公开(公告)号：WO2009076778A1

公开(公告)日：2009-06-25

申请号：PCT/CA2009/000032

申请日：2009-01-12

Applicant: MEDICAGO INC. ,  D'AOUST, Marc-André ,  COUTURE, Manon ,  ORS, Frédéric ,  TRÉPANIER, Sonia ,  LAVOIE, Pierre-Olivier ,  DARGIS, Michèle ,  VÉZINA, Louis-Philippe ,  LANDRY, Nathalie

Inventor： D'AOUST, Marc-André ,  COUTURE, Manon ,  ORS, Frédéric ,  TRÉPANIER, Sonia ,  LAVOIE, Pierre-Olivier ,  DARGIS, Michèle ,  VÉZINA, Louis-Philippe ,  LANDRY, Nathalie

IPC: C12N15/82 ,  A01H5/00 ,  A61K35/76 ,  A61K39/145 ,  A61P31/16 ,  A61P37/04 ,  C07K14/11 ,  C12N7/00 ,  C12N15/44 ,  C12N7/01

CPC classification number: C12N15/8258 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/5258 ,  A61K2039/543 ,  A61K2039/55505 ,  A61K2039/55583 ,  A61K2039/55588 ,  A61K2039/58 ,  C07K14/005 ,  C07K2319/02 ,  C07K2319/03 ,  C12N7/00 ,  C12N9/90 ,  C12N15/8257 ,  C12N2760/16022 ,  C12N2760/16122 ,  C12N2760/16123 ,  C12N2760/16133 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16171 ,  C12N2760/16222 ,  C12N2760/16223 ,  C12N2760/16234 ,  C12Y503/04001 ,  G06F9/505 ,  G06F2209/508

Abstract: A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.

Abstract translation: 提供了用于在植物或植物的一部分内合成流感病毒样颗粒（VLP）的方法。 该方法涉及在流感病毒中表达流感HA并通过尺寸排阻色谱法进行纯化。 本发明还涉及包含流感HA蛋白和植物脂质的VLP。 本发明还涉及编码流感HA的核酸以及载体。 VLP可用于配制流感疫苗，或可用于丰富现有疫苗。

公开(公告)号：WO2013177701A1

公开(公告)日：2013-12-05

申请号：PCT/CA2013/050406

申请日：2013-05-28

Applicant: MEDICAGO INC.

Inventor： VÉZINA, Louis-Philippe ,  BECHTOLD, Nicole ,  LAURIN, Luc

IPC: A01G9/02

CPC classification number: A01G9/02 ,  A01G9/0297

Abstract: A plant tray for receiving and retaining one or more plant pots includes a plant- receiving tray for use in a high-throughput plant infiltration process used to produce recombinant proteins. The plant tray described has a base retaining tray with an upper surface defining cavities for receiving the plant pots, and a top plate displaceably mounted on the base retaining tray and having apertures, the apertures defining a channel with the corresponding cavities of the bottom tray when aligned. The top plate is displaceable between a locked operative position wherein the apertures and the corresponding cavities are partially misaligned, thereby locking the plant pots in between the top plate and the base retaining tray.

Abstract translation: 用于接收和保留一个或多个植物盆的植物托盘包括用于生产重组蛋白质的高通量植物浸润过程中的植物接收盘。 所描述的植物托盘具有基部保持托盘，其具有限定用于容纳植物盆的空腔的上表面，以及可移动地安装在基部保持托盘上并具有孔的顶板，所述孔限定与底托盘的相应空腔的通道， 对齐。 顶板可在锁定的操作位置之间移动，其中孔和相应的空腔部分地不对准，从而将植物盆锁定在顶板和基座保持盘之间。

公开(公告)号：WO2010006452A1

公开(公告)日：2010-01-21

申请号：PCT/CA2009/001040

申请日：2009-07-15

Applicant: MEDICAGO INC. ,  COUTURE, Manon ,  VÉZINA, Louis-Philippe ,  LANDRY, Nathalie

Inventor： COUTURE, Manon ,  VÉZINA, Louis-Philippe ,  LANDRY, Nathalie

IPC: C12N15/14 ,  A01H5/00 ,  A61K39/145 ,  A61P31/16 ,  C07K14/11 ,  C12N15/63 ,  C12N15/82 ,  C12N5/10 ,  C12N7/00 ,  C12N7/01 ,  A61J1/05 ,  A61M5/178

CPC classification number: C12N15/8257 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/517 ,  A61K2039/5258 ,  A61K2039/543 ,  C07K16/1018 ,  C12N7/00 ,  C12N2760/16123 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16223 ,  C12N2760/16234 ,  C12N2760/16251

Abstract: A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of a novel influenza HA protein in plants and its purification The invention is also directed towards a VLP comprising influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding improved influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.

Abstract translation: 提供了在植物或植物的一部分内合成流感病毒样颗粒（VLP）的方法。 该方法包括在植物中表达新型流感HA蛋白及其纯化本发明还涉及包含流感HA蛋白和植物脂质的VLP。 本发明还涉及编码改进的流感HA以及载体的核酸。 VLP可用于制定流感疫苗，或可用于丰富现有疫苗。