公开(公告)号：WO2017005586A1

公开(公告)日：2017-01-12

申请号：PCT/EP2016/065249

申请日：2016-06-30

Applicant: ROBERT BOSCH GMBH

Inventor： MARUNIAK, Autumn ,  AHMAD, Habib ,  RAGHUNATHAN, Ashwin ,  JOHNSON, Christopher ,  KAVUSI, Sam ,  FOMINA, Nadezda ,  LANG, Christoph

IPC: G05D21/02 ,  G01N27/416 ,  G01N27/30

CPC classification number: G01N27/4167 ,  C12Q1/001 ,  G01N27/302 ,  G01N27/34 ,  G01N27/36 ,  G05D21/02

Abstract: Device and methods for use in a biosensor comprising a multisite array of test sites, the device and methods being useful for modulating the binding interactions between a (biomolecular) probe or detection agent and an analyte of interest by modulating the pH or ionic gradient near the electrodes in such biosensor. An electrochemically active agent that is suitable for use in biological buffers for changing the pH of the biological buffers. Method for changing the pH of biological buffers using the electrochemically active agents. The methods of modulating the binding interactions provided in a biosensor, analytic methods for more accurately controlling and measuring the pH or ionic gradient near the electrodes in such biosensor, and analytic methods for more accurately measuring an analyte of interest in a biological sample.

Abstract translation: 用于生物传感器的装置和方法，包括测试点的多位阵列，所述装置和方法可用于通过调节（生物分子）探针或检测剂与所分析物之间的结合相互作用，通过调节附近的pH或离子梯度 这种生物传感器中的电极。 适用于改变生物缓冲液pH值的生物缓冲液中的电化学活性剂。 使用电化学活性剂改变生物缓冲液的pH的方法。 调节生物传感器中提供的结合相互作用的方法，用于更准确地控制和测量这些生物传感器中电极附近的pH或离子梯度的分析方法，以及用于更精确地测量生物样品中感兴趣分析物的分析方法。

公开(公告)号：WO2022016087A1

公开(公告)日：2022-01-20

申请号：PCT/US2021/042031

申请日：2021-07-16

Applicant: ROBERT BOSCH GMBH ,  JOHNSON, Christopher ,  KAVUSI, Sam ,  FOMINA, Nadezda ,  AHMAD, Habib ,  MARUNIAK, Autumn ,  LANG, Christoph ,  RAGHUNATHAN, Ashwin ,  SHIN, Young Shik ,  DARVISH, Armin ,  PAPAGEORGIOU, Efthymios

Inventor： JOHNSON, Christopher ,  KAVUSI, Sam ,  FOMINA, Nadezda ,  AHMAD, Habib ,  MARUNIAK, Autumn ,  LANG, Christoph ,  RAGHUNATHAN, Ashwin ,  SHIN, Young Shik ,  DARVISH, Armin ,  PAPAGEORGIOU, Efthymios

IPC: C12Q1/00 ,  G01N27/30 ,  G01N27/34

Abstract: Device and methods for controlling pH or ionic gradient comprising a multisite array of feedback electrode sets comprising electrodes and pH sensing elements. The electrodes can include a reference electrode, counter electrode, and a working electrode. The device and methods iteratively select an amount of current and/or voltage to be applied to each working electrode, apply the selected amount of current and/or voltage to each working electrode to change pH of a solution close to the working electrode, and measure the signal output of the sensing element. The multisite array can include feedback and non-feedback electrode sets.

公开(公告)号：WO2018002002A1

公开(公告)日：2018-01-04

申请号：PCT/EP2017/065777

申请日：2017-06-27

Applicant: ROBERT BOSCH GMBH

Inventor： MARUNIAK, Autumn ,  AHMAD, Habib ,  JOHNSON, Christopher ,  KAVUSI, Sam ,  FOMINA, Nadezda

IPC: G01N27/327 ,  C12Q1/00

Abstract: A coated electrode includes an electrode, a coating configured to immobilize biomolecules, and a coating configured to improve electron transfer rate. Methods of making the coated electrode are also provided. A biosensor comprises a plurality of electrodes, each electrode including the coated electrode.

