸分子。 此外，还包括对应于多核苷酸的氨基酸序列，以及与那些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：4-19所示氨基酸序列或SEQ ID NO：1-3所示核苷酸序列的核苷酸序列，以及变体和 片段。

公开(公告)号：WO2015041769A2

公开(公告)日：2015-03-26

申请号：PCT/US2014/050327

申请日：2014-08-08

Applicant: ATHENIX CORP.

Inventor： THAYER, Rebecca ,  SAMPSON, Kimberly, S. ,  LEHTINEN, Duane ,  MAGALHAES, Leonardo

IPC: A01H5/00 ,  C12N15/82

CPC classification number: C12N15/8286 ,  C07K14/325

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:3-6, or the nucleotide sequence set forth in SEQ ID NO: 1 or 2, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3-6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1或2所示的核苷酸序列，以及变体和 片段。

公开(公告)号：WO2015021367A2

公开(公告)日：2015-02-12

申请号：PCT/US2014/050319

申请日：2014-08-08

Applicant: ATHENIX CORP.

Inventor： ROBERTS, Kira ,  SAMPSON, Kimberly, S. ,  LEHTINEN, Duane ,  MAGALHAES, Leanardo

IPC: A01H5/00 ,  C12N15/82

CPC classification number: C12N15/8286 ,  C07K14/325 ,  Y02A40/162

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2-3, or the nucleotide sequence set forth in SEQ ID NO: l, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物和细菌中转化和表达。 组合物还包含转化的细菌，植物，植物细胞，组织和种子。 具体而言，提供了分离的毒素核酸分子。 此外，还包括对应于多核苷酸的氨基酸序列，以及与那些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2-3所示的氨基酸序列或SEQ ID NO：1所示的核苷酸序列的核苷酸序列，以及其变体和片段

公开(公告)号：WO2011103248A2

公开(公告)日：2011-08-25

申请号：PCT/US2011/025172

申请日：2011-02-17

Applicant: ATHENIX CORP. ,  SAMPSON, Kimberly, S. ,  TOMSO, Daniel, John

Inventor： SAMPSON, Kimberly, S. ,  TOMSO, Daniel, John

IPC: A01H5/00 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12N5/10 ,  C07K14/00 ,  A01N37/18 ,  C12P21/00 ,  A01H1/00 ,  C12N15/82 ,  A01H5/10 ,  A01P7/04

CPC classification number: C12N15/8286 ,  A01N37/46 ,  A01N63/02 ,  C07K14/325 ,  C07K16/1278 ,  C12N15/8279 ,  Y02A40/162

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO :21-32, or the nucleotide sequence set forth in SEQ ID NO: 1-20, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：21-32所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-20所示的核苷酸序列，以及变体和 片段。

公开(公告)号：WO2011002992A1

公开(公告)日：2011-01-06

申请号：PCT/US2010/040778

申请日：2010-07-01

Applicant: ATHENIX CORP. ,  DESAI, Nalini ,  HINSON, Jill ,  BALUSUBRAMANIAN, Deepa ,  SAMPSON, Kimberly, S. ,  TOMSO, Daniel, J. ,  LEHTINEN, Duane, Alan ,  DUCK, Nicholas, B.

Inventor： DESAI, Nalini ,  HINSON, Jill ,  BALUSUBRAMANIAN, Deepa ,  SAMPSON, Kimberly, S. ,  TOMSO, Daniel, J. ,  LEHTINEN, Duane, Alan ,  DUCK, Nicholas, B.

IPC: C12N15/82

CPC classification number: C07K14/195 ,  C07K14/22 ,  C07K16/1203 ,  C12N15/8285 ,  C12N15/8286 ,  C12Q1/6876 ,  Y02A40/162 ,  Y02A40/164

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 3, or 4, the nucleotide sequence set forth in SEQ ID NO: 1, 9, 10, or 11, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2,3或4所示氨基酸序列的核苷酸序列的核酸分子，SEQ ID NO：1,9,10或11所示的核苷酸序列 ，以及其变体和片段。

公开(公告)号：WO2015088937A8

公开(公告)日：2015-06-18

申请号：PCT/US2014/068989

申请日：2014-12-08

Applicant: ATHENIX CORP.

Inventor： LEHTINEN, Duane Alan ,  SAMPSON, Kimberly, S. ,  ROBERTS, Kira ,  DUNN, Ethan ,  CHOUGULE, Nana

IPC: C12N15/82 ,  C07K14/325

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a Bacillus thuringiensis toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:5-26, or the nucleotide sequence set forth in SEQ ID NO: 1-4, as well as variants and fragments thereof.