公开(公告)号：WO1994025607A1

公开(公告)日：1994-11-10

申请号：PCT/US1994004379

申请日：1994-04-20

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： UNIVERSITY OF IOWA RESEARCH FOUNDATION ,  TEEM, John, L.

IPC: C12N15/62

CPC classification number: G01N33/6893 ,  C07K14/395 ,  C07K14/4712 ,  C07K2319/80 ,  G01N2500/00 ,  G01N2800/382

Abstract: A method of identifying potentially useful treatments which restore the function of CFTR or other related protein mutations is disclosed. The method involves creation of yeast STE6-CFTR chimeric sequences which upon introduction of a desired mutation inhibit expression of the yeast STE6 gene. A yeast assay (STE6 DELTA ) transformed to include the hybrid chimeras is then exposed to a potential treatment for the mutation. A treatment which restores the function of the STE6 gene and thereby allows mating of the yeast strain is indicative of a potential remedial treatment for the mutation in this selected protein.

Abstract translation: 公开了一种鉴定恢复CFTR或其他相关蛋白突变功能的潜在有用治疗方法。 该方法包括产生酵母STE6-CFTR嵌合序列，其在引入所需突变时抑制酵母STE6基因的表达。 转化成包含杂交嵌合体的酵母测定（STE6 DELTA）然后暴露于潜在的突变处理。 恢复STE6基因功能从而允许酵母菌株交配的治疗指示了对该选择的蛋白质中突变的潜在补救处理。

公开(公告)号：WO1992002816A1

公开(公告)日：1992-02-20

申请号：PCT/US1991005215

申请日：1991-07-23

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： UNIVERSITY OF IOWA RESEARCH FOUNDATION ,  LINHARDT, Robert, J. ,  LEE, Kyung-Bok ,  AL-HAKIM, Ali ,  LOGANATHAN, Duraikkannu

IPC: G01N33/52

CPC classification number: G01N33/52 ,  C12Q1/34 ,  G01N2333/924 ,  Y10T436/143333

Abstract: The electrophoretic isolation and monosaccharide sequence determination of a neutral or weakly acidic oligosaccharide species of interest are disclosed. A labeling compound and a charged group are coupled to the reducing end of the species of interest, thereby facilitating electrophoretic separation and detection of the separated species. The resolved species of interest can then be recovered from the electrophoretic medium, for example, by electrophoretic transfer to a charged solid support. Following isolation, monosaccharide units can be cleaved successively from the non-reducing end of the species of interest to reveal the monosaccharide sequence. The identity of each monosaccharide unit is determined by correlating cleavage data with known exoglycosidase specificites.

公开(公告)号：WO1992000287A2

公开(公告)日：1992-01-09

申请号：PCT/US1991003928

申请日：1991-06-03

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： UNIVERSITY OF IOWA RESEARCH FOUNDATION ,  SCHOENWALD, Ronald, D. ,  BARFKNECHT, Charles, F.

IPC: C07D285/135

CPC classification number: C07D285/135 ,  C07D277/80

Abstract: Analogs are prepared of the carbonic anhydrase inhibitors methazolamide, and dichlorphenamide. The analogs link a cleavable functional group to the pharmacologically active carbonic anhydrase inhibitor resulting in compounds of improved corneal permeability, which are non-irritable to the eye and which are effective topically to inhibit intraocular pressure.

Abstract translation: 类似物由碳酸酐酶抑制剂甲唑胺和二氯苯酰胺制备。 类似物将可切割的官能团与药理活性的碳酸酐酶抑制剂连接，导致改善的角膜通透性的化合物，其对眼睛是不易刺激的，并且在局部有效地抑制眼内压。

公开(公告)号：WO1991018107A1

公开(公告)日：1991-11-28

申请号：PCT/US1991003632

申请日：1991-05-23

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION ,  CAMPBELL, Kevin, P. ,  ERVASTI, James, M. ,  OHLENDIECK, Kay ,  GAVER, Mitchell, G. ,  KAHL, Steven, D.

Inventor： UNIVERSITY OF IOWA RESEARCH FOUNDATION

IPC: C12P21/08

CPC classification number: C07K16/28 ,  A61K38/00 ,  A61K39/00 ,  C07K14/4707 ,  C07K14/705 ,  C07K16/18 ,  G01N33/6887 ,  G01N2800/2878 ,  G01N2800/2885 ,  Y10S436/811

Abstract: The invention pertains to the dystrophin-glycoprotein complex of mammalian skeletal muscle and a method of isolating said complex. The components of the complex and methods of separating and isolating said components also pertain to the invention. In addition, the invention further relates to a method of detecting and quantifying the loss of a non-dystrophin component of the dystrophin-glycoprotein complex with said loss being indicative of muscular dystrophy.

公开(公告)号：WO1991017255A1

公开(公告)日：1991-11-14

申请号：PCT/US1991003094

申请日：1991-05-06

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： UNIVERSITY OF IOWA RESEARCH FOUNDATION ,  DORDICK, Jonathan, S. ,  RETHWISCH, David, G. ,  PATIL, Damodar, R.

IPC: C12P19/14

CPC classification number: C08B37/00 ,  C08G63/12 ,  C12P7/62 ,  C12P19/00 ,  C12P19/02 ,  C12P19/04 ,  C12P19/44

Abstract: A method of making a sugar-based polymer is disclosed. A sugar and an organic acid derivative having at least two carboxylate functionalities are mixed in a substantially non-aqueous organic solvent with a hydrolytic enzyme and, thereafter, the resulting mixture is agitated for a time sufficient to allow for the polymerization of the sugar. The resulting sugar-based polymer has sugar incorporated in the polymer backbone.

