公开(公告)号：WO2016044655A2

公开(公告)日：2016-03-24

申请号：PCT/US2015050795

申请日：2015-09-17

申请人： SPOGEN BIOTECH INC

发明人： THOMPSON BRIAN ,  SIEGEL ASHLEY

IPC分类号： C07K14/32

CPC分类号： C07K14/32 ,  A01N37/44 ,  A01N63/02 ,  A23K10/18 ,  A23K20/147 ,  A61K39/00 ,  A61L2/18 ,  C02F3/342 ,  C02F3/348 ,  C02F2101/10 ,  C02F2101/30 ,  C02F2103/002 ,  C02F2103/003 ,  C02F2103/007 ,  C02F2103/06 ,  C02F2103/10 ,  C02F2103/14 ,  C02F2103/16 ,  C02F2103/26 ,  C02F2103/28 ,  C07K2319/01 ,  C07K2319/035 ,  C07K2319/40 ,  C09K8/62 ,  C09K2208/24 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  C12P21/02 ,  E21B43/16

摘要： Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifiations of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

摘要翻译： 提供了融合蛋白，其含有靶向序列，外孢壁蛋白或将融合蛋白靶向蜡状芽孢杆菌家族成员的外孢壁的外孢壁蛋白片段。 还提供了表达这种融合蛋白的重组蜡状芽孢杆菌家族成员。 还提供了基因失活的蜡状芽孢杆菌家族成员和过表达外孢菌蛋白的重组蜡状芽孢杆菌家族成员。 还提供了用重组蜡状芽孢杆菌家族成员包被的种子和使用重组蜡状芽孢杆菌家族成员（例如，用于刺激植物生长）的方法。 进一步提供了表达融合蛋白的重组蜡状芽孢杆菌家族成员的各种修饰。 还提供了包含孢子外壳蛋白和感兴趣的蛋白质或肽的融合蛋白，表达此类融合蛋白的重组细菌，用此类重组细菌包被的种子，以及使用这种重组细菌的方法（例如用于刺激植物生长）。