公开(公告)号：WO2017062901A3

公开(公告)日：2017-05-26

申请号：PCT/US2016056172

申请日：2016-10-07

Applicant: TYMORA ANALYTICAL OPERATIONS LLC

Inventor： TAO ANDY ,  ILYUK ANTON ,  BAO NING

IPC: C12M1/42 ,  C12N13/00 ,  G01N27/00 ,  G01N30/00 ,  G01N33/50 ,  H03K3/64

CPC classification number: C12M1/34 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0816 ,  C12M23/16 ,  C12M33/00 ,  C12M47/16 ,  C12Q1/00

Abstract: EVs are connected to onset and progression of numerous diseases and can be used for their diagnosis. Methods and platform technology to isolate EVs and detect intravesicle phosphoproteins from biofluids are discussed. Secreted extracellular vesicles in biofluids are effectively isolated and phosphoproteins are extracted for detection and identification as biomarkers for disease diagnosis using antibodies, mass spectrometry, or other like methods.

Abstract translation: 电动车与许多疾病的发作和进展有关，可用于诊断。 讨论了分离电动汽车和检测生物体内液体磷脂蛋白的方法和平台技术。 有效分离生物流体中分泌的胞外囊泡，并提取磷蛋白用于检测和鉴定，作为使用抗体，质谱法或其他类似方法进行疾病诊断的生物标志物。