公开(公告)号：WO2013122816A1

公开(公告)日：2013-08-22

申请号：PCT/US2013/025215

申请日：2013-02-07

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： KAMPMANN, Martin ,  BASSIK, Michael ,  WEISSMAN, Jonathan

IPC: C12Q1/68 ,  C12N15/11 ,  G01N33/50 ,  G01N33/58

CPC classification number: C12N15/1075 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/12 ,  C12Q1/68 ,  C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/142 ,  G01N33/502 ,  G06F19/18 ,  C12Q2525/207 ,  C12Q2525/301 ,  C12Q2535/122

Abstract: The present invention provides methods for conducting screens using nucleic acid elements (e.g., interfering RNAs) to confidently identify hit genetic elements. The present invention further comprises constructing vectors that contain two or more nucleic acid elements to knock down all pairwise combinations of the hit genetic elements identified from the screen. Following quantitation of the single and double-knockdown phenotypes, genetic interactions between all gene pairs can be calculated. Genes can then be clustered according to the similarity of the pattern of their interactions with all of the other genes to obtain a genetic interaction map, which can advantageously be used to predict functional associations between genes and identify drug targets for therapy such as combination cancer therapy.

Abstract translation: 本发明提供了使用核酸元件（例如干扰RNA）进行筛选以自信地鉴定命中遗传元件的方法。 本发明还包括构建含有两个或多个核酸元件的载体，以敲除从筛选鉴定的命中遗传元件的所有成对组合。 在单次和双重敲低表型的定量之后，可以计算所有基因对之间的遗传相互作用。 然后可以根据其与所有其他基因的相互作用模式的相似性将基因聚类，以获得遗传相互作用图，其可有利地用于预测基因之间的功能关联并鉴定用于治疗的药物靶标，例如组合癌症治疗 。

公开(公告)号：WO2016011080A2

公开(公告)日：2016-01-21

申请号：PCT/US2015/040449

申请日：2015-07-14

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： GILBERT, Luke A. ,  HORLBECK, Max ,  KAMPMANN, Martin ,  QI, Lei S. ,  WEISSMAN, Jonathan S.

IPC: C40B30/04

CPC classification number: C12N15/1079 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/63 ,  C12Q1/6897

Abstract: Methods, compositions, and kits are provided for CRISPR/Cas mediated transcriptional modulation.

Abstract translation: 提供方法，组合物和试剂盒用于CRISPR / Cas介导的转录调节。