公开(公告)号：WO2011137319A3

公开(公告)日：2012-01-19

申请号：PCT/US2011034512

申请日：2011-04-29

Applicant: CENTOCOR ORTHO BIOTECH INC ,  JACOBS STEVEN

Inventor： JACOBS STEVEN

IPC: A61K49/00 ,  A61K38/16 ,  A61K38/22 ,  A61P1/00 ,  A61P7/00 ,  A61P9/00 ,  A61P11/00 ,  A61P11/02 ,  A61P25/00 ,  A61P27/02 ,  A61P27/16 ,  A61P31/00 ,  A61P35/00 ,  A61P37/02 ,  A61P43/00 ,  C07H21/00 ,  C07K14/00 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/63 ,  C40B30/04 ,  C40B40/02 ,  C40B40/08 ,  C40B50/00

CPC classification number: C12N15/1044 ,  A61K38/00 ,  C07K14/78 ,  C07K16/18 ,  C07K2318/20

Abstract: A protein scaffold based on a consensus sequence of fibronectin type III (FN3) proteins, such as the tenth FN3 repeat from human fibronectin (human Tenascin), including isolated nucleic acids that encode a protein scaffold, vectors, host cells, and methods of making and using thereof. The protein scaffold molecules of the present invention exhibit enhanced thermal and chemical stability while presenting six modifiable loop domains which can be engineered to form a binding partner capable of binding to a target for applications in diagnostic and/or therapeutic compositions, methods and devices.

Abstract translation: 基于纤连蛋白III（FN3）蛋白的共有序列的蛋白质支架，例如来自人纤连蛋白（人腱生蛋白）的第十FN3重复序列，包括编码蛋白质支架的分离的核酸，载体，宿主细胞和制备方法 并使用它们。 本发明的蛋白质支架分子表现出增强的热和化学稳定性，同时呈现六个可修饰的环结构域，其可被工程化以形成能够与诊断和/或治疗组合物，方法和装置中的应用结合的靶标的结合配偶体。

公开(公告)号：WO2011150061A1

公开(公告)日：2011-12-01

申请号：PCT/US2011/037904

申请日：2011-05-25

Applicant: CENTOCOR ORTHO BIOTECH INC. ,  DIEM, Michael ,  O'NEIL, Karen, T.

Inventor： DIEM, Michael ,  O'NEIL, Karen, T.

IPC: A61K38/00 ,  A61K39/00 ,  C12P21/06 ,  C12P21/04

CPC classification number: C07K7/08 ,  A61K38/00 ,  C07K14/47 ,  C07K14/7155 ,  C07K2319/02 ,  C07K2319/21 ,  C07K2319/30 ,  C07K2319/61 ,  C07K2319/70

Abstract: Disclosed are insulin-like growth factor 1 receptor binding polypeptides, and the polynucleotides encoding the polypeptide. Disclosed are also methods of producing the polypeptides using cell expression systems and cell free systems, and methods of using the polypeptides for fusion protein production. The polypeptides disclosed can provide means for delivering therapeutic agents across the blood-brain barrier (BBB).

Abstract translation: 公开了胰岛素样生长因子1受体结合多肽，以及编码该多肽的多核苷酸。 还公开了使用细胞表达系统和无细胞系统产生多肽的方法，以及使用该多肽进行融合蛋白生产的方法。 所公开的多肽可提供递送治疗剂穿过血脑屏障（BBB）的方法。

公开(公告)号：WO2011143299A2

公开(公告)日：2011-11-17

申请号：PCT/US2011/036043

申请日：2011-05-11

Applicant: CENTOCOR ORTHO BIOTECH INC. ,  XU, Jean

Inventor： XU, Jean

IPC: C12N5/071 ,  C12Q1/68 ,  C12N5/0735 ,  C12N5/02 ,  C07K14/47 ,  C07K14/50

CPC classification number: C12N5/0678 ,  A61K35/12 ,  C12N5/0606 ,  C12N5/0676 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2506/02

Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce a population of cells expressing markers characteristic of the pancreatic endoderm lineage, wherein greater than 50% of the cells in the population co-express PDX1 and NKX6.1.

