公开(公告)号：WO2021133649A8

公开(公告)日：2021-07-01

申请号：PCT/US2020/065825

申请日：2020-12-18

Applicant: BECKMAN COULTER, INC.

Inventor： FRIESEN, Taylor A. ,  LU, Kristina K. ,  SAUERBURGER, Mark F. ,  SMITH, Zachary M. ,  WILSON, Jeffrey J.

IPC: G01N35/10 ,  G01N35/00 ,  B01L3/00 ,  C12Q1/00 ,  B01L2300/0851 ,  B01L3/52 ,  G01N35/0099 ,  G01N35/1002 ,  G01N35/1011 ,  G01N35/1016 ,  G01N35/1065

Abstract: Described herein are method of transferring liquid using a robotic liquid handier from a reagent reservoir having a sloped bottom along a length of the reagent reservoir, the sloped bottom defining a shallow end and a deep end of the reagent reservoir, wherein the shallow end is proximal to a first side-wall of the reagent reservoir, wherein the deep end is proximal to a second side-wall of the reagent reservoir opposite the first side-wall.

公开(公告)号：WO2021127315A1

公开(公告)日：2021-06-24

申请号：PCT/US2020/065810

申请日：2020-12-18

Applicant: BECKMAN COULTER, INC.

Inventor： DAVIS, Matthew S. ,  LU, Kristina K. ,  MOSCHELL, Rachel Ellen ,  NEI, Peter Robert ,  SAUERBURGER, Mark F ,  SMITH, Zachary M. ,  SNIDER, John S. ,  SPRINGSTON, Jason L.

IPC: B01L3/00 ,  C12Q1/6806 ,  C12Q1/6844 ,  G01N35/00 ,  B01L2200/023 ,  B01L2200/147 ,  B01L2300/021 ,  B01L2300/0858 ,  B01L2300/1822 ,  B01L2400/043 ,  B01L3/5082 ,  B01L7/54 ,  B01L9/06 ,  C12Q1/6846

Abstract: A reaction vessel comprises a lower chamber with a first volume, and an upper chamber with a second volume greater than the first volume. A thermocycling system for heating the reaction vessel includes a lower heating zone to heat the lower chamber, an upper heating zone to heat the upper chamber, and a lid heater to heat an opening of the upper chamber. A method comprises loading a sample into a lower chamber of a reaction vessel, thermocycling the lower chamber using a lower heating zone of the thermo cycler, combining an additive into the sample to produce a combination filling the lower chamber and at least partially filling an upper chamber of the reaction vessel, and incubating the upper and lower chambers using the lower heating zone and an upper heating zone. The lower and upper chambers can have different wall thicknesses to facilitate heat transfer.

公开(公告)号：WO2021133649A1

公开(公告)日：2021-07-01

申请号：PCT/US2020/065825

申请日：2020-12-18

Applicant: BECKMAN COULTER, INC.

Inventor： FRIESEN, Taylor A. ,  LU, Kristina K. ,  SAUERBURGER, Mark F. ,  SMITH, Zachary M. ,  WILSON, Jeffrey J.

IPC: G01N35/10 ,  G01N35/00 ,  B01L3/00 ,  C12Q1/00 ,  B01L2300/0851 ,  B01L3/52 ,  G01N35/0099 ,  G01N35/1002 ,  G01N35/1011 ,  G01N35/1016 ,  G01N35/1065

Abstract: Described herein are method of transferring liquid using a robotic liquid handier from a reagent reservoir having a sloped bottom along a length of the reagent reservoir, the sloped bottom defining a shallow end and a deep end of the reagent reservoir, wherein the shallow end is proximal to a first side-wall of the reagent reservoir, wherein the deep end is proximal to a second side-wall of the reagent reservoir opposite the first side-wall.