公开(公告)号：WO2015041769A2

公开(公告)日：2015-03-26

申请号：PCT/US2014/050327

申请日：2014-08-08

Applicant: ATHENIX CORP.

Inventor： THAYER, Rebecca ,  SAMPSON, Kimberly, S. ,  LEHTINEN, Duane ,  MAGALHAES, Leonardo

IPC: A01H5/00 ,  C12N15/82

CPC classification number: C12N15/8286 ,  C07K14/325

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:3-6, or the nucleotide sequence set forth in SEQ ID NO: 1 or 2, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3-6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1或2所示的核苷酸序列，以及变体和 片段。

公开(公告)号：WO2015021367A2

公开(公告)日：2015-02-12

申请号：PCT/US2014/050319

申请日：2014-08-08

Applicant: ATHENIX CORP.

Inventor： ROBERTS, Kira ,  SAMPSON, Kimberly, S. ,  LEHTINEN, Duane ,  MAGALHAES, Leanardo

IPC: A01H5/00 ,  C12N15/82

CPC classification number: C12N15/8286 ,  C07K14/325 ,  Y02A40/162

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2-3, or the nucleotide sequence set forth in SEQ ID NO: l, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物和细菌中转化和表达。 组合物还包含转化的细菌，植物，植物细胞，组织和种子。 具体而言，提供了分离的毒素核酸分子。 此外，还包括对应于多核苷酸的氨基酸序列，以及与那些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2-3所示的氨基酸序列或SEQ ID NO：1所示的核苷酸序列的核苷酸序列，以及其变体和片段

公开(公告)号：WO2014138339A2

公开(公告)日：2014-09-12

申请号：PCT/US2014/021021

申请日：2014-03-06

Applicant: ATHENIX CORP. ,  BAYER CROPSCIENCE LP

Inventor： THAYER, Rebecca ,  ROBERTS, Kira ,  SAMPSON, Kimberly ,  LEHTINEN, Duane ,  PETERS, Cheryl ,  MAGALHAES, Leonardo ,  DUNN, Ethan

IPC: C12N15/82

CPC classification number: C12N15/8286 ,  A01N37/46 ,  A01N63/02 ,  C07K14/325 ,  Y02A40/162

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:21-74, or the nucleotide sequence set forth in SEQ ID NO: 1-20, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物和细菌中转化和表达。 组合物还包含转化的细菌，植物，植物细胞，组织和种子。 具体而言，提供了分离的毒素核酸分子。 此外，还包括对应于多核苷酸的氨基酸序列，以及与那些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：21-74所示的氨基酸序列或SEQ ID NO：1-20所示的核苷酸序列的核苷酸序列，以及变体和 片段。

公开(公告)号：WO2014003769A1

公开(公告)日：2014-01-03

申请号：PCT/US2012/044751

申请日：2012-06-29

Applicant: ATHENIX CORP. ,  POUTRE, Candace ,  KAHN, Theodore, W. ,  ANTHONY, Alissa

Inventor： POUTRE, Candace ,  KAHN, Theodore, W. ,  ANTHONY, Alissa

IPC: C12N15/82 ,  C07K14/325

CPC classification number: C12N15/8286 ,  C07K14/325 ,  C12N15/8285 ,  Y02A40/162 ,  Y02A40/164

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 3, or the nucleotide sequence set forth in SEQ ID NO: l, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2或3所示的氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列，以及其变体和片段 。

公开(公告)号：WO2013134535A1

公开(公告)日：2013-09-12

申请号：PCT/US2013/029666

申请日：2013-03-07

Applicant: ATHENIX CORP.

Inventor： SAMPSON, Kimberly ,  THAYER, Rebecca ,  LEHTINEN, Duane

IPC: C07K14/325

CPC classification number: C12N15/8286 ,  C07K14/325

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO: 1 or 3, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2所示的氨基酸序列的核苷酸序列或SEQ ID NO：1或3所示的核苷酸序列，以及其变体和片段 。

公开(公告)号：WO2011103248A2

公开(公告)日：2011-08-25

申请号：PCT/US2011/025172

申请日：2011-02-17

Applicant: ATHENIX CORP. ,  SAMPSON, Kimberly, S. ,  TOMSO, Daniel, John

Inventor： SAMPSON, Kimberly, S. ,  TOMSO, Daniel, John

IPC: A01H5/00 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12N5/10 ,  C07K14/00 ,  A01N37/18 ,  C12P21/00 ,  A01H1/00 ,  C12N15/82 ,  A01H5/10 ,  A01P7/04

