公开(公告)号：WO2014165091A3

公开(公告)日：2014-11-27

申请号：PCT/US2014024346

申请日：2014-03-12

Applicant: CYTOVIVA INC

Inventor： BEACH JAMES M ,  LAWRENCE SAMUEL M ,  CHEATHAM BYRON J ,  UERTZ JAMES L ,  DOUGHERTY ROBERT P

IPC: G01N21/64 ,  B22F1/00 ,  B82Y35/00 ,  G01N15/00 ,  G02B21/10 ,  G02B21/36

CPC classification number: G06T17/00 ,  G01N21/6458 ,  G01N2015/0038 ,  G01N2015/1445 ,  G02B21/10 ,  G02B21/16 ,  G02B21/367 ,  G06T5/004 ,  G06T7/0012 ,  G06T15/08 ,  G06T15/506 ,  G06T2200/04 ,  G06T2207/10056 ,  G06T2207/30004 ,  G06T2210/41

Abstract: Disclosed are various embodiments for methods and systems for three-dimensional imaging of subject particles in media through use of dark-field microscopy. Some examples, among others, include a method for obtaining a three-dimensional (3D) volume image of a sample, a method for determining a 3D location of at least one subject particle within a sample, a method for determining at least one spatial correlation between a location of at least one subject particle and a location of at least one cell structure within a cell and/or other similar biological or nonbiological structure, a method of displaying a location of at least one subject particle, method for increasing the dynamic range of a 3D image acquired from samples containing weak and strong sources of light, and method for sharpening a 3D image in a vertical direction.

Abstract translation: 公开了用于通过使用暗视野显微镜对介质中的被摄体颗粒进行三维成像的方法和系统的各种实施例。 一些示例包括用于获得样本的三维（3D）体积图像的方法，用于确定样本内的至少一个对象粒子的3D位置的方法，用于确定至少一个空间相关性的方法 在至少一个主体颗粒的位置和细胞内和/或其他类似的生物或非生物结构之间的至少一个细胞结构的位置之间，显示至少一个主体颗粒的位置的方法，用于增加动态范围的方法 从包含弱光和强光源的样本获取的3D图像以及用于在垂直方向上锐化3D图像的方法。