公开(公告)号：WO1998007671A1

公开(公告)日：1998-02-26

申请号：PCT/US1996013746

申请日：1996-08-22

Applicant: IGEN, INC.

Inventor： IGEN, INC. ,  MOSBACH, Klaus ,  MARTIN, Mark, T. ,  MASSEY, Richard, J.

IPC: C07B55/00

CPC classification number: C07C51/47 ,  C07B57/00 ,  G01N2600/00 ,  C07C59/84 ,  C07C59/64 ,  C07C57/30

Abstract: The present invention is directed to the separation of optically active compounds into their respective enantiomers. The separation uses imprinted polymers having a desired structure and molecular memory for the corresponding print molecules. The invention is particularly concerned with the separation of ibuprofen, naproxen, and ketoprofen. Associated processes and materials such as certain polymers are also within the scope of the present invention.

Abstract translation: 本发明涉及将光学活性化合物分离成它们各自的对映异构体。 分离使用对于相应的印刷分子具有期望结构和分子记忆的印迹聚合物。 本发明特别涉及布洛芬，萘普生和酮洛芬的分离。 诸如某些聚合物的相关工艺和材料也在本发明的范围内。

公开(公告)号：WO1997038015A1

公开(公告)日：1997-10-16

申请号：PCT/US1997005151

申请日：1997-04-04

Applicant: IGEN, INC. ,  MOSBACH, Klaus ,  RAMSTRÖM, Olof

Inventor： IGEN, INC.

IPC: C07K16/44

CPC classification number: B01J20/261 ,  A61K38/00 ,  B01J20/26 ,  B01J20/268 ,  B01J20/3057 ,  C07K16/26 ,  G01N33/531 ,  G01N33/743

Abstract: The invention relates to artificial antibodies that are prepared by molecular imprinting, where methacrylic acid, ethylene glycol dimethacrylate and a print molecule are combined to form an artificial antibody having spatially positioned binding sites dictated by the corticosteroid print molecule, and the antibodies can be used in separation and analytical procedures.

Abstract translation: 本发明涉及通过分子印迹制备的人造抗体，其中将甲基丙烯酸，乙二醇二甲基丙烯酸酯和印刷分子组合以形成具有由皮质类固醇印刷分子指定的具有空间位置的结合位点的人造抗体，并且所述抗体可用于 分离和分析程序。

公开(公告)号：WO1996033411A1

公开(公告)日：1996-10-24

申请号：PCT/US1996005392

申请日：1996-04-18

Applicant: IGEN, INC.

Inventor： IGEN, INC. ,  YANG, Hongjun ,  CAIRNS, Nicholas

IPC: G01N33/53

CPC classification number: C07F5/003 ,  G01N33/582 ,  Y10S436/806 ,  Y10T436/13

Abstract: Luminescent chemical reagents that include complexes of rare earth metals with ligands such as aromatic heterocyclic nitrogen-containing compounds and semi-aromatic oxygen-containing compounds are used to detect small quantities of complex substances such as pharmaceuticals, metabolites, and microorganisms in complex sample mixtures.

Abstract translation: 包括稀土金属络合物与芳族杂环含氮化合物和半芳族含氧化合物等配体的发光化学试剂用于检测复杂样品混合物中的少量复合物质，如药物，代谢物和微生物。

公开(公告)号：WO1996006946A1

公开(公告)日：1996-03-07

申请号：PCT/US1995010630

申请日：1995-08-25

Applicant: IGEN, INC.

