公开(公告)号：WO2009086116A3

公开(公告)日：2009-10-08

申请号：PCT/US2008087696

申请日：2008-12-19

Applicant: CENTOCOR INC ,  JACOBS STEVEN

Inventor： JACOBS STEVEN

IPC: C12N15/00

CPC classification number: C40B40/02 ,  C12N15/1037 ,  C12N15/1044

Abstract: The invention relates to compositions and methods for generating and using plX phage display libraries for producing non-antibody scaffold protein fusions using plX of M13 phage.

Abstract translation: 本发明涉及用于产生和使用plX噬菌体展示文库用于使用M13噬菌体的plX产生非抗体支架蛋白融合物的组合物和方法。

公开(公告)号：WO2010051310A2

公开(公告)日：2010-05-06

申请号：PCT/US2009062367

申请日：2009-10-28

Applicant: CENTOCOR ORTHO BIOTECH INC ,  JACOBS STEVEN ,  O'NEIL KARYN

Inventor： JACOBS STEVEN ,  O'NEIL KARYN

IPC: A61K38/39 ,  C12N15/12 ,  C12N15/70 ,  C40B30/04 ,  C40B40/10

CPC classification number: G01N33/54306 ,  A61K38/39 ,  C07K14/78

Abstract: A protein scaffold based on a consensus sequence of the tenth f?bronectin type III (FN3) repeat from human f?bronectin, preferably human Tenascin, that binds to human TNFa including isolated nucleic acids that encode a protein scaffold, vectors, host cells, and methods of making and using thereof have applications in diagnostic and/or therapeutic compositions, methods and devices.

Abstract translation: 一种蛋白质支架，其基于来自人fβ支链淀粉（优选人腱生蛋白）的第十种β链球菌III型（FN3）重复序列的共有序列，其结合人TNFα，包括编码蛋白质支架，载体，宿主细胞的分离的核酸， 其制备和使用方法具有在诊断和/或治疗组合物，方法和装置中的应用。

公开(公告)号：WO2011137319A3

公开(公告)日：2012-01-19

申请号：PCT/US2011034512

申请日：2011-04-29

Applicant: CENTOCOR ORTHO BIOTECH INC ,  JACOBS STEVEN

Inventor： JACOBS STEVEN

IPC: A61K49/00 ,  A61K38/16 ,  A61K38/22 ,  A61P1/00 ,  A61P7/00 ,  A61P9/00 ,  A61P11/00 ,  A61P11/02 ,  A61P25/00 ,  A61P27/02 ,  A61P27/16 ,  A61P31/00 ,  A61P35/00 ,  A61P37/02 ,  A61P43/00 ,  C07H21/00 ,  C07K14/00 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/63 ,  C40B30/04 ,  C40B40/02 ,  C40B40/08 ,  C40B50/00

CPC classification number: C12N15/1044 ,  A61K38/00 ,  C07K14/78 ,  C07K16/18 ,  C07K2318/20

Abstract: A protein scaffold based on a consensus sequence of fibronectin type III (FN3) proteins, such as the tenth FN3 repeat from human fibronectin (human Tenascin), including isolated nucleic acids that encode a protein scaffold, vectors, host cells, and methods of making and using thereof. The protein scaffold molecules of the present invention exhibit enhanced thermal and chemical stability while presenting six modifiable loop domains which can be engineered to form a binding partner capable of binding to a target for applications in diagnostic and/or therapeutic compositions, methods and devices.

Abstract translation: 基于纤连蛋白III（FN3）蛋白的共有序列的蛋白质支架，例如来自人纤连蛋白（人腱生蛋白）的第十FN3重复序列，包括编码蛋白质支架的分离的核酸，载体，宿主细胞和制备方法 并使用它们。 本发明的蛋白质支架分子表现出增强的热和化学稳定性，同时呈现六个可修饰的环结构域，其可被工程化以形成能够与诊断和/或治疗组合物，方法和装置中的应用结合的靶标的结合配偶体。

公开(公告)号：WO2010051274A3

公开(公告)日：2010-09-10

申请号：PCT/US2009062200

申请日：2009-10-27

Applicant: CENTOCOR ORTHO BIOTECH INC ,  JACOBS STEVEN ,  O'NEIL KARYN

Inventor： JACOBS STEVEN ,  O'NEIL KARYN

IPC: C07K16/00

CPC classification number: C07K14/78 ,  C40B40/08 ,  C40B50/06

Abstract: A protein scaffold based on a consensus sequence of f?bronectin type III (FN3) proteins, such as the tenth FN3 repeat from human f?bronectin (human Tenascin), including isolated nucleic acids that encode a protein scaffold, vectors, host cells, and methods of making and using thereof have applications in diagnostic and/or therapeutic compositions, methods and devices. In particular, protein scaffold molecules binding to IgG have been identified as useful for diagnostic and/or therapeutic applications.

Abstract translation: 基于纤连蛋白III型（FN3）蛋白的共有序列的蛋白质支架，如来自人纤连蛋白（人腱生蛋白）的第十个FN3重复序列，包括编码蛋白质支架的分离的核酸，载体，宿主细胞， 及其制备和使用方法可用于诊断和/或治疗组合物，方法和装置。 具体而言，与IgG结合的蛋白质支架分子已被鉴定为可用于诊断和/或治疗应用。

公开(公告)号：WO2010093627A3

公开(公告)日：2010-10-14

申请号：PCT/US2010023625

申请日：2010-02-09

Applicant: CENTOCOR ORTHO BIOTECH INC ,  JACOBS STEVEN ,  O'NEIL KARYN

Inventor： JACOBS STEVEN ,  O'NEIL KARYN

IPC: A61K35/14 ,  A61K38/04

CPC classification number: C12N15/1044 ,  A61K38/00 ,  C07K14/78 ,  C40B40/08 ,  C40B50/06

Abstract: A protein scaffold based on a consensus sequence of the tenth fibronectin type III (FN3) repeat from human fibronectin, including isolated nucleic acids that encode a protein scaffold, vectors, host cells, and methods of making and using thereof have applications in diagnostic and/or therapeutic compositions, methods and devices. In particular, protein scaffold molecules binding to IgG bassed on the consensus sequence have been identified as useful for diagnostic and/or therapeutic applications.

Abstract translation: 基于来自人纤连蛋白的第十纤连蛋白III型（FN3）重复序列的共有序列的蛋白质支架，包括编码蛋白质支架的分离的核酸，载体，宿主细胞及其制备和使用方法，具有诊断和/ 或治疗组合物，方法和装置。 特别是，基于共有序列的与IgG结合的蛋白质支架分子已被鉴定为可用于诊断和/或治疗应用。