公开(公告)号：WO2004035806A2

公开(公告)日：2004-04-29

申请号：PCT/US2003/025044

申请日：2003-08-08

Applicant: EPIGENOMICS AG ,  COTTRELL, Susan ,  MOONEY, Suzanne

Inventor： COTTRELL, Susan ,  MOONEY, Suzanne

IPC: C12Q

CPC classification number: C07H21/04 ,  C12Q1/6827 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/154 ,  C12Q2563/167 ,  C12Q2531/113 ,  C12Q2523/125

Abstract: The disclosed invention provides methods and sequences for the analysis of methylation patterns within a novel 5' upstream CpG island of the calcitonin gene. Particular embodiments provide methylation-altered DNA sequences as novel diagnostic, prognostic and therapeutic markers for cancer.

Abstract translation: 所公开的发明提供了用于分析降钙素基因的新的5'上游CpG岛内的甲基化模式的方法和序列。 具体实施方案提供甲基化改变的DNA序列作为癌症的新型诊断，预后和治疗标记物。

公开(公告)号：WO2003066895A3

公开(公告)日：2003-08-14

申请号：PCT/EP2003/001218

申请日：2003-02-06

Applicant: EPIGENOMICS AG ,  COTTRELL, Susan

Inventor： COTTRELL, Susan

IPC: C12Q1/68

Abstract: Described is a method for methylation detection in a DNA sample. An isolated genomic DNA sample is treated in a manner capable of distinguishing methylated from unmethy-lated cytosine bases. The pretreated DNA is amplified us-ing at least one oligonucleotide primer, a polymerase and a set of nucleotides of which at least one is labeled with a first type of label. A sequence-specific oligonu-cleotide probe, marked with a second type of label, hy-bridizes to the amplification product and a FRET reaction occurs if a labeled oligonucleotide is present in close proximity in the amplification product. The method deter-mines the level of methylation of a sample by measuring the extent of fluorescence resonance energy transfer (FRET) between the donor and acceptor fluorophore.

公开(公告)号：WO2003066895A2

公开(公告)日：2003-08-14

申请号：PCT/EP2003/001218

申请日：2003-02-06

Applicant: EPIGENOMICS AG ,  COTTRELL, Susan

Inventor： COTTRELL, Susan

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/6818 ,  C12Q2545/114 ,  C12Q2535/113 ,  C12Q2531/107

Abstract: Described is a method for methylation detection in a DNA sample. An isolated genomic DNA sample is treated in a manner capable of distinguishing methylated from unmethy-lated cytosine bases. The pretreated DNA is amplified us-ing at least one oligonucleotide primer, a polymerase and a set of nucleotides of which at least one is labeled with a first type of label. A sequence-specific oligonu-cleotide probe, marked with a second type of label, hy-bridizes to the amplification product and a FRET reaction occurs if a labeled oligonucleotide is present in close proximity in the amplification product. The method deter-mines the level of methylation of a sample by measuring the extent of fluorescence resonance energy transfer (FRET) between the donor and acceptor fluorophore.

Abstract translation: 描述了DNA样品中甲基化检测的方法。 分离的基因组DNA样品以能够区分甲基化和未甲基化胞嘧啶碱基的方式进行处理。 使用至少一种寡核苷酸引物，聚合酶和一组核苷酸扩增预处理的DNA，其中至少一种核苷酸用第一种标记物标记。 用第二种标记标记的序列特异性寡核苷酸探针与扩增产物杂交，如果扩增产物中存在标记的寡核苷酸，则发生FRET反应。 该方法通过测量供体和受体荧光团之间的荧光共振能量转移（FRET）的程度来确定样品的甲基化水平。

公开(公告)号：WO2008011620A2

公开(公告)日：2008-01-24

申请号：PCT/US2007/074106

申请日：2007-07-23

Applicant: EPIGENOMICS AG ,  LOFTON-DAY, Catherine ,  SLEDZIEWSKI, Andrew ,  MODEL, Fabian ,  COTTRELL, Susan ,  DISTLER, Juergen ,  TETZNER, Reimo ,  DIETRICH, Dimo

Inventor： LOFTON-DAY, Catherine ,  SLEDZIEWSKI, Andrew ,  MODEL, Fabian ,  COTTRELL, Susan ,  DISTLER, Juergen ,  TETZNER, Reimo ,  DIETRICH, Dimo

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/154

Abstract: The invention provides methods, nucleic acids and kits for detecting colorectal cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of said class of disorders, thereby enabling the improved diagnosis and treatment of patients.

Abstract translation: 本发明提供用于检测结肠直肠细胞增殖性疾病的方法，核酸和试剂盒。 本发明公开的基因组序列，其中的甲基化模式对所述类病症的改进的检测工具，从而使得患者的改进的诊断和治疗。

公开(公告)号：WO2005054517A2

公开(公告)日：2005-06-16

申请号：PCT/US2004/040289

申请日：2004-12-01

Applicant: EPIGENOMICS AG ,  DAY, Kevin, J. ,  COTTRELL, Susan ,  DISTLER, Jüergen ,  MOROTTI, Andrew ,  YAMAMURA, Su ,  DEKKER, Sharon ,  OCAMP, Yreka ,  DEVOS, Theo

Inventor： DAY, Kevin, J. ,  COTTRELL, Susan ,  DISTLER, Jüergen ,  MOROTTI, Andrew ,  YAMAMURA, Su ,  DEKKER, Sharon ,  OCAMP, Yreka ,  DEVOS, Theo

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/154 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: The following application provides methods and nucleic acids for the detection of and/or differentiation between prostate cell proliferative disorders. This is achieved by the analysis of the expression status of a panel of genes, or subsets thereof.