Inventor： IGEN, INC. ,  BARD, Allen, J. ,  XU, Xiao-Hong

IPC: C12Q01/00

CPC classification number: G01N33/582 ,  C12Q1/005 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6869

Abstract: Single-strand DNA was immobilized on an electrode covered with an aluminum alkanebisphosphonate film by immersing it in an ss-DNA solution. The immobilized ss-DNA labeled with Ru(bpy)3 was detected by monitoring the electrogenerated chemiluminescence (ECL) produced upon oxidation in a solution containing tri-n-propylamine. After immobilization of unlabeled ss-DNA, a complementary labeled strand DNA was hybridized to produce ds-DNA on the surface. The extent of DNA hybridization was determined by ECL of the labeled Ru(bpy)3 . Surface immobilized ds-DNA could also be detected by observing the ECL of intercalated Ru(phen)3 . Transmission electron microscopy (TEM) was employed to image the film and the immobilized DNA.

Abstract translation: 将单链DNA通过将其浸入ss-DNA溶液中而固定在覆盖有烷烃二膦酸铝膜的电极上。 通过监测在含有三正丙胺的溶液中氧化产生的电致化学发光（ECL）来检测用Ru（bpy）3 2+标记的固定的ss-DNA。 固定未标记的ss-DNA后，互补标记的链DNA被杂交以在表面上产生ds-DNA。 通过标记Ru（bpy）3 2+的ECL测定DNA杂交的程度。 表面固定的ds-DNA也可以通过观察插入的Ru（phen）3 2+的ECL来检测。 使用透射电子显微镜（TEM）对膜和固定化DNA进行成像。

公开(公告)号：WO1994025573A1

公开(公告)日：1994-11-10

申请号：PCT/US1994004437

申请日：1994-04-22

Applicant: IGEN, INC. ,  NAPPER, Andrew, D. ,  TITMAS, Richard, C. ,  MARTIN, Mark, T. ,  HONG, Wonpyo

Inventor： IGEN, INC.

IPC: C12N09/00

CPC classification number: C12N9/0002 ,  A61K38/00 ,  C07F9/3241 ,  C07F9/4816 ,  C07F9/5537 ,  C07K7/086 ,  C07K14/585 ,  C07K14/595

Abstract: Described and claimed are compounds of formula (I) wherein Y is a polypeptide, R1 is bonded to the N-terminus of Y and is hydrogen or a branched or linear, substituted or unsubstituted, C1-21 alkyl, alkene, or alkyne group, R2 is a side chain of a naturally occurring amino acid, and X is (a), (b), (c). Such compounds are useful as haptens and immunogens for the elicitation of antibodies which catalytically enhance the rate of formation or hydrolysis of primary amide bonds. Also described and claimed are methods employing the compounds and antibodies.

Abstract translation: 描述和要求保护的是式（I）的化合物，其中Y是多肽，R 1与Y的N末端键合，并且是氢或支链或直链，取代或未取代的C 1-11烷基，烯烃或炔基， R2是天然氨基酸的侧链，X是（a），（b），（c）。 这样的化合物可用作引发抗体的半抗原和免疫原，所述抗体催化增强初级酰胺键的形成或水解速率。 还描述和要求保护的是使用化合物和抗体的方法。

公开(公告)号：WO1993012245A1

公开(公告)日：1993-06-24

申请号：PCT/US1992010524

申请日：1992-12-07

Applicant: IGEN, INC.

Inventor： IGEN, INC. ,  KENTEN, John, H. ,  LINK, John, R.

IPC: C12P19/34

CPC classification number: C12Q1/708 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q1/689 ,  C12Q2531/107

Abstract: A process for exponentially amplifying a selected nucleic acid sequence present in a sample, comprising the steps of forming a mixture of the sample and a single primer designed to hybridize with the selected nucleic acid sequence; causing the single primer to hybridize to a single strand of the nucleic acid sequence of interest; forming a duplex product of the nucleic acid by a polymerase reaction; separating the duplex product into single strands; and repeating the preceding steps until the rate of production of the amplification product is exponential and the nucleic acid sequence of interest has been amplified.

