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    A TECHNIQUE FOR ALIGNING A NON-SPHERICAL BIOLOGICAL ENTITY IN A FLOW CELL
    1.
    发明申请
    A TECHNIQUE FOR ALIGNING A NON-SPHERICAL BIOLOGICAL ENTITY IN A FLOW CELL 审中-公开
    用于排除流动细胞中非球形生物学实体的技术

    公开(公告)号：WO2017054839A1

    公开(公告)日：2017-04-06

    申请号：PCT/EP2015/072273

    申请日：2015-09-28

    申请人： SIEMENS HEALTHCARE GMBH

    发明人： HAYDEN, Oliver ,  RICHTER, Lukas ,  UGELE, Matthias

    IPC分类号： G01N15/02 ,  G01N15/14 ,  B01L3/00 ,  G01N15/00

    CPC分类号： G01N15/0227 ,  B01L3/502776 ,  B01L2200/0663 ,  B01L2300/0877 ,  B01L2400/0436 ,  G01N15/1404 ,  G01N2015/0065 ,  G01N2015/0233 ,  G01N2015/1413 ,  G01N2015/142

    摘要： A technique is presented for aligning, in a desired region within a flow chamber of a flow cell, a non-spherical biological entity carried in a sample. The flow chamber has a rectangular cross-section. A bottom flow input module, a top flow input module and a sample input module provide a first fluid, a second fluid, and the sample, respectively, to the flow chamber. The first and the second fluids laminarly flow along a bottom and a top wall of the flow chamber and the sample laminarly flows sandwiched between them. By controlling rate of flow of the first and/or the second fluid the sample flow, and thus the non-spherical biological entity, is focused in the desired region. An acoustic transducer generates a standing acoustic wave having pressure node linearly arranged along an axis passing through the desired region to orient the non-spherical biological entity in the desired region.

    摘要翻译： 提出了一种在流动池的流动室内的期望区域中对准在样品中携带的非球形生物实体的技术。 流动室具有矩形横截面。 底部流量输入模块，顶部流量输入模块和样品输入模块分别向流动室提供第一流体，第二流体和样品。 第一和第二流体沿着流动室的底部和顶壁层流并且样品层流地夹在它们之间。 通过控制第一和/或第二流体的流速，样品流以及因此非球形生物实体被聚焦在所需区域中。 声换能器产生具有沿着通过所需区域的轴线直线排列的压力节点的驻声波，以将非球形生物实体定向在期望的区域。

    PHOTOCLEAVAGE METHOD AND APPARATUS TO CLEAN FLUIDIC DEVICES
    2.
    发明申请
    PHOTOCLEAVAGE METHOD AND APPARATUS TO CLEAN FLUIDIC DEVICES 审中-公开
    清洗流体装置的照相方法和装置

    公开(公告)号：WO2016036647A1

    公开(公告)日：2016-03-10

    申请号：PCT/US2015/047688

    申请日：2015-08-31

    申请人： BIONANO GENOMICS, INC.

    发明人： YAO, David, Xian, Wei ,  RIDGEWAY, William, K.

    IPC分类号： B01L3/00 ,  B08B7/00 ,  C12Q1/68

    CPC分类号： B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0896 ,  B01L2400/0406 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/086 ,  B08B7/00 ,  C12Q1/68

    摘要： A method and system for improving throughput of a fluidic system such as a biopolymer analysis system by cleaning accumulated or clogging biopolymer from the fluidic system is disclosed. The method and system utilize a light energy source to photocleave the biopolymer molecules that may accumulate or aggregate in the fluidic system or clog a passageway. The accumulated biopolymer may be exposed to a light energy source for a sufficient period of time such that the biopolymer molecule is dosed with sufficient energy to photocleave the biopolymer molecules, thereby restoring the efficiency of and flow through the system.

    摘要翻译： 公开了一种用于通过从流体系统中清洗积聚或堵塞生物聚合物来改善诸如生物聚合物分析系统的流体系统的生产量的方法和系统。 该方法和系统利用光能源来光洗可能在流体系统中积聚或聚集的生物聚合物分子或堵塞通道。 累积的生物聚合物可以暴露于光能源足够长的时间，使得生物聚合物分子被赋予足够的能量以使生物聚合物分子光滑，从而恢复系统的效率并流过系统。

    TWO DIMENSIONAL NANOFLUIDIC CCD ARRAYS FOR MANIPULATION OF CHARGED MOLECULES IN SOLUTION
    3.
    发明申请
    TWO DIMENSIONAL NANOFLUIDIC CCD ARRAYS FOR MANIPULATION OF CHARGED MOLECULES IN SOLUTION 审中-公开
    用于在解决方案中处理充电分子的二维纳米级CCD阵列

