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公开(公告)号：WO2018183988A1
公开(公告)日：2018-10-04
申请号：PCT/US2018/025611
申请日：2018-03-31
Applicant: INDIANA BIOSCIENCES RESEARCH INSTITUTE
Inventor： PUGIA, Michael, Joseph , BAIRD, Zane , CAO, Zehui
IPC: C12Q1/68 , C12Q1/6834 , C12Q1/6844 , C12Q1/6851
CPC classification number: C12Q1/682 , C12N15/1096 , C12Q1/6806 , C12Q2561/101 , C12Q2561/113 , C12Q2563/107 , C12Q2563/131 , C12Q2531/113 , C12Q2537/159
Abstract: Methods are provided for the selective isolation, amplification and detection of nucleic acids from samples, said method comprising: (a) enriching selectively said nucleic acids present in said samples on a binding matrix;(b) releasing said nucleic acids from the binding matrix; (c) selectively amplifying said nucleic acids; and (d) analysing said amplified nucleic acids.
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公开(公告)号：WO2018183989A1
公开(公告)日：2018-10-04
申请号：PCT/US2018/025613
申请日：2018-04-01
Applicant: INDIANA BIOSCIENCES RESEARCH INSTITUTE
Inventor： PUGIA, Michael, Joseph , BAIRD, Zane , CAO, Zehui
Abstract: The invention described herein is directed to methods of isolation of all variations of analyte in a sample by binding variations to a particle with attached analytical labels and separating the particles from the sample followed by removing analytical labels from particle and measuring the analyte molecules by the measuring the analytical labels. The separated analytical labels on the particle are then able to be used to measure the variations of analyte binding variations.
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公开(公告)号：WO2018183985A1
公开(公告)日：2018-10-04
申请号：PCT/US2018/025606
申请日：2018-03-31
Applicant: INDIANA BIOSCIENCES RESEARCH INSTITUTE
Inventor： PUGIA, Michael, Joseph , BAIRD, Zane , CAO, Zehui
CPC classification number: G01N1/4077 , B01L3/50273 , B01L3/5055 , B01L2200/0647 , B01L2300/0681 , B01L2400/0457 , B01L2400/0463 , B01L2400/049 , G01N27/3277 , G01N2001/4088
Abstract: The invention provides a method of releasing a liquid from a porous matrix having at least one pore to a microfluidic surface having at least one liquid volume area and at least one exit hole, comprising: (a) filtering said liquid through said porous matrix; (b) removing said porous matrix and sealing the microfluidic surface having a liquid volume area; (c) releasing said liquid from the liquid volume area through the exit hole of the microfluidic surface by application of a dynamic force; and (d) collecting said liquid into a liquid receiving area. Additionally, there is provided an apparatus useful for processing liquid samples undergoing analytical assays which includes (a) a filtering device having a porous matrix afixed to a support structure; (b) a microfluidic surface having a liquid volume area and an exit hole connected to said filtering device; and (c) a liquid receiving area attached to said microfluidic surface.
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