Abstract translation: 涂覆的电极包括电极，构造成固定生物分子的涂层和构造成改善电子传递速率的涂层。 还提供了制造涂覆电极的方法。 生物传感器包括多个电极，每个电极包括涂覆的电极。

公开(公告)号：WO2017207298A1

公开(公告)日：2017-12-07

申请号：PCT/EP2017/062083

申请日：2017-05-19

Applicant: ROBERT BOSCH GMBH

Inventor： MARUNIAK, Autumn ,  AHMAD, Habib ,  JOHNSON, Christopher ,  KAVUSI, Sam ,  FOMINA, Nadezda

IPC: C12N11/00 ,  C08L1/00 ,  C08L3/00 ,  C08L5/00 ,  C08L23/00 ,  C08L33/12 ,  C08L33/26 ,  C08L71/02 ,  C08L77/04 ,  C08L79/02

Abstract: A bifunctional polymer is functionalized at one end with an azlactone end group to conjugate biomolecules of interest, and is functionalized at another end with an azide anchor group to attach the polymer to a substrate. Methods of making the bifunctional polymer are also provided. A coated substrate includes the bifunctionalized polymers on the surface of a substrate. Methods of making the coated substrate are also provided. A microarray includes a plurality of discrete regions, each region including the coated substrate.

Abstract translation: 双功能聚合物在一端用吖内酯端基官能化以缀合感兴趣的生物分子，并且在另一端用叠氮基锚定基团官能化以将聚合物附着到基底上。 还提供了制备双官能聚合物的方法。 涂覆的基材包括在基材表面上的双官能化聚合物。 还提供了制造涂覆基材的方法。 微阵列包括多个分立的区域，每个区域包括涂覆的基底。

公开(公告)号：WO2017005587A1

公开(公告)日：2017-01-12

申请号：PCT/EP2016/065252

申请日：2016-06-30

Applicant: ROBERT BOSCH GMBH

Inventor： MARUNIAK, Autumn ,  AHMAD, Habib ,  RAGHUNATHAN, Ashwin ,  JOHNSON, Christopher ,  KAVUSI, Sam ,  FOMINA, Nadezda ,  LANG, Christoph

IPC: C12Q1/00 ,  G01N33/543 ,  C07C37/00

CPC classification number: C07C49/84 ,  C07C37/00 ,  C07C37/07 ,  C07C39/10 ,  C07C39/11 ,  C07C41/01 ,  C07C41/24 ,  C07C41/30 ,  C07C43/1783 ,  C07C43/2055 ,  C07C43/225 ,  C07C43/23 ,  C07C45/42 ,  C07C46/06 ,  C07C49/82 ,  C12Q1/001 ,  C12Q1/004 ,  G01N27/302 ,  G01N27/327 ,  G01N27/3275 ,  G01N27/4163 ,  G01N27/4167 ,  G01N33/5438

Abstract: Device and methods for use in a biosensor comprising a multisite array of test sites, the device and methods being useful for modulating the binding interactions between a (biomolecular) probe or detection agent and an analyte of interest by modulating the pH or ionic gradient near the electrodes in such biosensor. An electrochemically active agent comprising quinone derivatives that is suitable for use in biological buffers for changing the pH of the biological buffers. Method for changing the pH of biological buffers using the electrochemically active agents. The methods of modulating the binding interactions provided in a biosensor, analytic methods for more accurately controlling and measuring the pH or ionic gradient near the electrodes in such biosensor, and analytic methods for more accurately measuring an analyte of interest in a biological sample.

Abstract translation: 用于生物传感器的装置和方法，包括测试点的多位阵列，所述装置和方法可用于通过调节（生物分子）探针或检测剂与所分析物之间的结合相互作用，通过调节附近的pH或离子梯度 这种生物传感器中的电极。 包含适于用于改变生物缓冲液的pH的生物缓冲液中的醌衍生物的电化学活性剂。 使用电化学活性剂改变生物缓冲液的pH的方法。 调节生物传感器中提供的结合相互作用的方法，用于更准确地控制和测量这些生物传感器中电极附近的pH或离子梯度的分析方法，以及用于更精确地测量生物样品中感兴趣分析物的分析方法。