    公开(公告)号：WO2013101672A3

    公开(公告)日：2015-01-08

    申请号：PCT/US2012071004

    申请日：2012-12-20

    申请人： AGILENT TECHNOLOGIES INC ,  PETER BRIAN JON ,  MANNION JOHN T ,  YAMADA ALICE

    发明人： PETER BRIAN JON ,  MANNION JOHN T ,  YAMADA ALICE

    IPC分类号： C12M1/42

    CPC分类号： G01N27/44791 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0472

    摘要： Methods and apparatus for manipulation of charged molecules in solution. More particularly, the invention provides nanofluidic CCD arrays that are capable of manipulate one or a group of molecules on an individual bases such that they undergo controlled physical and/or chemical movements and/or transformations. The nanofluidic apparatus and methods that are capable of individualized movement and controlled manipulation of a molecule or group of molecules in solution environment. A key feature of the nanofluidic CCD array technology disclosed herein is that molecules or nanoparticles, in particular DNA and other large biomolecules, can be individually manipulated and moved to spatially distinctive parts of the nanofluidic device, without the use of electrophoresis or pressure. A nanofluidic CCD array can be constructed such that different regions of the array have different biochemical activities. Different DNA molecules in a single nanofluidic device can be addressed and treated individually, all without the use of valves.

    摘要翻译： 在溶液中操作带电分子的方法和装置。 更具体地，本发明提供纳米流体CCD阵列，其能够操纵单个基底上的一个或一组分子，使得它们经受受控的物理和/或化学运动和/或转化。 纳米流体装置和方法能够在溶液环境中分子或分子组的个性化运动和受控操纵。 本文公开的纳米流体CCD阵列技术的关键特征是分子或纳米颗粒，特别是DNA和其他大的生物分子，可以单独操作并移动到纳米流体装置的空间上独特的部分，而不使用电泳或压力。 可以构建纳米流体CCD阵列，使得阵列的不同区域具有不同的生物化学活性。 单个纳米流体装置中的不同DNA分子可以单独解决和处理，而不使用阀门。

    DEVICES AND METHODS FOR NUCLEIC ACID PREPARATION AND ANALYSIS
    4.
    发明申请
    DEVICES AND METHODS FOR NUCLEIC ACID PREPARATION AND ANALYSIS 审中-公开
    用于核酸制备和分析的装置和方法

    公开(公告)号：WO2013158860A1

    公开(公告)日：2013-10-24

    申请号：PCT/US2013/037140

    申请日：2013-04-18

    申请人： PATHOGENETIX, INC.

    发明人： HARRIS, John, L. ,  WEST, Adrian Mark, Thomas ,  TEN BROECK, Dirk, Peter ,  PROTOZANOVA, Ekaterina ,  MELTZER, Robert, H. ,  GRIFFIS, Joshua, W. ,  GILMANSHIN, Rudolf

    IPC分类号： C12Q1/68 ,  C12N15/11 ,  C12N15/10 ,  G01N35/08

    CPC分类号： C12Q1/6816 ,  B01L2200/0636 ,  B01L2200/0663 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/049 ,  C12Q2523/303 ,  C12Q2563/173 ,  C12Q2565/629

    摘要： The invention provides in part a device for preparing a fluid sample, including but not limited to a sample comprising genomic DNA. The invention also provides in part structures and methods that allow polymers of any length, including nucleic acids, to be stretched into a long, linear conformation for further analysis. The invention also provides in part devices and methods for improving labeling of nucleic acids including intercalation of nucleic acids using for example mono intercalators such as mono cyanine intercalators. The invention also provides in part methods for analyzing populations nucleic acids including nucleic acids from different subjects or different samples such as bacteria in biomes, and for identifying nucleic acids present in such populations. A non-limiting example is an analysis of bacteria in biomes. Another example is an analysis of DNA from different human subjects.

