公开(公告)号：WO1989004302A1

公开(公告)日：1989-05-18

申请号：PCT/US1988003947

申请日：1988-11-04

Applicant: IGEN, INC.

Inventor： IGEN, INC. ,  MASSEY, Richard, J. ,  POWELL, Michael, J. ,  DRESSICK, Walter, J. ,  LELAND, Jonathan, K. ,  HINO, Janel, K. ,  POONIAN, Mohindar, S. ,  DELLA CIANA, Leopoldo

IPC: C07D213/89

CPC classification number: C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F13/005 ,  C07H21/00 ,  C12Q1/6816 ,  G01N33/533 ,  G01N33/582 ,  G01N2458/30 ,  C12Q2563/103

Abstract: Electrochemiluminescent moieties having the formula [Re(P)m(L )n(L )o(L )p(L )q(L )r(L )s]t(B)u, wherein P is a polydentate ligand of Re; L , L , L , L , L and L are ligands of Re, each of which may be the same as or different from each other ligand; B is a substance which is a ligand of Re or is conjugated to one or more of p, L , L , L , L , L or L ; m is an integer equal to or greater than 1; each of n, o, p, q, r and s is zero or an integer; t is an integer equal to or greater than 1; and u is an integer equal to or greater than 1; p, L , L , L , L , L , L and B being of such composition and number that the chemical moiety can be induced to emit electromagnetic radiation and the total number of bonds to Re provided by the ligands of Re being equal to the coordination of Re are disclosed. Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids using these moieties are disclosed. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety containing rhenium and capable of combining with the analyte of interest, exposing the resulting sample to chemical, electrochemical, or electromagnetic energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.

Abstract translation: 具有式[Re（P）m（L 1）n（L 2）o（L 3）p（L 4）q（L 5）r（L 6）的电化学发光部分 >）s] t（B）u，其中P是Re的多齿配体; L 1，L 2，L 3，L 4，L 5和L 6是Re的配体，其各自可以彼此相同或不同; B是作为Re配体的物质，或与p，L 1，L 2，L 3，L 4，L 5或L 6中的一个或多个相结合的物质。 m是等于或大于1的整数; n，o，p，q，r和s中的每一个为零或整数; t是等于或大于1的整数; u是等于或大于1的整数; P，L 1，L 2，L 3，L 4，L 5，L 6和B具有这样的组成和化学部分可以诱导发射电磁辐射的数量 并且公开了由Re配体提供的Re的总数等于Re的配位。 公开了使用这些部分存在于多组分液体中的感兴趣分析物的定性和定量的电化学发光测定。 这些方法包括使样品与含有铼的电化学发光化学部分标记的试剂接触并能够与感兴趣的分析物结合，将所得样品暴露于化学，电化学或电磁能并检测由电化学发光化学部分发射的电磁辐射。

公开(公告)号：WO1996005501A1

公开(公告)日：1996-02-22

申请号：PCT/US1994009124

申请日：1994-08-15

Applicant: IGEN, INC.

Inventor： IGEN, INC. ,  GHAED, Ali ,  LELAND, Jonathan, K. ,  ZOSKI, Glenn, D. ,  GOODMAN, Jack, E. ,  GROSSER, John, T.

IPC: G01N21/76

CPC classification number: G01N35/025 ,  G01N21/76 ,  G01N2035/00356 ,  G01N2035/0486

Abstract: An apparatus for detecting the presence of analytes of interest by inducing and detecting electrocheluminescence in a test sample includes an agitated carousel (140) to releasably support a plurality of sample tubes (142).

Abstract translation: 用于通过诱导和检测测试样品中的电致发光来检测感兴趣分析物的存在的装置包括可释放地支撑多个样品管（142）的搅拌转盘（140）。

公开(公告)号：WO1996041845A1

公开(公告)日：1996-12-27

申请号：PCT/US1996009917

申请日：1996-06-07

Applicant: IGEN, INC.

Inventor： IGEN, INC. ,  JAMEISON, Fabian ,  LELAND, Jonathan, K.

IPC: C09K03/00

CPC classification number: C12Q1/60 ,  C01B15/01 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/54 ,  G01N21/76 ,  G01N33/582 ,  Y10S436/805

Abstract: The invention is directed to processes and compositions useful for detecting the presence and/or the amount of reagents including H2O2, H2O2-producing components, or H2O2-consuming components via modulation effects that these reagents have upon the oxidative-reduction electrochemiluminescent reaction mechanism among the oxalate anion (C2O4 ) and ruthenium (II)tris(2, 2'-bipyridine).

Abstract translation: 本发明涉及用于通过这些试剂对氧化还原电化学发光反应机理的调节作用来检测包括H 2 O 2，H 2 O 2产生组分或H2O2消耗组分的试剂的存在和/或量的方法和组合物 草酸盐阴离子（C2O4 <＆amp; H2>）和钌（Ⅱ）三（2,2'-联吡啶）。

公开(公告)号：WO1996015440A1

公开(公告)日：1996-05-23

申请号：PCT/US1995014847

申请日：1995-11-13

Applicant: IGEN, INC.

