公开(公告)号：WO2021154791A1

公开(公告)日：2021-08-05

申请号：PCT/US2021/015192

申请日：2021-01-27

Applicant: REGENERON PHARMACEUTICALS, INC.

Inventor： CHENG, Xiping ,  ROJAS, Jose F. ,  SLEEMAN, Mark

IPC: A01K67/027 ,  C12N15/90 ,  C12N15/11 ,  C12N9/14

Abstract: Non-human animal genomes, non-human animal cells, and non-human animals comprising a humanized PNPLA3 locus and methods of making and using such non-human animal genomes, non-human animal cells, and non-human animals are provided. Non-human animal cells or non-human animals comprising a humanized PNPLA3 locus express a human PNPLA3 protein or a chimeric PNPLA3 protein, fragments of which are from human PNPLA3. Methods are provided for using such non-human animals comprising a humanized PNPLA3 locus to assess in vivo efficacy of human -PNPL A3 -targeting reagents such as nuclease agents designed to target human PNPLA3.

公开(公告)号：WO2020206134A1

公开(公告)日：2020-10-08

申请号：PCT/US2020/026405

申请日：2020-04-02

Applicant: REGENERON PHARMACEUTICALS, INC.

Inventor： BRYDGES, Susannah ,  ROJAS, Jose F. ,  WARSHAW, Gregg S. ,  SIAO, Chia-Jen

IPC: C12N15/10 ,  C12N15/64

Abstract: Methods for introducing a scarless targeted genetic modification into a preexisting targeting vector are provided. The methods can use combinations of bacterial homologous recombination (BHR) and in vitro assembly to introduce such targeted genetic modifications into a preexisting targeting vector in a scarless manner.

公开(公告)号：WO2015057758A1

公开(公告)日：2015-04-23

申请号：PCT/US2014/060568

申请日：2014-10-15

Applicant: REGENERON PHARMACEUTICALS, INC.

Inventor： ROJAS, Jose F. ,  LAI, Ka-Man Venus ,  MURPHY, Andrew J.

IPC: A01K67/027 ,  C07K14/54

CPC classification number: A01K67/0278 ,  A01K2207/15 ,  A01K2217/07 ,  A01K2217/072 ,  A01K2227/10 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0387 ,  C07K14/5443 ,  C12N15/8509 ,  C12N2015/8527

Abstract: Genetically modified non-human animals comprising a humanized interleukin-15 (IL-15) gene. Cells, embryos, and non-human animals comprising a human IL-15 gene. Rodents that express humanized or human IL-15 protein.

Abstract translation: 包含人源白细胞介素-15（IL-15）基因的转基因非人动物。 包含人IL-15基因的细胞，胚胎和非人动物。 表达人源化或人类IL-15蛋白的啮齿动物。