公开(公告)号：WO0244689A8

公开(公告)日：2002-11-14

申请号：PCT/US0145058

申请日：2001-11-28

Applicant: UNIV CALIFORNIA ,  WANG MARK ,  ATA ERHAN ,  ESENER SADIK

Inventor： WANG MARK ,  ATA ERHAN ,  ESENER SADIK

IPC: G21K1/00 ,  H05H3/04 ,  G01N

CPC classification number: H05H3/04

Abstract: Small particles 1, for example 5 mu m diameter microspheres or cells, within, and moving with, a fluid 2, normally water, that is flowing within microfluidic channels 3 within a radiation-transparent substrate 4, typically molded PDMS clear plastic, are selectively manipulated, normally by being pushed with optical pressures forces, with a laser light switching beam 5, preferably as arises from VCSELs operating in Laguerre-Gaussian mode, at branching junctions, such as an "X", in the microfluidic channels 3 so as to enter into selected downstream branches OUTPUT 1, OUPUT 2, thereby realizing switching and sorting of particles 1, including in parallel. Transport of the small particles 1 thus transpires by microfluidics while manipulation in the manner of optical tweezers arises either from pushing due to optical scattering force, or from pulling due to an attractive optical gradient force. Whether pushed or pulled, the particles within the flowing fluid may be optically sensed, and highly-parallel. Low-cost, cell- and particle-analysis devices efficiently realized, including as integrated on bio-chips.

Abstract translation: 在通常模制的PDMS透明塑料的辐射透明基底4内流动的微流体通道3内流动的流体2（通常为水）内的微粒1，例如5μm直径的微球或细胞，是选择性的 通常通过用光学压力推动的激光切换光束5，优选地如在来自微型流体通道3中的分支结点（例如“X”）由在Laguerre-Gaussian模式下操作的VCSEL产生的，以便 进入选择的下游分支OUTPUT 1，OUPUT 2，从而实现并行包括颗粒1的切换和分类。 因此，通过微流体传输小颗粒1，同时以光学镊子的方式进行操作会由于光学散射力的推动或由于有吸引力的光学梯度力的拉动而产生。 无论是推动还是被拉动，流动流体中的颗粒可以被光学感测并且高度平行。 低成本，细胞和粒子分析装置有效实现，包括集成在生物芯片上。

公开(公告)号：WO0244689A3

公开(公告)日：2003-04-24

申请号：PCT/US0145058

申请日：2001-11-28

Applicant: UNIV CALIFORNIA ,  WANG MARK ,  ATA ERHAN ,  ESENER SADIK

Inventor： WANG MARK ,  ATA ERHAN ,  ESENER SADIK

IPC: G21K1/00 ,  H05H3/04 ,  C12Q1/68 ,  B21D33/00 ,  C12M1/34 ,  G01N33/543 ,  G02B6/42

CPC classification number: H05H3/04

Abstract: The invention contemplates the use of optical beams, as generate photonic pressure, or radiation pressure, to perform switching of small particles that are flowing in microfluidic channels. Small particles (1), for example 5 um diameter water microspheres or cells, within and moving with, a fluid (2), normally water, that is flowing within microfluidic channels (3) within a radiation transparent substrate (4) being pushed with optical pressure forces with a laser light switching beam (5), preferably as arises from VCSELs operating in Laguerre-Gaussian mode, at branching junctions such as an "X" in the microfluidic channels (3) so as to enter into selected downstream branches OUTPUT 1 & 2, thereby realizing switching and storing of particles (1) including parallel.

Abstract translation: 本发明考虑使用光束作为产生光子压力或辐射压力来执行在微流体通道中流动的小颗粒的切换。 在辐射透明基板（4）内的微流体通道（3）内流动的流体（2）（通常为水）内并与其一起移动的小颗粒（1），例如5微米直径的水微球或细胞， 具有激光切换光束（5）的光学压力，优选地源于在拉格鲁斯 - 高斯模式下操作的VCSEL，在微流体通道（3）中的诸如“X”的分支连接处进入选择的下游分支输出 1和2，从而实现包括并行的颗粒（1）的切换和存储。

公开(公告)号：WO2004033059A3

公开(公告)日：2006-01-26

申请号：PCT/US0330975

申请日：2003-09-30

Applicant: GENOPTIX INC ,  ZHANG HAICHUAN ,  CHUNG THOMAS D Y ,  HALL JEFF ,  SOOHOO WILLIAM ,  KOHRUMEL JOSH ,  TU EUGENE ,  WANG MARK ,  RAYMOND DANIEL EDWARD ,  MARCHAND PHILIPPE ,  DIVER JONATHAN ,  BUTLER WILLIAM F ,  NGUYEN PHAN ,  CHACHISVILIS MIRIANAS ,  KATZ ANDREW ,  HAGEN NORBERT ,  LYKSTAD KRIS ,  PESTANA LUIS

