-
公开(公告)号：WO2021248023A2
公开(公告)日：2021-12-09
申请号：PCT/US2021/035937
申请日：2021-06-04
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： GILBERT, Luke , WEISSMAN, Jonathan , NUNEZ, James , POMMIER, Greg
Abstract: Provided herein are, inter alia, compositions and methods for modulating gene expression.
-
公开(公告)号：WO2010062775A2
公开(公告)日：2010-06-03
申请号：PCT/US2009/063165
申请日：2009-11-03
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , WEISSMAN, Jonathan , INGOLIA, Nicholas , GHAEMMAGHAMI, Sina , NEWMAN, John
Inventor： WEISSMAN, Jonathan , INGOLIA, Nicholas , GHAEMMAGHAMI, Sina , NEWMAN, John
CPC classification number: C12N15/1041 , C12N15/1034 , C12N15/1093 , C12Q1/6809 , C40B40/08 , C40B50/06
Abstract: Methods are provided for, inter alia, detecting nucleic acid molecules resistant to degradation, such as a plurality of RNA molecules bound to a ribosome, using various technologies including deep sequencing.
Abstract translation: 提供的方法尤其是使用包括深度测序的各种技术来检测抗降解的核酸分子，例如与核糖体结合的多个RNA分子。
-
公开(公告)号：WO2019204766A1
公开(公告)日：2019-10-24
申请号：PCT/US2019/028377
申请日：2019-04-19
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： CHEN, Jin , GILBERT, Luke , NUNEZ, James , WEISSMAN, Jonathan
IPC: C12Q1/68 , C12N9/22 , C12N15/63 , C12N15/117 , C12N15/85
Abstract: Provided herein are, inter alia , compositions and methods for manipulation of genomes of living organisms.
-
公开(公告)号：WO2012074855A2
公开(公告)日：2012-06-07
申请号：PCT/US2011/061970
申请日：2011-11-22
Applicant: WEISSMAN, Jonathan , CHURCHMAN, Lee Stirling , THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： WEISSMAN, Jonathan , CHURCHMAN, Lee Stirling
CPC classification number: C12Q1/6869 , C12Q1/6806 , C12Q1/6809 , C12Q2521/107 , C12Q2527/101 , C12Q2535/122 , C12Q2565/113
Abstract: Methods and compositions for identifying a cellular nascent RNA transcript are provided.
Abstract translation: 提供用于鉴定细胞新生RNA转录物的方法和组合物。
-
公开(公告)号：WO2018112423A1
公开(公告)日：2018-06-21
申请号：PCT/US2017/066842
申请日：2017-12-15
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： GILBERT, Luke , HORLBECK, Maximilian A. , JOST, Marco , WEISSMAN, Jonathan
IPC: C12N15/63 , C12N15/113 , C12N15/90
Abstract: Provided herein are compositions and methods for CRISPR-based screening.
-
Patent Agency Ranking6.发明申请METHODS FOR GENOME-WIDE SCREENING AND CONSTRUCTION OF GENETIC INTERACTION MAPS 审中-公开用于基因型筛选和遗传相互作用的构建方法
公开(公告)号：WO2013122816A1
公开(公告)日：2013-08-22
申请号：PCT/US2013/025215
申请日：2013-02-07
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： KAMPMANN, Martin , BASSIK, Michael , WEISSMAN, Jonathan
CPC classification number: C12N15/1075 , C12N15/111 , C12N2310/14 , C12N2310/531 , C12N2320/12 , C12Q1/68 , C12Q1/6886 , C12Q2600/136 , C12Q2600/142 , G01N33/502 , G06F19/18 , C12Q2525/207 , C12Q2525/301 , C12Q2535/122
Abstract: The present invention provides methods for conducting screens using nucleic acid elements (e.g., interfering RNAs) to confidently identify hit genetic elements. The present invention further comprises constructing vectors that contain two or more nucleic acid elements to knock down all pairwise combinations of the hit genetic elements identified from the screen. Following quantitation of the single and double-knockdown phenotypes, genetic interactions between all gene pairs can be calculated. Genes can then be clustered according to the similarity of the pattern of their interactions with all of the other genes to obtain a genetic interaction map, which can advantageously be used to predict functional associations between genes and identify drug targets for therapy such as combination cancer therapy.