    摘要翻译： 本发明部分地提供了用于制备流体样品的装置，包括但不限于包含基因组DNA的样品。 本发明还提供部分结构和方法，使任何长度的聚合物（包括核酸）拉伸成长的线性构象用于进一步分析。 本发明还部分提供用于改进核酸标记的装置和方法，包括使用例如单嵌段剂如单花青嵌入剂插入核酸。 本发明还部分地提供了用于分析人群核酸的方法，所述核酸包括来自不同受试者或不同样品的生物样品中的细菌的核酸，以及用于鉴定存在于这些群体中的核酸。 非限制性实例是对生物群落中细菌的分析。 另一个例子是来自不同人类受试者的DNA的分析。

    NANO-PCR: METHODS AND DEVICES FOR NUCLEIC ACID AMPLIFICATION AND DETECTION
    8.
    发明申请
    NANO-PCR: METHODS AND DEVICES FOR NUCLEIC ACID AMPLIFICATION AND DETECTION 审中-公开
    NANO-PCR：用于核酸扩增和检测的方法和装置

    公开(公告)号：WO2006076022A3

    公开(公告)日：2007-12-06

    申请号：PCT/US2005016638

    申请日：2005-05-13

    申请人： GOEL ANITA

    发明人： GOEL ANITA

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： B01L7/52 ,  A61K41/00 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0663 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0454 ,  B01L2400/0487 ,  B82Y5/00 ,  B82Y30/00 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2523/307 ,  C12Q2527/101

    摘要： Methods, devices, and compositions are described that provide for amplification of nucleic acid sequences without reliance upon temperature cycling, thus freeing the methods from conventional benchtop thermal cycling devices. Denaturation of double stranded nucleic acids, primer annealing, and precision control over primer extension by polymerase can be accomplished by applying stress to a nucleic acid. These methods can provide one ore more benefits over conventional PCR methods including: precision control over the PCR process; generally improved fidelity; improved accuracy over problematic sequences such as GC-rich or tandem repeat regions; greater sequence length; increased reaction yield; reduced experimental time; greater efficiency; lower cost; greater portability; and, robustness to various environmental parameters, such as temperature, pH, and ionic strengths.

    摘要翻译： 描述了提供核酸序列的扩增而不依赖于温度循环的方法，装置和组合物，从而从常规台式热循环装置释放方法。 双链核酸的变性，引物退火和通过聚合酶引物延伸的精确控制可以通过对核酸施加应激来实现。 这些方法可以比常规PCR方法提供一个以上的益处，包括：精确控制PCR过程; 一般提高保真度; 提高了有问题的序列如GC富集或串联重复区域的准确性; 更长的序列长度; 增加反应产率; 减少实验时间; 更高的效率; 降低成本; 更大的便携性 以及对各种环境参数如温度，pH和离子强度的鲁棒性。

    MANIPULATION OF POLYMERS IN A MICROCHANNEL
    9.
    发明申请
    MANIPULATION OF POLYMERS IN A MICROCHANNEL 审中-公开
    聚合物在微通道中的操作

    公开(公告)号：WO2004091795A3

    公开(公告)日：2005-05-06

    申请号：PCT/US2004011664

    申请日：2004-04-08

    申请人： US GENOMICS INC ,  FUCHS MARTIN ,  LARSON JONATHAN

    发明人： FUCHS MARTIN ,  LARSON JONATHAN

    IPC分类号： B01L3/00 ,  G01N15/10 ,  G01N15/14 ,  G01N35/00 ,  C12Q1/68

    CPC分类号： G01N35/00069 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0663 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0861 ,  B01L2400/0463 ,  B01L2400/0487 ,  B01L2400/086 ,  G01N15/1475 ,  G01N2015/1006 ,  G01N2015/1413

    摘要： A microfluidic device for altering the flow of a carrier fluid containing a polymer, such that, the polymer can be positioned, aligned, or elongated. The device accomplishes these effects by directing the carrier fluid in a laminar flow into obstacles, or other fluids to knowingly alter the path of the carrier fluid streamlines. These streamlines, in turn, apply fluidic drag forces against the polymer to manipulate it into a desired configuration. Other aspects of the device retain a polymer in an aligned or elongated state with crimps which prevent portions of the polymer from coiling. These structures utilize the natural concept of increasing entropy to allow small portions of an aligned or elongated polymer to return to a high entropy, or coiled state, while retaining the majority of the polymer in a low entropy, aligned or elongated state for subsequent analysis or manipulation.

    摘要翻译： 一种用于改变含有聚合物的载体流体的流动的微流体装置，使得聚合物可以定位，对准或延长。 该装置通过将层流中的载体流引导到障碍物或其它流体中以明知地改变载体流体流线的路径来实现这些效果。 这些流线依次将流体阻力施加到聚合物上以将其操纵成所需的构型。 该装置的其它方面将聚合物保持在具有卷曲的对准或细长状态，防止聚合物部分卷取。 这些结构利用增加熵的自然概念，以允许对准或细长的聚合物的小部分返回到高熵或卷曲状态，同时将大部分聚合物保持在低熵，对准或细长状态用于随后的分析或 操纵。