Abstract translation: 一种用于对存在于样品中的所选择的核酸序列进行指数扩增的方法，包括以下步骤：形成所述样品和设计为与所选择的核酸序列杂交的单个引物的混合物; 使单个引物与感兴趣的核酸序列的单链杂交; 通过聚合酶反应形成核酸的双链体产物; 将双链体分离成单股; 并重复前述步骤，直到扩增产物的产生速率为指数，并且目的核酸序列已被扩增。

公开(公告)号：WO1993002703A1

公开(公告)日：1993-02-18

申请号：PCT/US1992006530

申请日：1992-08-04

Applicant: IGEN, INC.

Inventor： IGEN, INC. ,  KENTEN, John, Henry ,  VON BORSTEL, Reid ,  CASADEI, Jan, M. ,  KAMIREDDY, Balreddy ,  MARTIN, Mark, T. ,  MASSEY, Richard, J. ,  NAPPER, Andrew, D. ,  SIMPSON, David, M. ,  SMITH, Rodger, G. ,  TITMAS, Richard, C. ,  WILLIAMS, Richard, O.

IPC: A61K39/00

CPC classification number: G01N33/5011 ,  C07C311/19 ,  C07F9/2408 ,  C07F9/2458 ,  C07F9/4006 ,  C07F9/4075 ,  C07F9/650952 ,  C07F9/657181 ,  C07H15/252 ,  C07H19/04 ,  C07K16/468 ,  G01N33/5008 ,  G01N33/5014 ,  G01N33/5088 ,  G01N33/5094 ,  G01N2500/00

Abstract: Disclosed and claimed are prodrugs activated by catalytic proteins, such as enzymes and catalytic antibodies. The invention comprehends such prodrugs, as well as haptens, to elicit catalytic antibodies to activate the prodrugs. The prodrugs are useful as cytotoxic chemotherapeutic agents, e.g., as antitumor agents.

Abstract translation: 披露和要求的是由催化蛋白如酶和催化抗体活化的前药。 本发明包括这些前药以及半抗原以引发催化抗体以活化前药。 前药可用作细胞毒性化学治疗剂，例如作为抗肿瘤剂。

公开(公告)号：WO1990013661A1

公开(公告)日：1990-11-15

申请号：PCT/US1990002274

申请日：1990-04-25

Applicant: IGEN, INC. ,  SUDHIR, Paul

Inventor： IGEN, INC.

IPC: C12N09/48

CPC classification number: C07K14/57563 ,  C07K16/06 ,  C07K16/26 ,  C07K2317/34 ,  C12N9/0002 ,  C12N9/48

Abstract: Autoantibodies which enhance the rate of a chemical reaction of a substrate, processes for their preparation, their use and compositions thereof are disclosed. In particular, an autoantibody capable of catalyzing the hydrolysis of the peptide bond between amino acid residues Thr?7-Asp8, Arg14-Lys15, Gln16-Met17, Met17-Ala18, Ala18-Val19¿, Lys¿20?-Lys?21, Lys21-Tyr22¿ in the neurotransmitter vasoactive intestinal peptide (VIP) is disclosed.

公开(公告)号：WO1989010961A1

公开(公告)日：1989-11-16

申请号：PCT/US1989001951

申请日：1989-05-04

Applicant: IGEN, INC. ,  TITMAS, Richard, C. ,  HANSEN, David, E. ,  HONG, Wonpyo ,  BOOTH, Paul, M. ,  POWELL, Michael, J. ,  REES, Anthony, R. ,  MASSEY, Richard, J.

Inventor： IGEN, INC.