Inventor： ZHANG HAICHUAN ,  CHUNG THOMAS D Y ,  HALL JEFF ,  SOOHOO WILLIAM ,  KOHRUMEL JOSH ,  TU EUGENE ,  WANG MARK ,  RAYMOND DANIEL EDWARD ,  MARCHAND PHILIPPE ,  DIVER JONATHAN ,  BUTLER WILLIAM F ,  NGUYEN PHAN ,  CHACHISVILIS MIRIANAS ,  KATZ ANDREW ,  HAGEN NORBERT ,  LYKSTAD KRIS ,  PESTANA LUIS

IPC: G01N33/48 ,  G01N33/569 ,  G21K1/00 ,  C12Q1/00 ,  C12M1/00 ,  G01F23/00 ,  G01N33/558

CPC classification number: G01N33/56966 ,  G01N15/147 ,  G01N15/1484 ,  G01N2015/1006 ,  G01N2015/1075 ,  G01N2015/145

Abstract: A device for characterizing a cell or particle includes a channel having an inlet and an outlet, the channel containing a moving fluid therein for carrying the cell or particle from the inlet to the outlet. The device includes detectors for monitorng the position of the cell or particle along the channel. The device further comprises a light source to provide an optical gradient and a control system to receive and process signals from the detector.The device can be used to characterize and sort cells based on a biological property.

Abstract translation: 用于表征细胞或颗粒的装置包括具有入口和出口的通道，所述通道在其中包含运动流体，用于将细胞或颗粒从入口运送到出口。 该装置包括用于监测细胞或颗粒沿着通道的位置的检测器。 该装置还包括提供光学梯度的光源和用于接收和处理来自检测器的信号的控制系统。该装置可用于基于生物特性来表征和分类细胞。

公开(公告)号：WO2009099512A3

公开(公告)日：2009-10-08

申请号：PCT/US2009000272

申请日：2009-01-16

Applicant: MICROPOINT BIOSCIENCES INC ,  ZHANG NAN ,  WANG MARK

Inventor： ZHANG NAN ,  WANG MARK

IPC: G01N35/02 ,  G01N33/48 ,  G01N35/10

CPC classification number: G01N35/00069 ,  G01N2035/00257 ,  G01N2035/00495 ,  Y10T436/2575

Abstract: This invention provides rotors and methods of precisely metering a sample fluid and mixing the sample with a reagent. The rotors have a metering tube of defined volume that fills until sample flow is stopped by surface tension of a meniscus at a capillarity port, while excess sample is stripped from the metering tube inlet by centripetal force of the spinning rotor. By spinning the rotor at a higher speed, a reagent can be forced from a reagent chamber to contact the meniscus, breaking the surface tension and allowing the metered sample to mix with the reagent.

Abstract translation: 本发明提供了精确计量样品流体并将样品与试剂混合的转子和方法。 转子具有限定容积的计量管，该计量管填充直到样品流由毛细管口处的弯月面的表面张力停止，而过量样品通过旋转转子的向心力从计量管入口剥离。 通过以更高的速度旋转转子，可以从试剂室强制试剂接触弯月面，破坏表面张力并使计量的样品与试剂混合。

公开(公告)号：WO2007062039A2

公开(公告)日：2007-05-31

申请号：PCT/US2006045058

申请日：2006-11-21

Applicant: ILLUMINA INC ,  FENG WENYI ,  KOTSEROGLOU THEOFILOS ,  WANG MARK ,  TRIENER ALEXANDER ,  CHE DIPING ,  KAIN ROBERT

Inventor： FENG WENYI ,  KOTSEROGLOU THEOFILOS ,  WANG MARK ,  TRIENER ALEXANDER ,  CHE DIPING ,  KAIN ROBERT

IPC: G02B21/00 ,  G01N21/64

CPC classification number: G01N21/6456 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6421 ,  G02B21/0032 ,  G02B21/004

Abstract: The invention provides imaging apparatus and methods useful for obtaining a high resolution image of a sample at rapid scan rates. A rectangular detector array having a horizontal dimension that is longer than the vertical dimension can be used along with imaging optics positioned to direct a rectangular image of a portion of a sample to the rectangular detector array. A scanning device can be configured to scan the sample in a scan-axis dimension, wherein the vertical dimension for the rectangular detector array and the shorter of the two rectangular dimensions for the image are in the scan-axis dimension, and wherein the vertical dimension for the rectangular detector array is short enough to achieve confocality in a single axis.

Abstract translation: 本发明提供了用于以快速扫描速率获得样品的高分辨率图像的成像装置和方法。 具有比垂直尺寸长的水平尺寸的矩形检测器阵列可以与定位成将样本的一部分的矩形图像引导到矩形检测器阵列的成像光学元件一起使用。 扫描装置可以被配置成以扫描轴尺寸扫描样本，其中矩形检测器阵列的垂直尺寸和图像的两个矩形尺寸中的较短的尺寸在扫描轴尺寸上，并且其中垂直尺寸 因为矩形检测器阵列足够短以在单个轴上实现共焦。