Abstract translation: 本发明提供了使用核酸元件（例如干扰RNA）进行筛选以自信地鉴定命中遗传元件的方法。 本发明还包括构建含有两个或多个核酸元件的载体，以敲除从筛选鉴定的命中遗传元件的所有成对组合。 在单次和双重敲低表型的定量之后，可以计算所有基因对之间的遗传相互作用。 然后可以根据其与所有其他基因的相互作用模式的相似性将基因聚类，以获得遗传相互作用图，其可有利地用于预测基因之间的功能关联并鉴定用于治疗的药物靶标，例如组合癌症治疗 。
-
公开(公告)号：WO2021247570A8
公开(公告)日：2021-12-09
申请号：PCT/US2021/035244
申请日：2021-06-01
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： GILBERT, Luke , NUNEZ, James , WEISSMAN, Jonathan , CHEN, Jin
IPC: C12N9/22 , C07K19/00 , C12N9/10 , A61K47/65 , C12N9/1007
Abstract: Provided herein are, inter alia, fusion proteins, compositions and methods for manipulation of genomes of living organisms.
-
公开(公告)号：WO2021021677A1
公开(公告)日：2021-02-04
申请号：PCT/US2020/043608
申请日：2020-07-24
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： WEISSMAN, Jonathan , JOST, Marco , HORLBECK, Max , SANTOS, Daniel , SAUNDERS, Reuben
Abstract: The present disclosure provides methods and compositions for precisely controlling the expression levels of mammalian genes using CRISPRi or CRISPRa and one or more modified sgRNAs. The methods and compositions are useful for, inter alia, titrating the expression of a gene of interest, identifying drug targets and mechanisms of drug resistance, and enabling the analysis of and control over metabolic and signaling pathway fluxes.
-
9.发明申请ASSAYS FOR MASSIVELY COMBINATORIAL PERTURBATION PROFILING AND CELLULAR CIRCUIT RECONSTRUCTION 审中-公开用于大规模组合扰动分析和细胞电路重建的测定
公开(公告)号：WO2017075294A1
公开(公告)日：2017-05-04
申请号：PCT/US2016/059233
申请日：2016-10-27
Applicant: THE BOARD INSTITUTE INC. , MASSACHUSETTS INSTITUTE OF TECNOLOGY , THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , REGEV, Aviv , ADAMSON, Brittany , CLEARY, Brian , CONG, Le , DIXIT, Atray , GAUBLOMME, Jellert , LANDER, Eric, S. , NORMAN, Thomas , PAMAS, Oren , ROZENBLATT-ROSEN, Orit , SHALEK, Alexander, K. , WEISSMAN, Jonathan
Inventor： REGEV, Aviv , ADAMSON, Brittany , CLEARY, Brian , CONG, Le , DIXIT, Atray , GAUBLOMME, Jellert , LANDER, Eric, S. , NORMAN, Thomas , PAMAS, Oren , ROZENBLATT-ROSEN, Orit , SHALEK, Alexander, K. , WEISSMAN, Jonathan
IPC: G06F19/12
CPC classification number: G06F19/12
Abstract: The present invention provides tools and methods for the systematic analysis of genetic interactions, including higher order interactions. The present invention provides tools and methods for combinatorial probing of cellular circuits, for dissecting cellular circuitry, for delineating molecular pathways, and/or for identifying relevant targets for therapeutics development.
Abstract translation: 本发明提供了用于系统分析遗传相互作用（包括更高阶相互作用）的工具和方法。 本发明提供了用于组合探测细胞电路，用于解剖细胞电路，描绘分子途径和/或用于鉴定治疗学发展的相关靶标的工具和方法。
-
公开(公告)号：WO2014121104A1
公开(公告)日：2014-08-07
申请号：PCT/US2014/014213
申请日：2014-01-31
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： BRANDMAN, Onn , WEISSMAN, Jonathan
CPC classification number: C12N15/63
Abstract: Provided herein, inter alia, are methods for high throughput qualification and quantification of the effects of pooled genetic perturbations using perturbation elements.
Abstract translation: 本文提供了用于高通量鉴定和使用扰动元素的汇集遗传扰动的影响的量化方法。
-
-
-
-
-
-
-
-
-
Search Results
10
records
(took 0.032 seconds)
