公开(公告)号：WO1987007384A1

公开(公告)日：1987-12-03

申请号：PCT/US1987000198

申请日：1987-02-02

申请人： QUIDEL

发明人： QUIDEL ,  WALSH, Michael, J.

IPC分类号： G01N33/53

CPC分类号： G01N33/53 ,  G01N33/54366

摘要： A device for concentrating enzyme immunoassay sandwich product in a very narrow band (14) in a column (10) for development and visual reading. The device may be constructed using a clear or transparent vessel or column (10), with a fluid inlet, in which there is disposed a band (14) or a plurality of bands (14) of small particles to which one of an immunochemical couple is bound, the other member of which couple is the species to be determined. The immunochemical band (14) is defined by bands (16, 18) of non-immunochemically active particles. An absorbent pad (23) may support the bands.

摘要翻译： 用于将酶免疫测定三明治产物浓缩在柱（10）中的非常窄的带（14）中用于显影和视觉阅读的装置。 该装置可以使用具有流体入口的透明或透明的容器或塔（10）来构造，其中设置有带（14）或多个小颗粒带（14），免疫化学对 是有约束力的，另外一对夫妇是要确定的物种。 免疫化学带（14）由非免疫化学活性颗粒的带（16,18）定义。 吸收垫（23）可以支撑带。

公开(公告)号：WO1986001221A1

公开(公告)日：1986-02-27

申请号：PCT/US1984002048

申请日：1984-12-31

申请人： QUIDEL

发明人： QUIDEL ,  MARQUIS, David, M. ,  SMOLEC, Jo, Marie

IPC分类号： C12N01/20

CPC分类号： C07K14/55

摘要： The cDNA which encodes TCGF (IL-2), plasmids containing the same, and E. coli which contain such plasmids and express such cDNA suitable for the production of TCGF.

公开(公告)号：WO1984004170A1

公开(公告)日：1984-10-25

申请号：PCT/US1983001953

申请日：1983-12-14

申请人： QUIDEL

发明人： QUIDEL ,  KATZ, David, H. ,  CHANG, Shung-Ho

IPC分类号： G01N33/54

CPC分类号： G01N33/563 ,  G01N33/531 ,  G01N33/56944 ,  G01N33/6857

摘要： Improved reagents and methods of preparing the same for the assay of Streptococcus Group A, and other antigen containing organisms, in which the high background resulting from cross-reaction of Anti-Streptococcal Group A or the reaction with Staphylococcus is eliminated, by chemically modifying the antibody wherein the reactivity of the antibody for the antigen is protected during such modification. Modifications include cleaving the Fc portion of the antibody and binding the antibody or a fragment thereof to a solid phase support.

摘要翻译： 改进的试剂及其制备方法用于测定链球菌A组和其他含抗原的生物，其中通过化学修饰抗链球菌A组或与葡萄球菌的反应而消除由链球菌A组交叉反应产生的高背景 抗体，其中抗体对抗原的反应性在这种修饰过程中被保护。 修饰包括切割抗体的Fc部分并将抗体或其片段与固相载体结合。

公开(公告)号：WO1984002004A1

公开(公告)日：1984-05-24

申请号：PCT/US1983001755

申请日：1983-11-08

申请人： QUIDEL

发明人： QUIDEL ,  BOHN, Joseph, W. ,  COHEN, Peter, A. ,  ZURAW, Bruce, L.

IPC分类号： G01N33/54

CPC分类号： B01L3/5021 ,  B01L3/502 ,  B01L3/5082 ,  B01L2300/0681 ,  B01L2400/0478 ,  G01N33/54366 ,  Y10S436/808 ,  Y10S436/81

摘要： A system for conducting an immunoassay in a single chamber, by concentrating antibodies for antigenic components on microbeads (190) treated for binding immunological components, physically separating the fluid from which the immunological active component is separated, and determining the amount of immunological component collected on the beads (190), comprising a multiple-chamber (A-D) dispensing cylinder (110).

摘要翻译： 一种用于在单个室中进行免疫测定的系统，通过将用于结合免疫组分处理的微珠上的抗原成分的抗体集中在物理上分离免疫活性成分被分离的流体，并测定在珠上收集的免疫组分的量， 包括多室分配缸。

公开(公告)号：WO1985002260A1

公开(公告)日：1985-05-23

申请号：PCT/US1983001757

申请日：1983-11-08

申请人： QUIDEL

发明人： QUIDEL ,  BOHN, Joseph, W. ,  COHEN, Peter, A. ,  ZURAW, Bruce, L.

