摘要:
Assays (100) may be performed with a luminometer (400) having a chassis (405) that may include a reaction vessel chamber (610). The luminometer (400) may also include a light passage (640) that intersects the reaction vessel chamber (610). The luminometer (400) may also include a cap (415) that, when in a closed configuration, prevents light emitted by external sources from entering the reaction vessel chamber (610) and from entering the light passage (640). The cap (415) may provide access to the reaction vessel chamber (610) when in an open configuration. The luminometer (400) may also include a calibration light source (460) optically coupled to one end of the light passage (640) and a light detector (630) optically coupled to another end of the light passage (640). The light detector (630) may include a sensing element for receiving light from the light passage (640).
摘要:
A luminometer (400) includes a light detector (630) configured to sense photons (135). The luminometer (400) includes an analog circuit (915a) configured to provide an analog signal (965) based on the photons (135) emitted from assay reactions over a time period and a counter circuit (915b) configured to provide a photon count (970) based on the photons (135) emitted from the assay reactions over the time period. The luminometer (400) includes a luminometer controller (905) configured to, in response to an analog signal value of the analog signal (965) being greater than a predetermined value, determine and report a measurement value of the photons (135) emitted from the assay reactions over the time period based on the analog signal value of the analog signal (965) and a linear function (1010). Optionally, the linear function (1010) is derived from a relationship between the analog signal (965) and the photon count (970).
摘要:
Die vorliegende Erfindung betrifft eine Messvorrichtung (1) zum Messen von Lichtsignalen, welche von Proben in Wells (9) einer Mikroplatte (8) ausgesendet werden, insbesondere eine Lumineszenz-Messvorrichtung zum Messen von Lumineszenz-Signalen. In dieser Ausführungsform umfasst die Lumineszenz-Messvorrichtung (1) einen ersten Injektor (2) mit einer Injektornadel (6) und einem Auslassende (25) zum Abgeben eines Reagens in ein Well (9) sowie eine Lumineszenz-Optik (10) mit einer im Wesentlichen senkrecht zur Mikroplatte (8) verlaufenden optischen Achse (20) und einen die optische Achse (20) umschliessenden Messbereich (23). Die Injektornadel (6) erstreckt sich unter die Lumineszenz-Optik (10) und mündet mit ihrem Auslassende (25) ein deren Messbereich. Die Lumineszenz-Messvorrichtung (1) umfasst zudem einen Spritzschutz (16), der zwischen dem Auslassende (25) des Injektors (2) und der Lumineszenz-Optik (10) angeordnet ist.
摘要:
An ion concentration measurement system is provided. The system includes an oil mixture comprising an oil potentially containing at least one ion and at least one photo-responsive chemical that changes optical properties in the presence of at least one ion in the oil mixture, photo-detector circuitry configured to receive an optical property from the oil mixture, electronic conversion circuitry configured to convert the optical property into a value representing an ion concentration in the oil mixture, and electronic transmission circuitry configured to transmit a signal or data indicating the value. A method of measuring concentrations of at least one ion in an oil mixture is provided, including receiving at least one optical property from at least one photo-responsive chemical, converting the at least one optical property into a value representing an ion concentration in the oil mixture, and transmitting a signal or data comprising the value.
摘要:
The invention relates to a system (1) for optically detecting at least one substance in a fluid (200). The system (1) comprises particles (400, 410) arranged to be dispersed in the fluid (200), each one comprising a magnetic bead (400) and an agent (410). The agent (410) is reactive with said substance such that it gives a specific optical response upon a determined optical excitation or a chemical reaction. The system (1) further comprises a chamber (330) to contain said fluid (200) and particles (400, 410). The chamber (330) is closed at one side by a window (310) optically transparent to the wavelengths of said optical response and said optical excitation. The system (1) further comprises an optical detector (100) adapted to detect at least a portion of said optical response. The system (1) further comprises magnetic sources (103) arranged to magnetically move the particles (400, 410) onto or close to the window (310). The optical detector (100) is positioned to receive at least a portion of the optical response from the at least one agent (410) located onto or close to the window (310). The invention relates further to a disposable and a method.
摘要:
Invention describes a method and a device for testing toxicity of a test sample. The method comprises at least following steps: - providing an aliquot or a volume of light producing test reagent liquid in a reagent vial (22), - moistening a control swab (5) with the light producing test reagent liquid, - moistening a test swab (6) with the test sample, - moistening the test swab (6) with the light producing test reagent liquid, - reading luminescence of the control swab (5) in a luminometer and thereby obtaining a luminescence value of a control sample, - reading luminescence of the test swab (6) in the luminometer and obtaining a luminescence value of the test sample, - calculating the toxicity of the test sample by comparing the luminescence value of the control sample to the luminescence value of the test sample. According to the invention the moistenings of the control swab (5) and the test swab (6) with the light producing test reagent liquid are done with the same aliquot of light producing test reagent liquid in the same reagent vial (22).
摘要:
A chemical, biochemical or biological analysis system and method including: a surface acoustic wave (SAW) actuator (6) including a piezoelectric substrate (9) and at least one interdigital electrode (17) located on a working surface (11) of the piezoelectric substrate (9), the SAW actuator generating travelling SAWs in the working surface when an electrical signal is applied to the interdigital electrode; at least one reaction chamber (19) located on the working surface of the piezoelectric substrate; a light detector (16) for detecting luminescent, fluorescent or phosphorescent emissions within the reaction chamber; a reagent flow line (29) for providing a flow of reagent through the reaction chamber; a test sample supply line (29) for supplying a test sample to the reaction chamber; wherein the SAW actuator can generate travelling SAWs within the working surface to thereby induce chaotic micromixing, convective transport, concentration or combinations thereof of the test sample and the reagent contained within the reaction chamber.