Abstract translation: 本发明提供促进多能干细胞分化成胰岛素生成细胞的方法。 特别地，本发明提供了一种产生表达胰腺内胚层谱系特征性标志物的细胞群的方法，其中大于50％的群体中的细胞共表达PDX1和NKX6.1。

公开(公告)号：WO2011059660A3

公开(公告)日：2011-10-20

申请号：PCT/US2010053693

申请日：2010-10-22

Applicant: CENTOCOR ORTHO BIOTECH INC ,  FRYER BENJAMIN

Inventor： FRYER BENJAMIN

IPC: C12N5/02 ,  C12M3/00 ,  C12N5/07 ,  C12N5/0735

CPC classification number: C12N5/0606 ,  C12N2501/70 ,  C12N2509/00 ,  C12N2533/00

Abstract: The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.

Abstract translation: 哺乳动物细胞培养领域本发明涉及哺乳动物细胞培养领域，并且提供了用于细胞附着，在固体基质表面上培养和脱落的方法和组合物，其含有至少约0.5％的N，O和N之和大于或等于 等于17.2％，并且接触角至少约为13.9度，缺乏饲养细胞层并且没有吸附层。 在本发明的一个实施方案中，用能够抑制Rho激酶活性的化合物处理细胞。 在另一个实施方案中，用能够抑制Rho活性的化合物处理细胞。

公开(公告)号：WO2011109279A2

公开(公告)日：2011-09-09

申请号：PCT/US2011/026443

申请日：2011-02-28

Applicant: CENTOCOR ORTHO BIOTECH INC. ,  KARANU, Francis ,  REZANIA, Alireza

Inventor： KARANU, Francis ,  REZANIA, Alireza

IPC: A61K35/39

CPC classification number: C12N5/0676 ,  A61K35/39 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2506/02

Abstract: The present invention is directed to methods to differentiate pluripotent stem cells. In particular, the present invention provides methods of characterization of cells differentiated into cells expressing markers characteristic of the pancreatic endocrine lineage utilizing unique surface markers. The present invention also provides methods to enrich or sort cells expressing markers characteristic of the pancreatic endocrine lineage. The present invention also provides methods to deplete cells that may contaminate populations of cells expressing markers characteristic of the pancreatic endocrine lineage formed by the methods of the present invention, thereby reducing the incidence of tumor formation in vivo following transplantation.

Abstract translation: 本发明涉及分化多能干细胞的方法。 特别地，本发明提供了利用独特的表面标记表征分化成表达胰腺内分泌谱系特征性标志物的细胞的细胞的方法。 本发明还提供了富集或分选表达胰腺内分泌谱系特征性标志物的细胞的方法。 本发明还提供了可以污染可能污染由本发明的方法形成的表达胰腺内分泌谱系特征性标志物的细胞群的细胞的方法，从而降低移植后体内肿瘤形成的发生率。

公开(公告)号：WO2011066501A1

公开(公告)日：2011-06-03

申请号：PCT/US2010/058188

申请日：2010-11-29

Applicant: CENTOCOR ORTHO BIOTECH INC. ,  STROHL, William ,  VAFA, Omid

Inventor： STROHL, William ,  VAFA, Omid

IPC: A61K39/00 ,  C12P21/08

CPC classification number: C07K16/32 ,  A61K38/00 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/2887 ,  C07K2317/52 ,  C07K2317/524 ,  C07K2317/54 ,  C07K2317/71 ,  C07K2317/73 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2317/92

Abstract: Antibody and other Fc-containing molecules with variations in the Fc region reduce binding to Fc gamma receptors and resulting activity and can be used in the treatment of various diseases and disorders.

Abstract translation: 具有Fc区变异的抗体和其它含Fc的分子减少与Fcγ受体的结合并导致其活性，并可用于治疗各种疾病和病症。

公开(公告)号：WO2011059725A2

公开(公告)日：2011-05-19

申请号：PCT/US2010/054408

申请日：2010-10-28

Applicant: CENTOCOR ORTHO BIOTECH INC. ,  FUNG, Ramie ,  FRYER, Benjamin

Inventor： FUNG, Ramie ,  FRYER, Benjamin

CPC classification number: C12N5/0696 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/605 ,  C12N2501/608 ,  C12N2501/70 ,  C12N2502/13 ,  C12N2506/02 ,  C12N2510/00 ,  C12N2533/90 ,  C12N2799/027

Abstract: The present invention provides methods to produce pluripotent stem cells from adult cells. In particular, the present invention provides methods to produce pluripotent stem cells from somatic cells without the use of a feeder-cell layer or an agent that increases efficiency of retroviral transfection.

