公开(公告)号：WO2015161914A3

公开(公告)日：2016-03-10

申请号：PCT/EP2015000679

申请日：2015-03-30

Applicant: MERCK PATENT GMBH

Inventor： FELDEN LUC ,  BOUTHILLON MARINE ,  OLIVIER STÉPHANE ,  WOEHL PIERRE ,  GUEDON PIERRE ,  ALLARD FLORIAN

IPC: G06K9/00 ,  G01N21/17 ,  G01N21/25 ,  G01N21/47 ,  G06T7/00

CPC classification number: C12Q1/04 ,  C12Q1/22 ,  G01N21/47 ,  G01N21/55 ,  G01N2021/1765 ,  G01N2021/4704 ,  G01N2021/4709 ,  G01N2021/4711 ,  G01N2201/103 ,  G06K9/00134 ,  G06T7/0012 ,  G06T2207/10012 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: The present invention is directed to a method and an apparatus for detecting micro-colonies growing on a membrane (1) or an agarose medium of a sample (5) in a closed device (3). According to the invention the sample (5) is irradiated with a light incident at an angle (β) with respect to the normal to the membrane (1) or the surface of the agarose medium from outside the device (3). An incident area (7) of the light on the membrane (1) or the surface of the agarose medium is imaged by means of a light receiving element (9) using an imaging angle (α) different from angle (β) with respect to the normal to the membrane (1) or the surface of the agarose medium from outside the device (3). The light reflected, scattered and/ or diffused from the membrane or the surface of the agarose medium and/or the micro-colonies on the membrane and/or the micro-colonies on the agarose medium is detected.

Abstract translation: 本发明涉及用于检测在封闭装置（3）中样品（5）的膜（1）或琼脂糖培养基上生长的微菌落的方法和设备。 根据本发明，用来自装置（3）外部的相对于膜（1）的法线或琼脂糖介质的表面以角度（β）入射的光照射样品（5）。 使用与角度（β）不同的成像角度（α）相对于光接收元件（9）利用光接收元件（9）成像膜（1）上的光或琼脂糖介质表面的入射区域（7） 膜（1）的法线或来自装置（3）外部的琼脂糖介质的表面。 检测从膜或琼脂糖培养基表面和/或膜上的微菌落和/或琼脂糖培养基上的微菌落反射，散射和/或扩散的光。

公开(公告)号：WO2015161914A2

公开(公告)日：2015-10-29

申请号：PCT/EP2015000679

申请日：2015-03-30

Applicant: MERCK PATENT GMBH

Inventor： FELDEN LUC ,  BOUTHILLON MARINE ,  OLIVIER STÉPHANE ,  WHOEHL PIERRE ,  GUEDON PIERRE ,  ALLARD FLORIAN

IPC: G01N9/00

CPC classification number: C12Q1/04 ,  C12Q1/22 ,  G01N21/47 ,  G01N21/55 ,  G01N2021/1765 ,  G01N2021/4704 ,  G01N2021/4709 ,  G01N2021/4711 ,  G01N2201/103 ,  G06K9/00134 ,  G06T7/0012 ,  G06T2207/10012 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: The present invention is directed to a method and an apparatus for detecting micro-colonies growing on a membrane (1) or an agarose medium of a sample (5) in a closed device (3). According to the invention the sample (5) is irradiated with a light incident at an angle (β) with respect to the normal to the membrane (1) or the surface of the agarose medium from outside the device (3). An incident area (7) of the light on the membrane (1) or the surface of the agarose medium is imaged by means of a light receiving element (9) using an imaging angle (α) different from angle (β) with respect to the normal to the membrane (1) or the surface of the agarose medium from outside the device (3). The light reflected, scattered and/ or diffused from the membrane or the surface of the agarose medium and/or the micro-colonies on the membrane and/or the micro-colonies on the agarose medium is detected.

Abstract translation: 本发明涉及一种用于检测在封闭装置（3）中在膜（1）或样品（5）的琼脂糖培养基上生长的微菌落的方法和装置。 根据本发明，从装置（3）外部以相对于膜（1）的法线或琼脂糖介质的表面以角度（β）入射的光照射样品（5）。 使用与角度（β）不同的成像角度（α），通过光接收元件（9）对膜（1）上的光或琼脂糖介质的表面的入射区域（7）成像， （1）的法线或琼脂糖介质的表面（3）。 检测从膜或琼脂糖介质的表面和/或膜上的微生物群和/或琼脂糖培养基上的微菌落反射，散射和/或扩散的光。