Inventor： IGEN, INC. ,  TALLEY, David, B. ,  LELAND, Jonathan, K.

IPC: G01N21/76

CPC classification number: G01N21/76 ,  G01N21/69 ,  G01N33/54326 ,  G01N33/582 ,  G01N35/0098

Abstract: A method and apparatus for measuring electrochemiluminescence from a sample composition are described wherein magnetically responsive electrochemiluminescent active species are captured on the electrode (5) with the aid of a capture magnet (15) having a configuration such that the magnetic flux lines (or the magnetic field gradient) of at least one magnetic field source therein are compressed and/or dispersed. This capture magnet (15) improves the distribution of the magnetically responsive electrochemiluminescent active species on the electrode surface (5) and reduces interference with the photomultiplier tube (20), thereby enhancing the ECL signal and improving sensitivity. The improved capture and distribution also allows for shorter assay times.

Abstract translation: 描述了一种用于从样品组合物测量电化学发光的方法和装置，其中磁响应电化学发光活性物质借助捕获磁体（15）捕获在电极（5）上，捕获磁体（15）具有使得磁通线（或磁 其中至少一个磁场源的场梯度）被压缩和/或分散。 这种捕获磁体（15）改善了电响应性电化学发光活性物质在电极表面（5）上的分布，并减少了对光电倍增管（20）的干扰，从而增强了ECL信号并提高了灵敏度。 改进的捕获和分布也允许更短的测定时间。

公开(公告)号：WO1996041177A1

公开(公告)日：1996-12-19

申请号：PCT/US1996009870

申请日：1996-06-06

Applicant: IGEN, INC.

Inventor： IGEN, INC. ,  BILLADEAU, Mark, A. ,  LELAND, Jonathan, K. ,  SHEN, Lihong ,  GUDIBANDE, Satyanarayana, R.

IPC: G01N33/536

CPC classification number: G01N33/582 ,  C09K11/07 ,  G01N33/533

Abstract: An electrochemiluminescent (ECL) detection method for simultaneous assays. The method employs an excitation process whereby one or more luminophores are excited through an antenna mechanism using an oxidative or reductive coreactant. The luminophores can be lanthanide chelates and a persulfate system is used to generate ECL. The ECL emissions may be separated either by measuring at different emission wavelengths or by electrode potentials.

Abstract translation: 用于同时测定的电化学发光（ECL）检测方法。 该方法采用激发过程，其中一个或多个发光体通过使用氧化还原共反应物的天线机构激发。 发光体可以是镧系元素螯合物，并且使用过硫酸盐体系来产生ECL。 ECL排放可以通过在不同的发射波长或电极电位进行测量来分离。

公开(公告)号：WO1996041155A1

公开(公告)日：1996-12-19

申请号：PCT/US1996010167

申请日：1996-06-06

Applicant: IGEN, INC.

Inventor： IGEN, INC. ,  LELAND, Jonathan, K. ,  TALLEY, David, B. ,  HAYES, Stephanie, A.

IPC: G01N21/66

CPC classification number: G01N21/69 ,  G01N21/76

Abstract: The invention is a method for conducting an electrochemiluminescent assay that includes contacting a sample containing an analyte of interest with a first electrical waveform, followed by contacting the sample with a second electrical waveform that has been modulated in an amount derived from a pre-determined gain extension factor.

Abstract translation: 本发明是用于进行电化学发光测定的方法，其包括将含有目标分析物的样品与第一电波形接触，然后使样品与已经以预定增益得到的量调制的第二电波形接触 延伸因子。

公开(公告)号：WO1996036870A1

公开(公告)日：1996-11-21

申请号：PCT/US1996006948

申请日：1996-05-16

Applicant: IGEN, INC.

Inventor： IGEN, INC. ,  TALLEY, David ,  LELAND, Jonathan, K. ,  BLACKBURN, Gary, F.

IPC: G01N27/26

CPC classification number: C12Q1/001 ,  G01N33/5438 ,  Y10S435/817

Abstract: An electrode can be derivitized by contacting it with a derivitizing solution to make it more sensitive to a desired analyte signal as opposed to interfering signals in an assay. Particularly, in an electrochemiluminescence (ECL) immunoassay the working electrode can be derivitized to be more sensitive to desired analyte signals, as opposed to interfering non-bound conjugate or serum matrix signals.

Abstract translation: 通过使其与衍生溶液接触可以衍生出电极，使其对所需分析物信号的敏感性与测定中的干扰信号相反。 特别地，在电化学发光（ECL）免疫测定中，与干扰非结合的缀合物或血清基质信号相反，工作电极可被衍生为对所需分析物信号更敏感。

公开(公告)号：WO1996024690A1

公开(公告)日：1996-08-15

申请号：PCT/US1996001113

申请日：1996-02-09

Applicant: IGEN, INC.

Inventor： IGEN, INC. ,  BARD, Allen, J. ,  RICHARDS, Thomas, C. ,  LELAND, Jonathan, K.

