公开(公告)号：WO2018114573A1

公开(公告)日：2018-06-28

申请号：PCT/EP2017/082776

申请日：2017-12-14

Applicant: DSM IP ASSETS B.V.

Inventor： KOUWEN, Roelof Hendrik Matthijs ,  VAN SINDEREN, Douwe ,  MCDONNELL, Brian ,  VER LOREN VAN THEMAAT, Pieter, Emiel ,  MAHONY, Jennifer

IPC: C12R1/46 ,  A23C9/123 ,  C12Q1/14

Abstract: The present invention relates to a starter culture comprising at least two Streptococcus thermophilus strains, wherein a first and a second Streptococcus thermophilus strain are chosen from RGP group 1, RGP group 2, RGP group 3 and RGP group 4, with the proviso that the first and second Streptococcus thermophilus strains do not belong to the same RGP group.

公开(公告)号：WO2016012552A1

公开(公告)日：2016-01-28

申请号：PCT/EP2015/066910

申请日：2015-07-23

Applicant: DSM IP ASSETS B.V.

Inventor： KOUWEN, Roelof Hendrik Matthijs ,  HANEMAAIJER, Laurens Leendert ,  VAN SINDEREN, Douwe ,  MCDONNELL, Brian ,  MAHONY, Jennifer

IPC: C12N1/20 ,  C12N1/36 ,  A23C19/032 ,  A23C9/123

CPC classification number: A23C9/1238 ,  A23C19/0323 ,  A23Y2240/75 ,  C12N1/20 ,  C12N1/36 ,  C12N15/113 ,  C12N15/746 ,  C12N15/902 ,  C12N2310/11

Abstract: The present invention provides a method for the construction of a bacteriophage- insensitive mutant of a Streptococcus thermophilus parent strain whereby the bacteriophage insensitivity is not based on the CRISPR resistance mechanism but based on another mechanism. The method provides a protocol by which this can be achieved by inactivating one or more of the CRISPR systems present in a given strain. The invention also provides the bacteriophage-insensitive mutants as well as their use in a process for making a dairy product.

Abstract translation: 本发明提供了构建嗜热链球菌亲本菌株的噬菌体不敏感突变体的方法，其中噬菌体不敏感性不是基于CRISPR抗性机制而是基于另一种机制。 该方法提供了通过使给定菌株中存在的一种或多种CRISPR系统失活来实现的方案。 本发明还提供了噬菌体不敏感突变体以及它们在制备乳制品的方法中的用途。

公开(公告)号：WO2015124718A1

公开(公告)日：2015-08-27

申请号：PCT/EP2015/053601

申请日：2015-02-20

Applicant: DSM IP ASSETS B.V.

Inventor： KOUWEN, Roelof Hendrik Matthijs ,  HEE, VAN, Pim ,  VAN SINDEREN, Douwe ,  MCDONNELL, Brian ,  MAHONY, Jennifer

IPC: C12N15/01 ,  C12N15/10 ,  C12Q1/68 ,  A23C9/123 ,  C12R1/46

CPC classification number: C12R1/46 ,  A23C9/1238 ,  A23C19/0323 ,  A23Y2240/75 ,  C12N1/04 ,  C12N1/20 ,  C12N15/01 ,  C12Q1/689 ,  G06F19/22

Abstract: Bacteriophage Insensitive Mutants (BIMs) of three Streptococcus thermophilus parent strains were generated and characterized for phage sensitivity, sedimentation rate, cell chain length, phage adsorption and CRISPR locialterations. Several BIMs showed an altered sedimentation phenotype as well as an increase cell chain length, reduced phage sensitivity, reduced phage adsorption and 100% identity in three CRISPR loci. The results show that the derived BIMs have become phage-resistant through a mechanism other than CRISPR.

Abstract translation: 产生三种嗜热链球菌亲本菌株的噬菌体不敏感突变体（BIM），其特征在于噬菌体敏感性，沉降速率，细胞链长度，噬菌体吸附和CRISPR定位。 几个BIM显示出改变的沉降表型以及增加的细胞链长度，降低的噬菌体敏感性，减少的噬菌体吸附和100个同一性在三个CRISPR基因座。 结果表明，衍生的BIMs通过CRISPR以外的机制已经成为抗噬菌体。