公开(公告)号：WO2022011261A2

公开(公告)日：2022-01-13

申请号：PCT/US2021/041088

申请日：2021-07-09

Applicant: DETECT, INC.

Inventor： GLANTZ, Spencer ,  ROTHBERG, Jonathan, M. ,  KEMBLE, Henry ,  SABINA, Jeff ,  JARAMILLO-RIVERI, Sebastian

IPC: C12Q1/6853 ,  C07H21/04 ,  C12Q1/6832 ,  C12Q1/6818 ,  C12Q1/6844 ,  C12Q1/6846 ,  C12Q1/6888 ,  C12Q2600/158

Abstract: Aspects of the disclosure relate to compositions and methods for amplifying and/or detecting one or more target nucleic acid sequences (e.g., a nucleic acid sequence of one or more pathogens) in a biological sample obtained from a subject. In some embodiments, the pathogens are viral, bacterial, fungal, parasitic, or protozoan pathogens, such as SARS-CoV-2 or an influenza virus. In some embodiments, the methods comprise isothermal amplification of a target nucleic acid and subsequent detection of the amplification products.

公开(公告)号：WO2022200213A1

公开(公告)日：2022-09-29

申请号：PCT/EP2022/057172

申请日：2022-03-18

Applicant: VPCIR.COM APS

Inventor： TESAURO, Cinzia ,  STOUGAARD, Magnus ,  KNUDSEN, Birgitta R.

IPC: C12Q1/533 ,  C12Q1/6844 ,  C12Q1/6846

Abstract: The present invention relates to assays for determining the influence of an anti-topoisomerase 1B drug on the cleavage step and/or the ligation step of topoisomerase 1B enzymatic activity. The invention also relates to kits comprising such assays and uses thereof.

公开(公告)号：WO2022189645A1

公开(公告)日：2022-09-15

申请号：PCT/EP2022/056361

申请日：2022-03-11

Applicant: CHEMOMETEC A/S

Inventor： TROELSGAARD PEDERSEN, Rune ,  KJÆRULFF, Søren ,  HANSVILLEMENKE, Alfred

IPC: C12Q1/6844 ,  C12Q1/6846

Abstract: Detection and analysis of biological particles can provide useful information with respect to a wide range of applications. Those include but are not limited to analysis of disease markers in diagnostic samples, quantification of bacteria and/or viral particles in environmental samples, and detection of specific proteins in cell culture samples. The methods presented herein provide simple, rapid and reliable means of detecting and analyzing biological particles in a hydrogel, and are especially suitable for quantification of for example nucleic acids, viral particles, bacteria, yeast, animal cells, and proteins. The invention also relates to a kit of parts and to a system for detection and analysis of biological particles in a hydrogel.

公开(公告)号：WO2021127315A1

公开(公告)日：2021-06-24

申请号：PCT/US2020/065810

申请日：2020-12-18

Applicant: BECKMAN COULTER, INC.

Inventor： DAVIS, Matthew S. ,  LU, Kristina K. ,  MOSCHELL, Rachel Ellen ,  NEI, Peter Robert ,  SAUERBURGER, Mark F ,  SMITH, Zachary M. ,  SNIDER, John S. ,  SPRINGSTON, Jason L.

IPC: B01L3/00 ,  C12Q1/6806 ,  C12Q1/6844 ,  G01N35/00 ,  B01L2200/023 ,  B01L2200/147 ,  B01L2300/021 ,  B01L2300/0858 ,  B01L2300/1822 ,  B01L2400/043 ,  B01L3/5082 ,  B01L7/54 ,  B01L9/06 ,  C12Q1/6846

Abstract: A reaction vessel comprises a lower chamber with a first volume, and an upper chamber with a second volume greater than the first volume. A thermocycling system for heating the reaction vessel includes a lower heating zone to heat the lower chamber, an upper heating zone to heat the upper chamber, and a lid heater to heat an opening of the upper chamber. A method comprises loading a sample into a lower chamber of a reaction vessel, thermocycling the lower chamber using a lower heating zone of the thermo cycler, combining an additive into the sample to produce a combination filling the lower chamber and at least partially filling an upper chamber of the reaction vessel, and incubating the upper and lower chambers using the lower heating zone and an upper heating zone. The lower and upper chambers can have different wall thicknesses to facilitate heat transfer.