IPC: C12Q01/68

CPC classification number: C12Q1/005 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N33/582

Abstract: Biomolecule analysis using anodic oxidation of aqueous sodium 9, 10-diphenylanthracene-2-sulfonate (DPAS) and 1- and 2-thianthrenecarboxylic acid (1-THCOOH and 2-THCOOH) in the presence of tri-n- propylamine (TPrA) as a coreactant in aqueous solution produces electrogenerated chemiluminescence (ECL). In addition, the cathodic reduction of DPAS in the presence of peroxydisulfate (S2O8 ) as a coreactant also produces ECL in an acetonitrile (MeCN)-water solution (1:1 by volume). The oxidation of chlorpromazine (CPZ) produces an ECL emission in the absence of an added coreactant following an unprecedented "self-annihilation" mechanism.

Abstract translation: 在三正丙胺（TPrA）存在下，使用9,10-二苯基蒽-2-磺酸钠（DPAS）和1-和2-噻吩甲酸（1-THCOOH和2-THCOOH）的水溶液阳极氧化的生物分子分析 水溶液中的共反应物产生电致化学发光（ECL）。 此外，作为共反应物的过氧化二硫酸盐（S2O8 2-）存在下DPAS的阴极还原还在乙腈（MeCN） - 水溶液（体积比1:1）中产生ECL。 氯丙嗪（CPZ）的氧化在没有加入的共反应物的情况下，在空前的“自我消灭”机制之后产生ECL排放。

公开(公告)号：WO1996017248A1

公开(公告)日：1996-06-06

申请号：PCT/US1995015982

申请日：1995-12-04

Applicant: IGEN, INC.

Inventor： IGEN, INC. ,  NACAMULLI, Laurette ,  LELAND, Jonathan, K. ,  HAYES, Stephanie, A.

IPC: G01N33/557

CPC classification number: C12Q1/00 ,  G01N33/557 ,  G01N33/582

Abstract: A biomolecular reaction, such as an enzymatic or affinity binding reaction is effected in an electrochemical cell with a mixture of reagents including a luminophore which will relate the concentration of a reactant, a reaction partner or the reaction product of a reaction partner to the electrochemiluminescent intensity, in order to determine the rate of the biomolecular reaction. The reaction partner is a reagent which reacts with the reactant and which itself or its reaction product participates with the luminophore to cause ECL emmissions. The ECL intensity is modulated with a series of electrical pulses which are applied to the mixture of reagents at a preselected potential for preselected intervals of time and duration. The intensity is measured during those intervals to provide a timed series of values (P). The same experiment is repeated twice more, once wherein the modulation is conducted after the reaction has gone to completion to obtain the timed series of values (C), and the last time the reaction is performed in the absence of the reaction partner to obtain the timed series of values (B). The results are normalized (N) using the formula I to obtain a series of values (N) which are plotted to obtain the time course of reaction.

Abstract translation: 生物分子反应如酶或亲和力结合反应在电化学电池中进行，其中包括发光体的试剂混合物，其将反应物，反应配对物或反应产物与电化学发光强度相关联 ，以确定生物分子反应的速率。 反应伴侣是与反应物反应的试剂，其本身或其反应产物与发光体共同引发ECL排放。 ECL强度用一系列电脉冲进行调制，这些电脉冲以预先选定的时间和持续时间间隔施加到试剂的混合物。 在这些间隔期间测量强度以提供定时的一系列值（P）。 重复两次相同的实验，一次在反应完成后进行调制以获得时间序列值（C），最后一次在没有反应配偶体的情况下进行反应以获得 定时系列值（B）。 使用公式I对结果进行标准化（N）以获得一系列值（N），其绘制以获得反应的时间过程。

公开(公告)号：WO1993001308A1

公开(公告)日：1993-01-21

申请号：PCT/US1992005788

申请日：1992-07-10

Applicant: IGEN, INC.

Inventor： IGEN, INC. ,  MASSEY, Richard, J. ,  BLACKBURN, Gary, F. ,  WILKINS, Elizabeth, W. ,  SHAH, Haresh, P. ,  LELAND, Jonathan, K.

IPC: C12Q01/28

CPC classification number: G01N33/54313 ,  G01N21/69 ,  G01N21/76 ,  G01N33/54326 ,  G01N33/5438 ,  G01N33/582 ,  G01N33/585 ,  G01N2458/30

Abstract: What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing the sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of particles capable of specifically binding with the analyte and/or the assay-performance-substance; incubating the composition to form a complex which includes a particle labeled component; collecting the complex in a collection zone; introducing into the collection zone a trigger capable of triggering the label such that the label luminesces; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.

Abstract translation: 描述了用于对样品中存在的感兴趣分析物进行结合测定的方法和装置。 所述方法包括以下步骤：形成含有样品的组合物，包含与触发时能够化学发光的标记化合物连接的组分的测定性能物质，以及能够与分析物和/或 测定性能物质; 孵育组合物以形成包含颗粒标记的组分的复合物; 在收集区收集复合物; 向收集区引入能够触发标签以使标签发光的触发器; 并测量发射的发光以测量样品中感兴趣的分析物的存在。