IPC分类号： G01N33/54

CPC分类号： G01N33/5304 ,  G01N33/54313

摘要： A method and apparatus for conducting an immunoassay in a single chamber (10), by concentrating antibodies for antigenic components on microbeads (26) treated for binding immunological components, physically separating the fluid from which the immunological active component is separated, and determining the amount of immunological component collected on the beads (26), and an apparatus for carrying out such an immunoassay are disclosed.

摘要翻译： 一种用于在单个室（10）中进行免疫测定的方法和装置，通过将用于抗原成分的抗体集中在用于结合免疫组分处理的微珠（26）上，物理分离免疫活性成分被分离的流体，并确定量 收集在珠粒（26）上的免疫组分和用于进行这种免疫测定的装置。

公开(公告)号：WO1984004171A1

公开(公告)日：1984-10-25

申请号：PCT/US1983001954

申请日：1983-12-14

申请人： QUIDEL

发明人： QUIDEL ,  KATZ, David, H. ,  COOPER, Steven, W. ,  LEE, Theodore, T. ,  CHANG, Shung-Ho

IPC分类号： G01N33/54

CPC分类号： G01N33/54386 ,  G01N33/58 ,  G01N33/76 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/975 ,  Y10S436/808 ,  Y10S436/81 ,  Y10S436/818

摘要： Human chorionic gonadotropin (HCG) is detected by enzyme immunoassay by bonding biotinylated antibody to an avidin coupled paper disc, reacting the antibody on the disc with the solution suspected of containing HCG, and determining the amount of HCG on the disc by enzymatic assay detection techniques.

公开(公告)号：WO1987007145A1

公开(公告)日：1987-12-03

申请号：PCT/US1987000197

申请日：1987-02-02

申请人： QUIDEL

发明人： QUIDEL ,  KATZ, David, H.

IPC分类号： A61K37/02

CPC分类号： A61K51/088 ,  A61K47/645 ,  A61K2121/00

摘要： Receptor blocking technology using proteins conjugated to polymers of D-glutamic acid and D-lysine for treatment of antibody-mediated autoimmune disease, membrane and tumour disorders.

摘要翻译： 使用与D-谷氨酸和D-赖氨酸的聚合物缀合的蛋白质的受体阻断技术用于治疗抗体介导的自身免疫疾病，膜和肿瘤疾病。

公开(公告)号：WO1985000750A1

公开(公告)日：1985-02-28

申请号：PCT/US1984000594

申请日：1984-04-16

申请人： QUIDEL

发明人： QUIDEL ,  KATZ, David, H.

IPC分类号： A61K39/395

CPC分类号： C07K14/52 ,  A61K38/00 ,  C12N15/11

摘要： A monoclonal hybridoma cell line was constructed for the production of suppressive factor of allergy (SFA). mRNA isolated from the monoclonal hybridoma cell line was identified by its ability to direct SFA synthesis in Xenopus laevis oocytes. The SFA produced by the monoclonal hybridoma cell line and the translational product of the mRNA in the oocytes was found effective in inhibiting IgE-specific receptor expression in dilutions greater than 1:1,000 of the culture supernatant. A sensitive bioasay for SFA was developed using cultures of thymocytes in which Lyt-2 cells were blocked with monoclonal antibodies, and subsequent IgE-specific receptor expression was determined in Lyt-1 cells.

摘要翻译： 构建了用于产生过敏抑制因子（SFA）的单克隆杂交瘤细胞系。 通过其在非洲爪蟾卵母细胞中引导SFA合成的能力来鉴定从单克隆杂交瘤细胞系分离的mRNA。 发现由单克隆杂交瘤细胞系产生的SFA和卵母细胞中mRNA的翻译产物在抑制大于1:1000培养上清液的稀释度中的IgE特异性受体表达中有效。 使用胸腺细胞培养物开发SFA的敏感生物测定，其中Lyt-2细胞用单克隆抗体阻断，随后在Lyt-1细胞中测定IgE特异性受体表达。

公开(公告)号：WO1984004169A1

公开(公告)日：1984-10-25

申请号：PCT/US1983001877

申请日：1983-11-28

申请人： QUIDEL

发明人： QUIDEL ,  KATZ, David, H. ,  CHANG, Shung-Ho

IPC分类号： G01N33/54

CPC分类号： G01N33/531 ,  G01N33/563 ,  G01N33/56944 ,  G01N33/6857

摘要： An improved diagnostic method and reagent for the assay of Streptococcus Group A, and other antigen containing organisms, in which the high background resulting from cross-reaction of Anti-Streptococcal Group A or the reaction with Staphylococcus is eliminated.

摘要翻译： 用于测定链球菌A组和其他含抗原的生物体的改进的诊断方法和试剂，其中消除了抗链球菌A组交叉反应或与葡萄球菌反应产生的高背景。