Abstract translation: 以下应用提供了用于前列腺细胞增殖性疾病的检测和/或分化的方法和核酸。 这是通过分析一组基因或其子集的表达状态来实现的。

公开(公告)号：WO2003072820A2

公开(公告)日：2003-09-04

申请号：PCT/EP2003/002034

申请日：2003-02-27

Applicant: EPIGENOMICS AG ,  ADORJAN, Peter ,  BURGER, Matthias ,  MAIER, Sabine ,  LESCHE, Ralf ,  COTTRELL, Susan ,  MOONEY, Suzanne

Inventor： ADORJAN, Peter ,  BURGER, Matthias ,  MAIER, Sabine ,  LESCHE, Ralf ,  COTTRELL, Susan ,  MOONEY, Suzanne

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/154 ,  Y10T436/143333

Abstract: The invention provides methods and nucleic acids for detecting, differentiating or distinguishing between colon cell proliferative disorders by analysis of one or more of the genes Versican, TPEF, H-Cadherin, Calcitonin, and EYA4. The invention further provides novel nucleic acid sequences useful for the cell proliferative disorder specific analysis of said genes as well as methods, assays and kits thereof.

Abstract translation: 本发明提供了通过分析一种或多种基因Versican，TPEF，H-钙粘着蛋白，降钙素和EYA4来检测，分化或鉴别结肠细胞增殖性疾病的方法和核酸。 本发明还提供了用于所述基因的细胞增殖性病症特异性分析的新型核酸序列及其方法，测定和试剂盒。

公开(公告)号：WO2006071466A3

公开(公告)日：2006-07-06

申请号：PCT/US2005/043974

申请日：2005-12-02

Applicant: EPIGENOMICS AG ,  COTTRELL, Susan ,  MODEL, Fabian ,  HAEFLIGER, Carolina ,  WEISS, Gunter ,  DISTLER, Jürgen ,  SLEDZIEWSKI, Andrew, Z. ,  SONG, Xiaoling ,  SKILLMAN, Thomas, L. ,  THOMAS, Jeffrey, G.

Inventor： COTTRELL, Susan ,  MODEL, Fabian ,  HAEFLIGER, Carolina ,  WEISS, Gunter ,  DISTLER, Jürgen ,  SLEDZIEWSKI, Andrew, Z. ,  SONG, Xiaoling ,  SKILLMAN, Thomas, L. ,  THOMAS, Jeffrey, G.

IPC: C12Q1/68

Abstract: Particular aspects provide novel methods and compositions (e.g., nucleic acids, kits, etc.) having substantial utility for providing a prognosis of prostate cell proliferative disorders. In particular aspects, this is achieved by the analysis of the expression status of a panel of genes, or subsets thereof.

公开(公告)号：WO2004067777A1

公开(公告)日：2004-08-12

申请号：PCT/US2004/001987

申请日：2004-01-23

Applicant: EPIGENOMICS AG ,  COTTRELL, Susan ,  SLEDZIEWSKI, Andrew

Inventor： COTTRELL, Susan ,  SLEDZIEWSKI, Andrew

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/154 ,  C12Q2600/156

Abstract: The present invention provides for molecular GSTP1 markers that have novel utility for the analysis of methylation patterns within the promoter region and exons 1 and 2 of the GSTP1 gene, and are further useful in methods to effectively distinguish among benign hyperplasia of the prostate and different grades of prostate cancer. Additionally, the subject molecular GSTP1 markers have novel utility for the precise localization of the zone of origin to provide sensitive, accurate and non-invasive methods for the diagnosis and/or prognosis of prostate cell proliferative disorders. The present invention has novel utility for the detection and differentiation of a cell proliferative disorder of the peripheral zone of the prostate.

Abstract translation: 本发明提供了分子GSTP1标记，其具有用于分析启动子区域和GSTP1基因的外显子1和2之间的甲基化模式的新型用途，并且还可用于有效区分前列腺的良性增生和不同等级的方法 的前列腺癌。 另外，本发明的分子GSTP1标记物具有用于准确定位起始区域以提供用于前列腺细胞增殖性疾病的诊断和/或预后的灵敏，准确和非侵入性方法的新颖效用。 本发明具有用于检测和分化前列腺周边区域的细胞增殖性疾病的新用途。

公开(公告)号：WO2003072820A3

公开(公告)日：2003-09-04

申请号：PCT/EP2003/002034

申请日：2003-02-27

Applicant: EPIGENOMICS AG ,  ADORJAN, Peter ,  BURGER, Matthias ,  MAIER, Sabine ,  LESCHE, Ralf ,  COTTRELL, Susan ,  MOONEY, Suzanne

Inventor： ADORJAN, Peter ,  BURGER, Matthias ,  MAIER, Sabine ,  LESCHE, Ralf ,  COTTRELL, Susan ,  MOONEY, Suzanne

IPC: C12Q1/68

Abstract: The invention provides methods and nucleic acids for detecting, differentiating or distinguishing between colon cell proliferative disorders by analysis of one or more of the genes Versican, TPEF, H-Cadherin, Calcitonin, and EYA4. The invention further provides novel nucleic acid sequences useful for the cell proliferative disorder specific analysis of said genes as well as methods, assays and kits thereof.