IPC: C12N09/14

CPC classification number: C07D207/404 ,  C07C229/46 ,  C07C229/48 ,  C07C235/74 ,  C07C271/24 ,  C07C311/06 ,  C07C317/44 ,  C07C2601/04 ,  C07C2602/42 ,  C07D205/085 ,  C07F9/2458 ,  C07F9/301 ,  C07F9/3211 ,  C07F9/4006 ,  C07F9/5537 ,  C07K16/00 ,  C07K16/1063 ,  C12N9/0002 ,  C12Q1/25 ,  G01N33/531 ,  G01N33/577 ,  G01N2333/16 ,  G01N2333/96472

Abstract: Antigens capable of eliciting antibodies which can catalyze chemical reactions, in particular, the cleavage or formation of a peptide linkage, comprising a hapten or a hapten and a suitable carrier molecule are disclosed. Antibodies which are catalytically active for chemical reactions, in particular, the cleavage or formation of a selected amide, ester or glycosidic bond, and which are elicited by such antigenes are disclosed as well as methods for producing the antibodies and methods for catalyzing the cleavage or formation of an amide, ester or glycosidic bond in a molecule.

Abstract translation: 公开了能够引发抗体的抗原，其可以催化化学反应，特别是包含半抗原或半抗原和合适的载体分子的肽键的切割或形成。 公开了用于化学反应的催化活性的抗体，特别是所选择的酰胺，酯或糖苷键的切割或形成，并且由这些抗原引发的抗体以及用于产生抗体的方法和用于催化切割的方法 在分子中形成酰胺，酯或糖苷键。

公开(公告)号：WO1989004302A1

公开(公告)日：1989-05-18

申请号：PCT/US1988003947

申请日：1988-11-04

Applicant: IGEN, INC.

Inventor： IGEN, INC. ,  MASSEY, Richard, J. ,  POWELL, Michael, J. ,  DRESSICK, Walter, J. ,  LELAND, Jonathan, K. ,  HINO, Janel, K. ,  POONIAN, Mohindar, S. ,  DELLA CIANA, Leopoldo

IPC: C07D213/89

CPC classification number: C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F13/005 ,  C07H21/00 ,  C12Q1/6816 ,  G01N33/533 ,  G01N33/582 ,  G01N2458/30 ,  C12Q2563/103

Abstract: Electrochemiluminescent moieties having the formula [Re(P)m(L )n(L )o(L )p(L )q(L )r(L )s]t(B)u, wherein P is a polydentate ligand of Re; L , L , L , L , L and L are ligands of Re, each of which may be the same as or different from each other ligand; B is a substance which is a ligand of Re or is conjugated to one or more of p, L , L , L , L , L or L ; m is an integer equal to or greater than 1; each of n, o, p, q, r and s is zero or an integer; t is an integer equal to or greater than 1; and u is an integer equal to or greater than 1; p, L , L , L , L , L , L and B being of such composition and number that the chemical moiety can be induced to emit electromagnetic radiation and the total number of bonds to Re provided by the ligands of Re being equal to the coordination of Re are disclosed. Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids using these moieties are disclosed. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety containing rhenium and capable of combining with the analyte of interest, exposing the resulting sample to chemical, electrochemical, or electromagnetic energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.

Abstract translation: 具有式[Re（P）m（L 1）n（L 2）o（L 3）p（L 4）q（L 5）r（L 6）的电化学发光部分 >）s] t（B）u，其中P是Re的多齿配体; L 1，L 2，L 3，L 4，L 5和L 6是Re的配体，其各自可以彼此相同或不同; B是作为Re配体的物质，或与p，L 1，L 2，L 3，L 4，L 5或L 6中的一个或多个相结合的物质。 m是等于或大于1的整数; n，o，p，q，r和s中的每一个为零或整数; t是等于或大于1的整数; u是等于或大于1的整数; P，L 1，L 2，L 3，L 4，L 5，L 6和B具有这样的组成和化学部分可以诱导发射电磁辐射的数量 并且公开了由Re配体提供的Re的总数等于Re的配位。 公开了使用这些部分存在于多组分液体中的感兴趣分析物的定性和定量的电化学发光测定。 这些方法包括使样品与含有铼的电化学发光化学部分标记的试剂接触并能够与感兴趣的分析物结合，将所得样品暴露于化学，电化学或电磁能并检测由电化学发光化学部分发射的电磁辐射。