Abstract translation: 公开了制备糖基聚合物的方法。 将具有至少两个羧酸酯官能团的糖和有机酸衍生物在基本上不含水的有机溶剂中与水解酶混合，然后将所得混合物搅拌足以允许糖的聚合的时间。 所得的糖基聚合物在聚合物骨架中掺入糖。

公开(公告)号：WO1991013077A1

公开(公告)日：1991-09-05

申请号：PCT/US1991001124

申请日：1991-02-20

Applicant: THE SALK INSTITUTE BIOTECHNOLOGY/INDUSTRIAL ... ,  UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： THE SALK INSTITUTE BIOTECHNOLOGY/INDUSTRIAL ... ,  UNIVERSITY OF IOWA RESEARCH FOUNDATION ,  JAY, Scott, David ,  ELLIS, Steven, Bradley ,  HARPOLD, Michael, Miller ,  CAMPBELL, Kevin, Peter

IPC: C07H15/12

CPC classification number: C07K14/705 ,  C12Q1/6876

Abstract: Calcium channel gamma -subunit-encoding cDNAs, and related compositions and methods, are provided.

Abstract translation: 提供钙通道γ-亚基编码cDNA，以及相关组合物和方法。

公开(公告)号：WO1989010966A1

公开(公告)日：1989-11-16

申请号：PCT/US1989001850

申请日：1989-05-01

Applicant: THE CHILDREN'S HOSPITAL, INCORPORATED ,  UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： THE CHILDREN'S HOSPITAL, INCORPORATED ,  UNIVERSITY OF IOWA RESEARCH FOUNDATION ,  STINSKI, Mark, F. ,  GEHRZ, Richard, C.

IPC: C12N15/00

CPC classification number: C07K14/005 ,  A61K39/00 ,  C12N2710/16122

Abstract: A recombinant expression vector is provided which comprises DNA derived from the genome of human cytomegalovirus (HCMV), such as HXLF 1-5, wherein said DNA encodes a polypeptide which represents at least a portion of the gcII HCMV glycoprotein complex.

Abstract translation: 提供了重组表达载体，其包含衍生自人巨细胞病毒（HCMV）的基因组的DNA，例如HXLF 1-5，其中所述DNA编码代表gcII HCMV糖蛋白复合物的至少一部分的多肽。

公开(公告)号：WO1985001514A1

公开(公告)日：1985-04-11

申请号：PCT/US1984001263

申请日：1984-08-13

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： UNIVERSITY OF IOWA RESEARCH FOUNDATION ,  RHEAD, William, J. ,  FRITCHMAN, Kathie, S. ,  STOUT, Ferris, M. ,  MELTON, Hewlett

IPC: C12N13/00

CPC classification number: C12M33/12 ,  C12M23/08 ,  C12M27/12 ,  C12N1/02 ,  C12N13/00 ,  H01L31/1844 ,  Y02E10/544

Abstract: A technique for harvesting mammalian cells from an artificial substrate using ultrasonic energy in the range of 5 to 20 joules/sq. cm., at an intensity in the range of 30 to 150 mW/sq. cm, and at a frequency in the range of 20 to 60 kHz.

Abstract translation: 使用5至20焦耳/平方厘米范围内的超声波能量从人造底物收集哺乳动物细胞的技术。 cm 3，强度为30〜150mW / sq。 cm，频率范围为20〜60kHz。

公开(公告)号：WO1998031652A1

公开(公告)日：1998-07-23

申请号：PCT/US1997024054

申请日：1997-12-30

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： UNIVERSITY OF IOWA RESEARCH FOUNDATION ,  BAKER, Max., T. ,  TINKER, John, H. ,  RUZICKA, Jan, A.

IPC: C07C41/00

CPC classification number: C07C41/22 ,  C07C43/12 ,  C07C43/123

Abstract: A method of synthesizing 1,1,1,3,3,3-hexafluoroisopropyl ether compounds, including, or such as sevoflurane, from methoxymalononitrile, a compound also known as alpha , alpha -dicyanomethyl methyl ether, and having the formula (CN)2CHOCH3, and having the chemical structure (I).

Abstract translation: 一种合成1,1,1,3,3,3-六氟异丙基醚化合物的方法，其中包括七氟烷，或由七氟醚，甲氧基丙二腈，也称为α，α-二氰基甲基甲基醚，并具有式（CN） 2CHOCH 3，并且具有化学结构（I）。

公开(公告)号：WO1998020131A1

公开(公告)日：1998-05-14

申请号：PCT/US1997020702

申请日：1997-11-07

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： UNIVERSITY OF IOWA RESEARCH FOUNDATION ,  STONE, Edwin, M. ,  SHEFFIELD, Val ,  ALWARD, Wallace, L., M.

IPC: C12N15/12

CPC classification number: C07K14/47 ,  A01K2217/05 ,  A61K38/00

Abstract: Diagnostics for glaucoma, which are based on detection of non-wildtype GLC1A genes or proteins in a subject, glaucoma therapeutics and assays for identifying agents that modulate the activity of GLC1A and that are therefore useful in treating or preventing glaucoma are described.

Abstract translation: 描述了基于检测受试者中非野生型GLC1A基因或蛋白质的青光眼的诊断，青光眼治疗剂和用于鉴定调节GLC1A活性并因此可用于治疗或预防青光眼的药剂的测定。