CPC classification number: C12N15/8286 ,  A01N37/46 ,  A01N63/02 ,  C07K14/325 ,  C07K16/1278 ,  C12N15/8279 ,  Y02A40/162

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO :21-32, or the nucleotide sequence set forth in SEQ ID NO: 1-20, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：21-32所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-20所示的核苷酸序列，以及变体和 片段。

公开(公告)号：WO2011002992A1

公开(公告)日：2011-01-06

申请号：PCT/US2010/040778

申请日：2010-07-01

Applicant: ATHENIX CORP. ,  DESAI, Nalini ,  HINSON, Jill ,  BALUSUBRAMANIAN, Deepa ,  SAMPSON, Kimberly, S. ,  TOMSO, Daniel, J. ,  LEHTINEN, Duane, Alan ,  DUCK, Nicholas, B.

Inventor： DESAI, Nalini ,  HINSON, Jill ,  BALUSUBRAMANIAN, Deepa ,  SAMPSON, Kimberly, S. ,  TOMSO, Daniel, J. ,  LEHTINEN, Duane, Alan ,  DUCK, Nicholas, B.

IPC: C12N15/82

CPC classification number: C07K14/195 ,  C07K14/22 ,  C07K16/1203 ,  C12N15/8285 ,  C12N15/8286 ,  C12Q1/6876 ,  Y02A40/162 ,  Y02A40/164

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 3, or 4, the nucleotide sequence set forth in SEQ ID NO: 1, 9, 10, or 11, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2,3或4所示氨基酸序列的核苷酸序列的核酸分子，SEQ ID NO：1,9,10或11所示的核苷酸序列 ，以及其变体和片段。

公开(公告)号：WO2009049126A3

公开(公告)日：2009-06-25

申请号：PCT/US2008079478

申请日：2008-10-10

Applicant: ATHENIX CORP ,  CAROZZI NADINE ,  DESAI NALINI ,  TOMSO DANIEL J

Inventor： CAROZZI NADINE ,  DESAI NALINI ,  TOMSO DANIEL J

IPC: C12N15/82

CPC classification number: C12N15/8286 ,  C07K14/325 ,  Y02A40/162

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions containing a synthetic nucleotide sequence encoding a Cryl Ac protein are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided, wherein the nucleotide sequences are set forth in SEQ ID NO:5, 1, 2, 3, or 4, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了含有编码Cry1Ac蛋白质的合成核苷酸序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子，其中核苷酸序列如SEQ ID NO：5,1,2,3或4所示，以及其变体和片段。

公开(公告)号：WO2008100353A2

公开(公告)日：2008-08-21

申请号：PCT/US2007085164

申请日：2007-11-20

Applicant: ATHENIX CORP ,  SCHOUTEN LAURA COOPER ,  PETERS CHERYL ,  VANDE BERG BRIAN ,  HINSON TODD

Inventor： SCHOUTEN LAURA COOPER ,  PETERS CHERYL ,  VANDE BERG BRIAN ,  HINSON TODD

IPC: C12N15/82

CPC classification number: C12N15/8275 ,  C12N9/1085 ,  C12N9/1092

Abstract: Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds.. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34.

Abstract translation: 提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。 组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供编码抗草甘膦或多肽的分离的多核苷酸。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的多核苷酸，其含有编码SEQ ID NO：9,11,13,15,17,19,21,23,25,27,29,31,33， 或35，或SEQ ID NO：6,8,10,12,14,16,18,20,22,24,26,28,30,32或34所示的核苷酸序列。

公开(公告)号：WO2006083891A3

公开(公告)日：2006-11-30

申请号：PCT/US2006003434

申请日：2006-01-31

Applicant: ATHENIX CORP ,  CAROZZI NADINE ,  HARGISS TRACY ,  KOZIEL MICHAEL G ,  DUCK NICHOLAS B

Inventor： CAROZZI NADINE ,  HARGISS TRACY ,  KOZIEL MICHAEL G ,  DUCK NICHOLAS B

IPC: C07K14/325 ,  A01N63/02 ,  C12N15/00

CPC classification number: C07K14/325 ,  A01N63/02 ,  C12N15/8281 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID N0: 2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO: 1, 3, or 5, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,4或6所示氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列 ，以及其变体和片段。