公开(公告)号：WO2020026047A1

公开(公告)日：2020-02-06

申请号：PCT/IB2019/055211

申请日：2019-06-20

Applicant: INDIAN INSTITUTE OF SCIENCE

Inventor： KOTESA, Rahul Singh ,  ANANTHASURESH, G.k. ,  JHUNJHUNWALA,, Siddharth ,  SEN, Prosenjit

IPC: C12N5/00 ,  C12M3/00 ,  B01L3/00

Abstract: The present disclosure relates to a method for inducing enhanced poration in biological cells comprising: flowing a biological cell suspended in a medium through a microfluidic channel; introducing air through channels disposed in relation to the microfluidic channel in a cross-sectional manner, the air being introduced is to the extent to cause the biological cell to compress and thereby inducing enhanced poration in biological cell. The present invention in particular relates to a method for intracellular delivery of molecules or particulate matter by inducing enhanced poration in biological cell. The present disclosure further relates to a device for inducing enhanced poration in biological cell, more particularly for intracellular delivery of molecules or particles into the cell especially > 100 nm in size.

公开(公告)号：WO2018207087A1

公开(公告)日：2018-11-15

申请号：PCT/IB2018/053181

申请日：2018-05-08

Applicant: INDIAN INSTITUTE OF SCIENCE

Inventor： KOTESA, Rahul Singh ,  SEN, Prosenjit

IPC: G01N33/48

CPC classification number: G01N33/5026 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0663 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2400/0487 ,  C12M23/16 ,  G01N15/147 ,  G01N33/48 ,  G01N2015/0073 ,  G01N2015/1006

Abstract: The present disclosure relates to a new and improved microfluidic system which can precisely measure mechanical properties of a biological system such as single cell. The microfluidic system disclosed herein can enable measurement of cell mechanics at high throughputs, and can solve the channel clogging problem inherent in conventional bio-microfluidic devices. Further, the disclosed microfluidic system can eliminate cell-substrate interactions and provide more accurate estimate of mechanical properties of biological system. In an aspect, present disclosure provides a microfluidic system for determining mechanical properties of biological cells through single cell compression, wherein the system can include (a) a first microfluidic channel including an inlet and an outlet, the inlet being configured to receive a fluidic medium containing cells into the first microfluidic channel, and the outlet being configured to dispense the fluidic medium from the first microfluidic channel; and (b) a second microfluidic channel including a first air inlet and a second opposite air inlet; wherein the first and second microfluidic channels intersect perpendicularly to each other to form an intersection region; wherein each of the first and second air inlets receives pressurized air that flows towards the intersection region, and wherein the air flow creates, in the intersection region, an air/liquid interface which squeezes the cells as they pass through the air/liquid interface.