Abstract translation: 本发明提供了从成体细胞产生多能干细胞的方法。 具体而言，本发明提供了从体细胞产生多能干细胞而不使用饲养细胞层或提高逆转录病毒转染效率的试剂的方法。

公开(公告)号：WO2011053763A3

公开(公告)日：2011-05-05

申请号：PCT/US2010/054662

申请日：2010-10-29

Applicant: CENTOCOR ORTHO BIOTECH INC. ,  NASO, Michael ,  SWEET, Raymond ,  LUO, Jinquan ,  WU, Sheng-jiun ,  ELLOSO, Merle ,  DUCATA, Daniela, Della ,  RAUCHENBERGER, Robert ,  RUTZ, Mark ,  ALMAGRO, Juan, Carlos ,  TAUDTE, Susann ,  WU, Bingyuan ,  OBMOLOVA, Galina ,  MALIA, Thomas

Inventor： NASO, Michael ,  SWEET, Raymond ,  LUO, Jinquan ,  WU, Sheng-jiun ,  ELLOSO, Merle ,  DUCATA, Daniela, Della ,  RAUCHENBERGER, Robert ,  RUTZ, Mark ,  ALMAGRO, Juan, Carlos ,  TAUDTE, Susann ,  WU, Bingyuan ,  OBMOLOVA, Galina ,  MALIA, Thomas

IPC: A61K39/395 ,  C07K16/00

Abstract: Interleukin-17A (IL-17A) antibody antagonists, polynucleotides encoding IL-17A antibody antagonists or fragments thereof, and methods of making and using the foregoing are disclosed.

公开(公告)号：WO2011011349A3

公开(公告)日：2011-05-05

申请号：PCT/US2010042504

申请日：2010-07-20

Applicant: CENTOCOR ORTHO BIOTECH INC ,  CLEVELAND CLINIC FOUNDATION ,  XU JEAN ,  JENSEN JAN

Inventor： XU JEAN ,  JENSEN JAN

IPC: C12N5/071 ,  C12N5/02 ,  C12N5/0735

CPC classification number: C12N5/0678 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2506/02 ,  C12N2533/90

Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells into cells expressing markers characteristic of the pancreatic endocrine lineage that co-express PDX1, NKX6.1, but do not express CDX2 and NGN3.

Abstract translation: 本发明提供了促进多能干细胞分化成表达具有共表达PDX1，NKX6.1但不表达CDX2和NGN3的胰腺内分泌谱系特征性标志物的细胞的方法。

公开(公告)号：WO2011047298A2

公开(公告)日：2011-04-21

申请号：PCT/US2010/052894

申请日：2010-10-15

Applicant: CENTOCOR ORTHO BIOTECH INC. ,  OLSON, Lorin, P. ,  KRULEVITCH, Peter ,  GLENCROSS, James ,  WANG, Jingli ,  FOLEY, Nicholas ,  ZHAO, Mingqi

Inventor： OLSON, Lorin, P. ,  KRULEVITCH, Peter ,  GLENCROSS, James ,  WANG, Jingli ,  FOLEY, Nicholas ,  ZHAO, Mingqi

IPC: A61M5/32 ,  A61M5/20

CPC classification number: A61M5/3202 ,  A61M5/20 ,  A61M5/2033 ,  A61M5/2046 ,  A61M5/2053 ,  A61M5/3129 ,  A61M5/315 ,  A61M5/31511 ,  A61M5/3157 ,  A61M5/3204 ,  A61M5/321 ,  A61M5/3243 ,  A61M5/326 ,  A61M5/3287 ,  A61M5/50 ,  A61M5/5086 ,  A61M2005/2026 ,  A61M2005/208 ,  A61M2005/3123 ,  A61M2005/3125 ,  A61M2005/3126 ,  A61M2005/3247 ,  A61M2205/581 ,  A61M2205/582 ,  A61M2205/583 ,  A61M2205/584 ,  A61M2205/586

Abstract: Disclosed is a device for the parenteral delivery of a medication, such as a drug. The device includes upper and lower housings in which the upper housing is configured to move relative to the lower housing as a result of application of an external force to permit the user of the device to control the rate at which the drug is administered.

Abstract translation: 公开了一种用于肠胃外递送药物如药物的装置。 该装置包括上部壳体和下部壳体，其中上部壳体被构造成由于施加外力而允许装置的使用者控制药物施用的速率而相对于下部壳体移动。 p>